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Rapid enrichment culture method of denitrifying phosphorus removal bacteria

A technology for denitrifying phosphorus removal bacteria and enrichment culture, which is applied in the field of rapid enrichment culture of denitrifying phosphorus removal bacteria, can solve the problems of long culture, slow establishment of denitrification phosphorus removal process, etc., achieves precise control process and realizes selection The effect of rapid proliferation and elimination of culture time

Active Publication Date: 2022-05-03
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, step (1) of this method needs to run for 90 days, and the cultivation is relatively long, which leads to the slow establishment of the denitrification and phosphorus removal process

Method used

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  • Rapid enrichment culture method of denitrifying phosphorus removal bacteria
  • Rapid enrichment culture method of denitrifying phosphorus removal bacteria
  • Rapid enrichment culture method of denitrifying phosphorus removal bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Enhancement of sludge phosphorus removal performance and determination of ORP inflection point in culture system

[0043] Preparation of culture medium S1: COD and ammonia nitrogen concentrations were 200mg / L and 5.0mg / L respectively, prepared by NaAc and NH 4 Cl provided. At the same time, add CaCl 2 0.03g / L, MgSO 4 0.06g / L, FeSO 4 0.0012g / L, trace element nutrient solution 1.25g / L. Each liter of trace element nutrient solution contains EDTA (10g), FeCl 3 •6H 2 O (1.5g), ZnSO 4 •7H 2 O (0.12g), MnCl 2 • 4H 2 O (0.12g), H 3 BO 3 (0.15g), KI (0.18g), Na 2 MoO 4 • 2H 2 O (0.06g), CuSO 4 • 5H 2 O (0.03g), CoCl 2 •6H 2 O (0.15g). The pH is about 7.2.

[0044] Preparation of culture medium S2: NO 3 - -N concentration is 50mg / L, by NaNO 3 supply. Phosphorus concentration is added according to the same concentration of phosphorus in the culture solution at the end of the phosphorus release period, and the KH 2 PO 4 supply. Among them ammonia nitr...

Embodiment 2

[0055] Same as Example 1, the difference is that the concentrations of COD and ammonia nitrogen in culture solution S1 are 300 mg / L and 10 mg / L respectively, the concentration of nitrate nitrogen in culture solution S2 is 70 mg / L, and the concentration of P is 40 mg / L.

[0056] (1) Enhancement of sludge phosphorus removal performance and determination of ORP inflection point in culture system

[0057] The inflection point ORP1=-164mV is determined in the anaerobic stage, and the inflection point ORP2=-223mV is determined in the anoxic stage. When denitrification phosphorus removal bacteria accounted for 23% of all phosphorus removal bacteria, the sludge phosphorus removal performance enhancement process was completed.

[0058] (2) Cultivate denitrification and phosphorus removal bacteria

[0059] According to the ORP1 and ORP2 determined during the strengthening process, the PLC is set to start enriching denitrifying and phosphorus-removing bacteria. The culture medium used ...

Embodiment 3

[0062] Same as Example 1, except that the COD and ammonia nitrogen concentrations in the culture solution S1 are 100 mg / L and 3 mg / L respectively, the nitrate nitrogen concentration in the culture solution S2 is 50 mg / L, and the P concentration is 10 mg / L.

[0063] (1) Enhancement of sludge phosphorus removal performance and determination of ORP inflection point in culture system

[0064] The inflection point ORP1=-191mV is determined in the anaerobic stage, and the inflection point ORP2=-229mV is determined in the anoxic stage. When denitrification phosphorus removal bacteria accounted for 22% of all phosphorus removal bacteria, the sludge phosphorus removal performance enhancement process was completed.

[0065] (2) Cultivate denitrification and phosphorus removal bacteria

[0066] According to the ORP1 and ORP2 determined during the strengthening process, the PLC is set to start enriching denitrifying and phosphorus-removing bacteria. The culture medium used in the enrich...

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Abstract

The invention relates to a rapid enrichment culture method of denitrifying phosphorus removal bacteria, which comprises the following steps: (1) strengthening the phosphorus removal performance of sludge: firstly, carrying out anaerobic culture and determining an inflection point ORP1, and draining water until the culture is finished; then carrying out anoxic culture, determining an inflection point ORP2, draining water until the culture is finished, and alternately carrying out the steps, and ending the strengthening process when the denitrifying phosphorus removal bacteria account for more than 20% of all phosphorus removal bacteria; and (2) enrichment culture of denitrifying phosphorus removal bacteria: taking ORP1 and ORP2 as switching points of anaerobic culture and anoxic culture, carrying out alternate culture, and finishing enrichment culture when the denitrifying phosphorus removal bacteria account for more than 70% of all phosphorus removal bacteria. According to the method, a two-step combined culture mode of sludge phosphorus removal performance enhancement and denitrifying phosphorus removal bacterium enrichment culture is adopted, and accurate switching of different stages is realized by combining ORP as a control parameter, so that rapid enrichment culture of thalli is realized.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a rapid enrichment culture method for denitrification and phosphorus removal bacteria. Background technique [0002] At present, the problem of "eutrophication" in the water body environment is becoming more and more serious, and the national sewage discharge standard is continuously improved. In order to control the eutrophication of the water body, the sewage treatment technology of nitrogen and phosphorus removal has become one of the research hotspots in the field of sewage treatment. Compared with physical and chemical methods, biological treatment has the advantages of low cost and less secondary pollution, so biological nitrogen and phosphorus removal technology has always been the main research object in the field of sewage treatment. The traditional biological nitrogen and phosphorus removal process has achieved certain results in the biological ni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
CPCC02F3/34C02F2203/004C02F2209/04C02F2209/06C02F2209/42C02F2209/02C02F2209/22C02F2305/06Y02W10/10
Inventor 王刚高会杰孙丹凤陈明翔郭志华
Owner CHINA PETROLEUM & CHEM CORP
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