Multi-enzyme active metal-based cuttlefish ink melanin composite material and preparation method thereof

A technology of composite material and melanin, which is applied in the field of metal-based squid ink melanin composite material and its preparation, can solve problems such as limitations, and achieve the effects of low price, convenient operation, and simple preparation process

Pending Publication Date: 2022-05-06
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the squid ink melanin used in the above composites is still limited to the bold form after washing and centrifugation

Method used

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  • Multi-enzyme active metal-based cuttlefish ink melanin composite material and preparation method thereof
  • Multi-enzyme active metal-based cuttlefish ink melanin composite material and preparation method thereof
  • Multi-enzyme active metal-based cuttlefish ink melanin composite material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Cu-doped composites with multi-enzyme activity using cuttlefish ink as raw material.

[0033] (1) Cut the cuttlefish ink sac and soak the ink sac juice in a solution of ethanol (95%) and water at a volume of 1:1 overnight to remove surface impurities. The precipitate was washed several times with deionized water, and then dried in an oven at 60° C. for 12 h. Take the dried cuttlefish juice melanin and crush it into powder with a wall breaking machine, and pass it through a 200-mesh sieve for use;

[0034] (2) Dissolve 5 g of pre-broken squid ink melanin after sieving in 50 mL of 1 mmol / L sodium hydroxide solution, heat to 80 ° C for 2 h; dialyze the cooled mixed solution, and then dialyze The mixture in the bag was centrifuged at 150r / min for 8mi, and the supernatant was dried in an oven at 60°C to obtain purified cuttlefish ink melanin.

[0035] (3) Doping: Disperse 0.5g of purified cuttlefish ink melanin in 10mmol / L Cu 2+ 100mmol / L sodium chloride sa...

Embodiment 2

[0037] Preparation of Fe-doped composites with multi-enzyme activity using cuttlefish ink as raw material.

[0038] (1) Cut the cuttlefish ink sac and soak the ink sac juice in a solution of ethanol (95%) and water at a volume of 1:1 overnight to remove surface impurities. The precipitate was washed several times with deionized water, and then dried in an oven at 60° C. for 12 h. Take the dried cuttlefish juice melanin and crush it into powder with a wall breaking machine, and pass it through a 200-mesh sieve for use;

[0039] (2) Dissolve 5 g of pre-broken squid ink melanin after sieving in 50 mL of 1 mmol / L sodium hydroxide solution, heat to 80 ° C for 2 h; dialyze the cooled mixed solution, and then dialyze The mixture in the bag was centrifuged at 150r / min for 8mi, and the supernatant was dried in an oven at 60°C to obtain purified cuttlefish ink melanin.

[0040] (3) Doping: disperse 0.5g of purified squid ink melanin in a solution containing 10mmol / LFe 3+100mmol / L sod...

Embodiment 3

[0042] Preparation of Mn-doped composites with multi-enzyme activity using cuttlefish ink as raw material.

[0043] (1) Cut the cuttlefish ink sac and soak the ink sac juice in a solution of ethanol (95%) and water at a volume of 1:1 overnight to remove surface impurities. The precipitate was washed several times with deionized water, and then dried in an oven at 60° C. for 12 h. Take the dried cuttlefish juice melanin and crush it into powder with a wall breaking machine, and pass it through a 200-mesh sieve for use;

[0044] (2) Dissolve 5 g of pre-broken squid ink melanin after sieving in 50 mL of 1 mmol / L sodium hydroxide solution, heat to 80 ° C for 2 h; dialyze the cooled mixed solution, and then dialyze The mixture in the bag was centrifuged at 150r / min for 8mi, and the supernatant was dried in an oven at 60°C to obtain purified cuttlefish ink melanin.

[0045] (3) Doping: disperse 0.5g of purified cuttlefish ink melanin in 10mmol / LMn 2+ 100mmol / L sodium chloride sal...

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Abstract

The invention discloses a metal-based cuttlefish ink melanin composite material with multi-enzyme activity and a preparation method thereof. According to the technical scheme, the composition comprises purified cuttlefish ink melanin and metal loaded on the surface of the melanin; the purified cuttlefish ink melanin is obtained through the steps of cleaning, crushing, sieving and alkali decomposition. The composite material has one or more activities of peroxidase, oxidases, catalase and superoxide dismutase, and can be used as mimic enzymes of peroxidase, oxidases, catalase and superoxide dismutase. Compared with a traditional preparation method, the biomass cuttlefish ink melanin which is large in stock, low in price and environmentally friendly is adopted as the raw material, the preparation process is simple, no toxic reagent is involved, and large-scale production can be achieved.

Description

technical field [0001] The invention belongs to the technical field of catalysts, and in particular relates to a metal-based cuttlefish ink melanin composite material with a function of simulating enzyme catalysis and a preparation method thereof. Background technique [0002] Natural enzymes play an important role in all life activities and have been widely used in various fields due to their excellent performance. However, the inherent disadvantages of natural enzymes, such as high cost and low stability, severely limit their practical applications. In order to overcome these shortcomings, people vigorously develop high-performance natural enzyme substitutes. In the past few decades, a variety of biomimetic nanomaterials have been proposed with enzyme-like activities, which can serve as high-performance substitutes for natural enzymes. In general, natural enzymes are mainly composed of a protein part and a metal cofactor part. The protein part, that is, the peptide chai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/28B01J31/32C09B61/00C09B67/54
CPCB01J31/28B01J31/32C09B61/00C09B67/0096
Inventor 周丽华黄晓桐陈颖欣袁勇
Owner GUANGDONG UNIV OF TECH
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