Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of cationic starch-based glutathione peroxidase

A technology of glutathione peroxide and cationic starch, applied in the field of starch modification, can solve the problems of restricting industrialization development, high cost, limited and other problems, so as to improve the substrate binding ability, improve the catalytic activity, and expand the scope of application Effect

Active Publication Date: 2022-04-01
BEIBU GULF UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, it is difficult for most small molecule bionic GPx to realize the overall simulation of GPx synergistic catalytic process
Supramolecular biomimetic GPx can usually realize the synergistic catalysis of various catalytic primitives, but it can only be realized under specific conditions, which has certain limitations in application.
The structure and catalytic activity of biomimetic GPx with protein backbone can be compared with natural GPx, but the limited raw materials and high cost limit its industrialization development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of cationic starch-based glutathione peroxidase
  • Preparation method of cationic starch-based glutathione peroxidase
  • Preparation method of cationic starch-based glutathione peroxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of preparation method of cationic starch-based glutathione peroxidase (preparation route sees figure 1 ), including the following steps:

[0030] (1) 30g of cassava starch (Cassava starch) was added into the mixed solvent of absolute ethanol / water and stirred to prepare starch emulsion. Octenyl succinic anhydride (3.5% of starch dry weight) was dissolved in 5ml of ethanol, and it was slowly added dropwise into the flask of starch emulsion. During the dropwise reaction, the pH of the reaction system was maintained at about 8.5. React at 40°C for 6 hours. After the reaction, deionized water, 75% ethanol and absolute ethanol are used to wash, filter, and dry to obtain OSAstarch.

[0031] (2) 123.90 mg of selenium and 178.10 mg of sodium borohydride were added to 20 mL of deionized water, and a stock solution of sodium selenium hydride (NaSeH) was prepared under a nitrogen atmosphere. 5.0g of OSA starch was added to 80mL of absolute ethanol (the volume ratio of ab...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of starch modification, in particular to a preparation method of cationic starch-based glutathione peroxidase. The invention relates to a preparation method of cationic starch-based glutathione peroxidase. The preparation method comprises the following steps: (1) preparing OSA (Octenyl Succinic Acid) starch ester; (2) preparation of selenium starch (Se-Starch); and (3) preparation of the cationic Se-Starch (Cation Se-Starch). The cationic selenized starch can simulate a catalytic center, a hydrophobic microenvironment and a substrate recognition site of natural glutathione peroxidase (GPx), and meanwhile, the effective matching and synergistic effect of three catalytic elements of the catalytic center, the hydrophobic microenvironment and the recognition site plays a positive role in improving the antioxidant catalytic activity of the cationic selenized starch.

Description

technical field [0001] The invention relates to the technical field of starch modification, in particular to a preparation method of cationic starch-based glutathione peroxidase. Background technique [0002] Glutathione peroxidase (GPx) is an important antioxidant enzyme that can scavenge reactive oxygen species and protect cells from oxidative damage. However, due to the limited source of natural GPx and its poor stability, its application development is limited, so the design and synthesis of highly efficient GPx antioxidant enzyme mimics has attracted widespread attention in the academic community. Based on the understanding of the structure and characteristics of GPx, studies have shown that catalytic centers, hydrophobic microenvironments and recognition sites are important catalytic units for the construction of efficient biomimetic GPx, and their synergistic catalysis plays a very important role in improving the activity of biomimetic GPx . [0003] The skeleton-ba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B31/16C08B31/04C08B31/12C12N9/08
Inventor 张瑞瑞石成郑韵英尹艳镇梁兴唐张艳军
Owner BEIBU GULF UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com