Preparation method of active probiotics
A technology of active probiotics and cocci, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of single taste and nutrient composition, low content of active probiotics, poor product function effect, etc., to achieve the taste Cooling, increased activity, uniform color effect
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Embodiment 1
[0034] The invention provides a preparation method of active probiotics, comprising the following steps:
[0035] S1: selection of raw materials, one of Lactobacillus, one of Gram-positive coccus and one of Bifidobacterium are selected as probiotic strains respectively;
[0036] S2: Activation, preserve and inoculate the above-mentioned lactobacillus into the lactobacillus culture medium, activate at 30°C for 12 hours, preserve and inoculate the above-mentioned gram-positive cocci into the gram-positive cocci medium, and inoculate at 28 Under the condition of ℃, activate for 12h, the bifidobacteria mentioned above are preserved and inoculated into the bifidobacterium medium, and activated under the condition of 30℃ for 12h;
[0037]S3: Inoculate, inoculate the activators of Lactobacillus, Gram-positive cocci and Bifidobacterium in S2 into seed tanks with 2% inoculation amount, control the temperature at 25°C, and culture for 24 hours;
[0038] S4: Preparation of culture mediu...
Embodiment 2
[0049] A preparation method of active probiotics, comprising the following steps:
[0050] S1: selection of raw materials, one of Lactobacillus, one of Gram-positive coccus and one of Bifidobacterium are selected as probiotic strains respectively;
[0051] S2: Activation. Preserve and inoculate the above-mentioned Lactobacillus into the Lactobacillus culture medium, activate at 30-36°C for 18 hours, preserve and inoculate the above-mentioned Gram-positive cocci into the Gram-positive cocci culture medium, Activated at 30°C for 18 hours, preserved and inoculated the bifidobacterium in the above-mentioned bifidobacteria culture medium, and activated at 32°C for 18 hours;
[0052] S3: Inoculate, inoculate the activators of Lactobacillus, Gram-positive cocci and Bifidobacterium in S2 into the seed tank with an inoculation amount of 3%, control the temperature at 30°C, and culture for 30 hours;
[0053] S4: Preparation of culture medium: 1% glucose, 1.5% soluble starch, 1% peptone...
Embodiment 3
[0064] A preparation method of active probiotics, comprising the following steps:
[0065] S1: selection of raw materials, one of Lactobacillus, one of Gram-positive coccus and one of Bifidobacterium are selected as probiotic strains respectively;
[0066] S2: Activation, preserve and inoculate the above-mentioned lactobacilli into the lactobacillus culture medium, activate at 36°C for 24 hours, preserve and inoculate the above-mentioned gram-positive cocci into the gram-positive cocci medium, and inoculate them at 32°C Under the condition of ℃, it is activated for 24 hours. The bifidobacteria mentioned above are preserved and inoculated into the bifidobacterium medium, and activated under the condition of 34 degrees Celsius for 24 hours;
[0067] S3: Inoculate, inoculate the activators of Lactobacillus, Gram-positive cocci and Bifidobacterium in S2 into the seed tank with 4% inoculation amount, control the temperature at 35°C, and culture for 36 hours;
[0068] S4: Preparati...
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