Composition of melanoidins for protecting crops from non-fungal pests
A composition and technology of melanoidin, applied in plant cultivation, animal repellent, plant phenotype improvement, etc., can solve the problems of toxin use concentration limitation, difficult plant protection, complex plant protection, etc.
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Embodiment 1
[0073] Tracking melanoid formation
[0074] Maillard reactions are a class of non-enzymatic browning reactions that involve the interaction of a reducing sugar with a free amino acid or the free amino group of an amino acid that is part of a protein chain. It is well known that the reaction is influenced by the temperature and composition of the reaction mixture, whereas UV-absorbing and colorless intermediates are formed in the initial stage and oligomeric or polymeric brown melanoidins are formed in the final stage. Melanoidins can be quantified by measuring absorbance at 420 nm as a measure of "browning" or by measuring absorbance at other wavelengths as a response track. Figure 8A The UV-Vis absorption spectra of twelve samples are shown, which were obtained by: i) heating a solid mixture of glucose and glutamic acid in a molar ratio of 1:1 (called M11), ground and heated at 170 °C Heating in oven for different periods of time: 0, 2, 4, 6, 8, 10, 10.5, 11, 12, 14 and 1...
Embodiment 2
[0082] Plant and Disease Evaluation
[0083] Typically, tomato plants are grown from seed in a nursery and transplanted into 1-liter pots 40 to 50 days after sowing. Plants were fertilized with NPK fertilizer (irrigation water aimed at total N, P and K concentrations of 120, 30 and 150 mg / L, respectively). Plants are usually kept in a pest-free and disease-free greenhouse during growth at 20-30°C, under natural light and a relative humidity of 50-90%, and then transferred to pathogen infection on intact or detached leaves as described below later diseased areas. Disease severity was evaluated for each plant based on % coverage, where 0% was defined as all leaves were free of disease symptoms and 100% was defined as all leaves were completely covered by disease symptoms. Always use the same coverage.
Embodiment 3
[0085] virus disease
[0086] Tomato Brown Ruffle Virus (TBRFV) was maintained on tomato plants. For inoculation, the infected plant parts were blended into small pieces in tap water using a blender, and the crushed plant mass was sprayed with emery powder on the treated tomato plants. Plants were kept in a greenhouse at 18-28°C. Disease severity was evaluated for each plant based on % coverage, where 0% was defined as all leaves were free of disease symptoms and 100% was defined as all leaves were completely covered by disease symptoms.
[0087] Symptoms caused by TBRFV include a mosaic pattern on the leaves, occasionally with narrowing of the leaves and yellow speckled wrinkled fruit. TBRFV is a virus belonging to the genus Tobamovirus. It was found that spraying the melanoidin composition of the present invention onto tomato plants or saturating the root zone of tomato plants with the composition significantly suppressed the severity of viral infection as manifested on...
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