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Method for measuring lectin-binding substance, kit for measuring lectin-binding substance, and block-labeled lectin used in said method and kit

An assay method and lectin technology, which is applied in the field of block-labeled lectin, can solve the problems of weak affinity reaction and difficulty in high-sensitivity assay

Pending Publication Date: 2022-04-08
FUJIREBIO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the method of measuring lectin-binding substances has the following problem: Even if only lectin is used, the affinity between lectin and sugar chain is weaker than that of antigen-antibody reaction, so the high-level method such as CLEIA method (chemiluminescence enzyme immunoassay) Sensitivity determination is difficult

Method used

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  • Method for measuring lectin-binding substance, kit for measuring lectin-binding substance, and block-labeled lectin used in said method and kit
  • Method for measuring lectin-binding substance, kit for measuring lectin-binding substance, and block-labeled lectin used in said method and kit
  • Method for measuring lectin-binding substance, kit for measuring lectin-binding substance, and block-labeled lectin used in said method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0135] Determination of alpha-fetoprotein L3 fraction (AFP-L3) using block-labeled lectin and labeled lectin

[0136] (1) Preparation of hydrazine dextran

[0137] 240.0 mg of dextran with a molecular weight of 250K (manufactured by CarboMer) was added to 4.8 mL of 0.1 M phosphate buffer (pH 7.0), and stirred and dissolved in the dark at 25° C. for 30 minutes. Next, add 150mM NaIO 4 2.664 mL and 0.536 mL of ion-exchanged water were stirred in the dark at 25°C for 30 minutes. Using a PD-10 column (manufactured by GE Healthcare, Sephadex G-25 packed column), buffer exchange was performed with 0.1 M sodium phosphate buffer (pH 6.0) to obtain 20.0 mL of a solution. Add 5.04g of NH 2 NH 2 • HCl, stirred in the dark at 25°C for 2 hours. 800 mg of DMAB (dimethylamine borane) was added, and it stirred in the dark at 25 degreeC for 2 hours more. After performing dialysis with 4 L of ion-exchanged water in the dark using an RC50K (regenerated cellulose with a molecular weight of ...

Embodiment 2

[0159] Measurement of PSA derived from prostate cancer cells using block-labeled lectin and labeled lectin 1

[0160] (1) Use AAL to prepare block-labeled lectins

[0161] Except for using Dictyosporum amaranthus agglutinin (AAL; manufactured by VECTOR Co., Ltd.) instead of lentil agglutinin (LCA), it was carried out in the same manner as in Example 1 (1) to (5) to obtain 87.8 μg / mL 2.0 mL of fragmented labeled lectin 2 (dextran-enzyme-AAL conjugate) solution.

[0162] (2) Prepare labeled lectin using AAL

[0163] Except that the lentil agglutinin (LCA) was replaced with Dictyospermia aurantii agglutinin (AAL; manufactured by VECTOR Co., Ltd.), 34.4 μg / mL of labeled lectin 2 was obtained in the same manner as in (6) of Example 1. (ALP-AAL conjugate) solution 2.0 mL.

[0164] (3) Measurement of PSA derived from cancer cells

[0165] Magnetic particles (manufactured by Fuji Xerbio Co., Ltd.) and anti-PSA monoclonal antibody F(ab') 2 Fragments (manufactured by Fuji Xerbio Co...

Embodiment 3

[0172] Assay of AFP-L3 Using Multiple Blocked-Tagged Lectin Mixtures

[0173] (1) Preparation of block-labeled lectin

[0174] Except using dextran with a molecular weight of 500K (manufactured by Fluka Corporation) or dextran with a molecular weight of 70K (manufactured by TCI Corporation) as dextran, the same procedure as in (1) to (5) of Example 1 was performed to prepare each Block-tagged lectins. The block-labeled lectin using dextran with a molecular weight of 500K is referred to as "block-labeled lectin 3 (500K)", and the block-labeled lectin using dextran with a molecular weight of 70K is referred to as "block UL-tagged lectin 4 (70K)".

[0175] (2) Determination of AFP-L3 1

[0176]The composition of the particle dilution solution was set to 50mM Tris, 150mM NaCl, 2mM EDTA·2Na, 0.5mg / mL dextran sodium sulfate 5000, 30μg / mL mouse KLG, 1.0% BSA, 0.005% Antiform SI, 0.1% ProClin 300 , pH 7.2, except that, proceed in the same manner as in (7) of Example 1 to prepare a...

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Abstract

A method for measuring a lectin-binding substance in a sample, said method comprising: a measurement step in which a block-labeled lectin is brought into contact with the sample to measure a lectin-binding substance in the sample; the block-labeled lectin comprises a water-soluble carrier containing a first water-soluble polymer, and a marker and lectin immobilized on the water-soluble carrier.

Description

technical field [0001] The present invention relates to a method for measuring a lectin-binding substance, a kit for measuring a lectin-binding substance, and a block-labeled lectin used therefor. Background technique [0002] In recent years, methods for measuring sugar chains such as glycoproteins, glycolipids, and free sugar chains or substances having sugar chains have been extensively studied as markers for monitoring malignant diseases such as tumor markers. Among them, from the viewpoint of being able to measure not only the quantitative change of the above-mentioned test substance but also the qualitative change caused by the sugar chain change, the measurement method using a lectin that binds to the sugar chain portion of the test substance, and the measurement method and Attention has been drawn to a measurement method using a measurement combination of an antibody of the analyte. As the test substance (lectin-binding substance) that can be measured by the above-m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/574G01N33/53
CPCG01N33/543G01N2333/4724G01N33/57484G01N2333/471
Inventor 西井友教冈田和训石川直树饭田久美子八木慎太郎青柳克己
Owner FUJIREBIO CO LTD
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