Mycelium or thallus, application of mycelium or thallus and method for breeding bacterial strain by using mycelium or thallus

A technology of mycelium and thalline, which is applied in the breeding of strains resistant to their own metabolites, mycelium or thalline and its application fields, which can solve the problem that the production level of Zhongshengmycin fermentation cannot be further improved and the market supply is limited Issues such as demand extension and expansion of use, and the content of Zhongshengmycin master drug products cannot be further increased

Pending Publication Date: 2022-04-08
福建凯立生物制品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Zhongshengmycin has been produced by industrial fermentation for 20 years. The metabolites of mycin-producing bacteria, such as glucose effect, self-secondary metabolite Zhongshengmycin, and many other single-factor and multi-factor repression and feedback inhibition regulation make the fermentation production level of Zhongshengmycin unable to be further improved, and the biological titer Hovering between 8000μ / ml-12000μ / ml, the content of Zhongshengmycin master drug products cannot be further increased, which limits the market supply and demand of biological pesticide Zhongshengmycin master drugs and their preparations and their comprehensive integration in large areas The application of flight defense and other fields is extended and expanded

Method used

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  • Mycelium or thallus, application of mycelium or thallus and method for breeding bacterial strain by using mycelium or thallus
  • Mycelium or thallus, application of mycelium or thallus and method for breeding bacterial strain by using mycelium or thallus
  • Mycelium or thallus, application of mycelium or thallus and method for breeding bacterial strain by using mycelium or thallus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 The mutagenic strain (UV-3) of Streptomyces lilacinus gray Hainan variant strain (preservation number is CGMCC No.1026) is used as an industrial production strain to carry out Zhongshengmycin industrial fermentation production

[0084] Strain UV-3 is the Streptomyces lavenderus Hainan variety obtained by the applicant through mutagenesis with the preservation number of CGMCC No. 1026 and has been applied to the industrial fermentation production of Zhongshengmycin. mutagenic strains.

[0085] Put UV-3 at 40m 3 Industrialized fermentation was carried out in the fermenter (according to the aforementioned "fermentation medium formula and fermentation culture conditions", the fermentation culture period was 5 days) and the fermentation broth at different stages in the fermentation process was collected.

[0086] The above-mentioned industrialized fermentation broth is sampled and collected according to the requirements of aseptic sampling from the "0" hour after ...

Embodiment 2

[0128] The preparation of embodiment 2 target departure bacterial strains

[0129] 1. According to the measurement result of the biological potency of the fermentation broth sample, select the fermentation broth sample collected from the fermentation of Example 1 to 48 hours (the end stage in the rapid growth accumulation stage).

[0130] 2. Take out the triangular flask containing the Zhongshengmycin fermentation liquid sample stored in the refrigerator at 4°C that is not contaminated by other microorganisms, sterilize the outer surface of the triangular flask with ultraviolet radiation and sterilize the outer surface of the triangular flask with 75% alcohol cotton. After all-round wiping and disinfection, move it into the ultra-clean workbench in the aseptic operation room to perform the entire aseptic operation;

[0131] 3. In a sterile room, shake the above-mentioned Zhongshengmycin fermentation liquid evenly at room temperature, draw 100ml into a 250ml triangular flask wi...

Embodiment 3

[0133] Embodiment 3 Preparation of Tolerant Substrate Factors

[0134] 1. According to the measurement result of the biological potency of the fermentation broth sample, select the fermentation broth sample collected from the fermentation of Example 1 to 120 hours (the last stage of growth stagnation).

[0135] 2. Take out the triangular flask containing the Zhongshengmycin fermentation liquid sample stored in the refrigerator at 4°C that has not been contaminated by other microorganisms, disinfect the outer surface of the triangular flask and sterilize the outer wall of the triangular flask with 75% alcohol cotton. After azimuth wiping and disinfection, move it into the ultra-clean workbench in the aseptic operation room, and carry out the whole process of aseptic operation;

[0136] 3. Shake the above-mentioned Zhongshengmycin fermentation liquid evenly, draw 500ml into a 1000ml Erlenmeyer flask with 50 glass balls built in (the Erlenmeyer flask and its contents have been st...

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Abstract

The invention discloses a mycelium or thallus, application of the mycelium or thallus and a method for breeding a bacterial strain by using the mycelium or thallus. The mycelia or thalli are obtained from fermentation liquor at the tail section of a secondary metabolite production rapid growth and accumulation stage in a strain fermentation production process. When the obtained mycelium or thallus is applied to breeding of a streptomyces lavendulae strain resistant to metabolite, a Zhongshengmycin high-yield strain is screened, the industrial fermentation production level of the high-yield strain can be greatly improved, the industrial production cost of Zhongshengmycin is greatly reduced, and the method is suitable for industrial production of Zhongshengmycin. A foundation is laid for the market demand of Zhongshengmycin in biopesticide, and the extended use in the fields of green ecological agriculture large-range large-scale flight prevention pesticide and the like is met.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a mycelium or thalline and its application, and a method for breeding strains tolerant to their own metabolites. Background technique [0002] According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), in the process of agricultural and forestry production in the world; the annual loss caused by diseases, insect pests and weeds is more than one-third. About 3 million cases of chemical pesticide poisoning occur every year in the world. In developing countries, there are about 1,650 mass poisoning incidents worldwide each year caused by eating pesticide-contaminated food. Therefore, it has become an inevitable trend and a global consensus to vigorously develop biopesticides that are safe, low-toxic and non-toxic, low-accumulating and non-accumulating, and do not pollute the environment. [0003] Zhongshengmycin (Chemical name: 1-N gluc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00C12P19/28C12R1/56
Inventor 陈俊升陈灼湖刘炳珠刘茂花林小敏郑毅伟
Owner 福建凯立生物制品有限公司
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