Biochemical reaction unit and biochemical reaction device capable of realizing partitioned temperature control

Abstract

The invention relates to biochemical reaction, in particular to a biochemical reaction unit and a biochemical reaction device capable of realizing partitioned temperature control. The biochemical reaction unit sequentially comprises an upper substrate, a middle runner layer and a lower substrate from top to bottom, the upper substrate comprises a liquid inlet and a liquid outlet; the middle flow channel layer comprises a U-shaped preheating flow channel, a connecting flow channel and a biochemical reaction flow channel which are communicated in sequence; wherein the upper substrate, the middle flow channel layer and the lower substrate are sequentially overlapped, so that the liquid inlet is communicated with the preheating flow channel, and the liquid outlet is communicated with the biochemical reaction flow channel. According to the invention, the temperature of the biochemical reaction reagent in the biochemical reaction zone can be accurately controlled, the temperature rising and lowering response speed is fast, the biochemical reaction time caused by the temperature rising and lowering process is shortened, the small temperature gradient at the inlet and the outlet of the biochemical reaction zone is ensured, the temperature consistency of the biochemical reaction zone is ensured, and the biochemical reaction efficiency is improved. Furthermore, the biochemical reaction is kept consistent, and the size and the design difficulty of the heating and cooling device are reduced.

Description

technical field [0001] The invention relates to biochemical reactions, in particular to a biochemical reaction unit and a biochemical reaction device with zoneable temperature control. Background technique [0002] At present, the biochemical reaction unit (Flowcell, which can also be called biochemical reaction pool, flow pool, etc.) is an important carrier in the biochemical reaction. It is necessary to add various biochemical reaction reagents to the biochemical reaction unit in a logical order at a specific temperature to occur smoothly. Relevant biochemical reactions, and different biochemical reaction reagents have different temperature requirements or optimal temperature ranges when biochemical reactions occur in the biochemical reaction unit. [0003] Some prior art schemes use water baths to soak and heat the reagent tubes or tanks. The water baths provide heat sources to the biochemical reaction reagents to heat the biochemical reaction reagents or keep the biochem...

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Problems solved by technology

[0003] Some prior art schemes use water baths to soak and heat the reagent tubes or tanks. The water baths provide heat sources to the biochemical reaction reagents to heat the biochemical reaction reagents or keep the biochemical reaction reagents at a constant temperature. However, such devices are often bulky and expensive. High, slow heating and cooling response

[0004] There are also some existing technical schemes that use a thermoelectric cooler (Thermo Electric Cooler, TEC) to form a cavity to heat or cool down the reagent tube or tank, but it is still not sensitive and accurate enough for the temperature control of the biochemical reaction reagent when it enters the biochemical reaction unit , there is still a certain temperature gradient at the entrance and exit of the biochemical reaction zone of the biochemical reaction unit, resulting in the inhomogeneity of the temperature in the biochemical reaction zone, which may lead to different results of biochemical reactions in the same biochemical reaction unit

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