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Method for promoting bisphenol A conversion by laccase-induced copolymerization

A technology of copolymerization and laccase, which is applied in the field of cross fusion, can solve the problems of decreased catalytic activity of laccase, hindering the enzymatic reaction rate and removal effect of BPA monomer, and inactivation, so as to reduce the mineralization of microorganisms and eliminate biological Toxic effects, effects of rapid oxidation and removal

Pending Publication Date: 2022-04-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

It should be emphasized that with the increase of the time and degree of BPA polymerization induced by Yunzhi laccase, the formation of long-chain BPA self-polymers will gradually increase, which will lead to a significant decrease or even inactivation of the catalytic activity of laccase, thereby seriously hindering the synthesis of BPA monopolymerization. Subsequent enzymatic reaction rate and removal effect

Method used

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  • Method for promoting bisphenol A conversion by laccase-induced copolymerization
  • Method for promoting bisphenol A conversion by laccase-induced copolymerization
  • Method for promoting bisphenol A conversion by laccase-induced copolymerization

Examples

Experimental program
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Effect test

Embodiment 1H

[0021] Example 1 Effect of HA on the oxidation and removal of BPA induced by versicolor laccase

[0022] To investigate the effect of HA concentration on the conversion of BPA induced by T.versicolor laccase. The key experimental steps are briefly described as follows: (1) A 50mL heat-resistant brown glass bottle was selected as a container for laccase to initiate free radical polymerization, and the container contained 20mL of 10mM citric acid-phosphate buffer, 100μM BPA and 0-50 (ie 0, 10, 20, 30, 40 and 50) mg·L -1 HA; (2) Accurately add 2U·mL to the buffer -1 Yunzhi laccase (light brown, ≥0.5U·mg -1 ), start the enzymatic polymerization reaction; (3) the condition of free radical polymerization initiated by Yunzhi laccase is pH 5.0, 25°C, and dark conditions, and stand for oxidative polymerization for 3 hours; (4) In addition, after 3 hours of enzymatic reaction, Add 30mg·L to the system -1 HA, to further study the effect of HA addition sequence on laccase-induced BPA ...

Embodiment 2

[0024] The influence of embodiment 2HA on BPA autopolymer output and distribution

[0025] Using LTQ-Orbitrap high-resolution mass spectrometry (HRMS, Thermo Fisher Scientific, Bremen, Germany; molecular mass accuracy -1) on the production and distribution of BPA autopolymer induced by Yunzhi laccase. A total of three macromolecular BPA self-polymers were screened out, and their m / z were 453.2071, 679.3054 and 905.4067, respectively. Based on the precise molecular masses and elemental compositions of the three compounds, these products were preliminarily identified as BPA dimers (C 30 h 30 o 4 ), trimer (C 45 h 44 o 6 ) and tetramers (C 60 h 58 o 8 ) (molecular mass accuracy <2.0ppm). It should be pointed out that the generation of small molecular BPA degradation products and supramolecular BPA-HA copolymers were not detected. Mainly because of the complexity and uncertainty of the structure of HA, it is difficult to reveal the molecular mass and chemical structure o...

Embodiment 3

[0027] The physical and chemical properties of embodiment 3 supramolecular BPA-HA copolymer

[0028] A large number of supramolecular BPA-HA copolymer particle products visible to the naked eye were obtained by using a continuous large-scale test method, and the basic physical and chemical properties of the supramolecular BPA-HA copolymer were analyzed. The main experimental operation steps are briefly described as follows: (1) The reactor contains 1L of 10mM citric acid-phosphate buffer, 100μM BPA, 30mg·L -1 HA and 2U·mL -1 Yunzhi laccase; (2) Place the reaction system in a dark place at 25°C and pH 5.0, 150r·min -1 After enzymatic oxidative polymerization for 3 hours, immediately add high-concentration hydrochloric acid to acidify the enzymatic reaction solution to pH 1.0; this process can not only quench the active phenol radical intermediate, but also promote the precipitation and precipitation of supramolecular BPA-HA copolymer; (3) The reaction solution quenched by the...

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Abstract

The invention belongs to application microbiology, enzyme catalysis kinetics, water pollution control science and other cross interfusion disciplines, and relates to a method for promoting bisphenol A conversion by laccase-induced copolymerization. According to the method, Trametes versicolor laccase is taken as a biocatalyst, bisphenol A and humic acid are taken as coexisting substrates, and trapping and sealing effects of a long-chain bisphenol A autopolymer on laccase molecules are eliminated through a free radical cross-binding mechanism started by the Trametes versicolor laccase, so that the catalytic activity, stability and durability of the enzyme are maintained, and the activity of the enzyme is improved. The bisphenol A can be simply, quickly and efficiently removed and converted. The coriolus versicolor laccase induces bisphenol A and humic acid to generate supramolecular copolymerization particles which are complex and stable in chemical structure, and the biological toxic effect of bisphenol A monomers on the cherry radishes is thoroughly avoided. The method has the advantages of being controllable in catalysis process, high in removal efficiency, green, low-carbon, energy-saving, environment-friendly and the like, and has great application potential in the aspects of water pollution treatment, global carbon sequestration and the like.

Description

technical field [0001] The invention belongs to the fields of applied microbiology, enzyme catalysis kinetics and water pollution control, and relates to a method for promoting the transformation of bisphenol A by inducing copolymerization with laccase. Background technique [0002] Bisphenol A (BPA), as an important organic plasticizer in the field of industrial manufacturing, is widely used in the production of plastic products containing polymer materials such as polycarbonate and epoxy resin. The BPA in the aquatic ecosystem mainly comes from the discharge of urban sewage and industrial wastewater. BPA exposed to the environment shows typical endocrine disrupting effects on organisms, and can be absorbed and accumulated by the human body through food web transmission, respiration and skin surface contact. , thereby causing public health risks. Currently, high doses of BPA have been detected in natural water bodies, tap water, and even human urine, serum, breast milk, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C12P1/00
Inventor 孙凯刘庆竹刘婕陈美骅司友斌
Owner ANHUI AGRICULTURAL UNIVERSITY
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