Loquat primary pulp beer yeast fermentation method
A technology of brewer's yeast and fermentation method, applied in the field of loquat puree brewer's yeast fermentation, can solve the problems of poor fermentation performance of yeast, not suitable for loquat beer fermentation, poor activity, etc., and achieves stable alcohol production capacity and good passage stability. Effect
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Embodiment 1
[0035] Please refer to the attached figure 2 , S1: medium selection
[0036] S1.1. Enrichment medium: Add 4 times of water to the malt extract powder, heat it for saccharification at 60°C, test with iodine solution until the sugar is over, and adjust the sugar to 10Bx with sucrose;
[0037] S1.2, slant preservation medium: containing glucose 20g / L, peptone 20g / L, yeast extract 10g / L, agar 20g / L, sterilized at 121°C for 15min;
[0038] S1.3, Duchenne tube fermentation medium: containing glucose 20g / L, peptone 20g / L, yeast extract 10g / L, sterilized at 121°C for 15min;
[0039] S1.4, TTC chromogenic medium: containing glucose 6g / L, agar 15g / L, TTC 0.4g / L, peptone 20g / L, yeast extract 10g / L, sterilized at 121°C for 15min.
Embodiment 2
[0041] S2: yeast enrichment culture
[0042] Select mature and clean loquat pulp for beating, add sulfur dioxide 100mg / L, pectinase 100mg / L, treat at 30°C for 3 hours, use sucrose to adjust the sugar degree to 21Brix, natural pH, add 300mL beating liquid to a 500mL sterile Erlenmeyer flask, and ferment at 28°C until No air bubbles are produced, take 10mL of loquat pulp natural fermentation liquid and dilute it to 10 with sterile water -1 , and then gradually diluted to 10 -6 After shaking for 5 minutes, draw 0.1mL of the supernatant and spread it on the solid medium of wort agar, keep the temperature at 28°C for 48 hours, after the strain grows, pick a single colony with a smooth and creamy surface and a wine aroma, and separate it by streaking 2 -3 times, and finally a single typical colony was transplanted into the slant preservation medium for preservation;
[0043]The color of the isolated yeast colony is white or milky white, the color in the middle is the same as the c...
Embodiment 3
[0045] Please refer to the attached image 3 , S3: yeast screening
[0046] S3.1, first-level screening: Inoculate each isolated bacterial strain in the slant preservation medium, and cover a layer of slant preservation medium containing 0.5% TTC chromogenic agent on the grown colonies for 24 hours to form a double-layer culture medium. Yeast colonies with different alcohol concentrations will display various colors, dark red is the colony of high alcohol concentration, from which yeast strains with good characteristics and dark color are selected;
[0047] After 24 hours of cultivation and 3 hours of chromogen development, 12 yeast strains were red, and 48 yeast strains were dark red, and 60 yeast strains with strong wine-producing ability were used for secondary screening;
[0048] S3.2. Secondary screening: Introduce the first-stage screening strains into the Dunaliella tube fermentation medium containing Dunaliella tubules, and screen according to the gas production time ...
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