Cell culture plate and use method thereof

Abstract

The invention discloses a cell culture plate and a use method thereof, and belongs to the technical field of cell culture.The cell culture plate has the advantages that a culture plate body can be semi-closed by the aid of an upper cover, temperature stability and balanced humidity environment in the culture plate body can be improved by the aid of an annular groove, and a magnetic field is applied by an electromagnet; the magnetic repulsion sealing ball on the upper cover is forced to leave the sphere-like hole to be communicated with the outside, the magnetic repulsion sealing ball pulls the oxygen release driving ball to deform, compress and expand towards the side edge through the upper pull wire, so that the ventilation branch cover is forced to separate and stir air flow, then air enters from the sphere-like hole to supplement oxygen, and meanwhile generated carbon dioxide can be discharged along with air flow. And then the rest electromagnets are sequentially controlled to open the plurality of sphere-like holes, so that the cell culture environment in the culture plate body is relatively stable, particularly the temperature and the humidity are relatively uniform, and the fringe effect of cell culture is obviously improved and even eliminated.

Description

technical field [0001] The invention relates to the technical field of cell culture, and more specifically relates to a cell culture plate and a method for using the same. Background technique [0002] Cell culture plate is a kind of biological tool. The edge effect in cell culture means that in the cell culture using multi-well plate, the cells in the peripheral wells will be concentrated on the outside of the well. This phenomenon is commonly used in experiments. 96 It is very obvious in the orifice plate. The number of edge holes accounts for 1 / 3 of the total, which means that the impact caused by this error is quite large. [0003] Since the growth of most cells will be inhibited by surrounding contact cells, the edge effect causes the average growth rate of cells in edge wells to be slower than that in other wells, thus introducing systematic errors in the experimental process. In many large-scale drug screening experiments, people even discard the experimental result...

AI Technical Summary

Problems solved by technology

The defect of this method is that for the fragile cells, the reduction of the external environment temperature will lead to the generation of cell stress response, thereby changing the character of the cells, especially the primary cultured cells can not tolerate such harsh living conditions, so the cell growth state will be abnormal. Good, apoptosis or even death

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