Multi-target simultaneous detection method based on latex microsphere ultraviolet absorption spectrum peak area integration
A technology of latex microspheres and area integration, which is applied in the field of biochemical analysis, can solve problems such as failure to meet amplification requirements, inactivation of biorecognition molecules, and weakening signal gaps, etc., to avoid baseline background interference, high accuracy, and small range Effect
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Embodiment 1
[0083] Multiple Quantitative Detection of Example 1 Deoxynivalenol (DON), Aflatoxin (AFB) and Ochratoxin (OTA)
[0084] Simultaneous detection of vomitoxin, aflatoxin and ochratoxin by competitive immunoassay, the schematic diagram is as follows Figure 9 As shown, magnetic particles with a particle size of 1000nm were used as magnetic separation carriers, and antibodies corresponding to vomitoxin, aflatoxin, and ochratoxin were coupled to the surface; 99nm, 500nm, and 1050nm PS microspheres were used as signal probes, respectively. The complete antigen coupled with three targets, when the target exists, the complete antigen will compete with the target for the antibody on the surface of the carrier to form nano-magnetic particles-antibody-target, nano-magnetic particles-antibody-complete antigen-PS microparticles Sphere complexes and unreacted signaling probe solutions. Therefore, the content of the target directly affects the number of signal probe-carrier complexes formed....
Embodiment 2
[0096] Example 2 Multivariate signal amplification method for deoxynivalenol (DON), aflatoxin (AFB) and ochratoxin (OTA)
[0097] Simultaneously improve the sensitivity of analyzing vomitoxin, aflatoxin and ochratoxin, the schematic diagram is as follows Figure 11 As shown, magnetic particles with a particle size of 1000nm are used as magnetic separation carriers, and antibodies corresponding to vomitoxin, aflatoxin, and ochratoxin are coupled on the surface; ;Using 99nm, 500nm, 1050nm PS microspheres as signal probes, respectively coupled with click reagent II corresponding to click reagent I, when the target exists, the complete antigen will compete with the target for the antibody on the surface of the carrier to form nano Magnetic particle-antibody-target object, magnetic nanoparticle-antibody-complete antigen-click reagent I-click reagent II-PS microsphere complex and signal probe solution not involved in the reaction. Therefore, the content of the target directly affec...
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