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A method for improving the efficiency of aspergillus producing organic acids

An organic acid and Aspergillus technology, applied in the field of bioengineering, can solve problems such as patent publications that have not yet been found, and achieve the effects of improving biosynthesis ability, shortening fermentation cycle, and increasing yield

Active Publication Date: 2022-06-17
南京昊禾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • A method for improving the efficiency of aspergillus producing organic acids
  • A method for improving the efficiency of aspergillus producing organic acids
  • A method for improving the efficiency of aspergillus producing organic acids

Examples

Experimental program
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Effect test

preparation example Construction

[0048] The preparation of described aspergillus seed liquid is as follows:

[0049] 2~4% glucose, 0.01~0.02% metal ions, 1~2% nitrogen source, sterilized at 115°C for 30 min, inoculated with Aspergillus spores 1.5×10 6 cells / mL, incubate at 30°C and 200 rpm for 14-20 h;

[0050] Wherein, the above percentage is the final mass concentration.

[0051] Preferably, the volume ratio of the waste cordyceps fermentation broth and fermentation medium is 1-3:5.

[0052] Preferably, the discarded cordyceps fermented liquid is one or more mixed liquids of cordyceps militaris fermented liquid, Xinjiang cordyceps fermented liquid, and Liangshan cordyceps fermented liquid.

[0053]Preferably, the volume ratio of the waste cordyceps fermentation liquid and the fermentation medium is: 1-3:5, wherein the waste cordyceps fermentation liquid is a single species of cordyceps fermentation liquid or a mixed liquid of two or more cordyceps fermentation liquids;

[0054] Or, when adding two kinds ...

Embodiment 1

[0069] Example 1 Method for improving the efficiency of malic acid production by Aspergillus by exogenously adding waste cordyceps fermentation broth

[0070] The operation steps of multiple experimental groups are basically the same, and the difference is only that the type and consumption of the waste cordyceps fermentation broth are different. For details, see Table 1:

[0071]

[0072] The fermentation time (the time from residual sugar to single digits) in experimental groups 1-3 was recorded, the changes in glucose content during fermentation were detected, and the organic acid production efficiency was calculated.

[0073] No-addition group A: This group is the control group of experimental groups 1-3. The same method as the experimental group is used to ferment and produce organic acids, except that the fermentation medium does not add waste cordyceps fermented liquid.

[0074] The organic acid production efficiency of experimental groups 1-3 and control group A was...

Embodiment 2

[0078] Example 2 The method for improving the efficiency of citric acid production by Aspergillus by adding waste cordyceps fermentation broth

[0079] The operation steps of multiple experimental groups are basically the same, and the difference is only that the type and consumption of waste cordyceps fermentation broth are different. For details, see Table 2:

[0080]

[0081] Record the time of fermentation and cultivation in specific experimental groups 4-6 (the time from the amount of residual sugar to single digit), detect the change of glucose content during fermentation and cultivation, and calculate the production efficiency of organic acid.

[0082] No-addition group B: This group is the control group of experimental groups 4-6. The same method as the experimental group is used to ferment and produce organic acids, except that the fermentation medium does not add waste cordyceps fermented liquid.

[0083] The organic acid production efficiency of experimental grou...

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Abstract

The invention discloses a method for improving the efficiency of aspergillus producing organic acids. In the method, after the aspergillus fermentation medium is sterilized, discarded cordyceps fermentation broth is added to the aspergillus fermentation medium to obtain an improved aspergillus fermentation medium. Inoculate 10% Aspergillus seed liquid into the improved Aspergillus fermentation medium, mix well and culture on a shaking table to obtain organic acids produced by Aspergillus. The method of the present invention adopts the method of non-genetic engineering, and by adding waste Cordyceps fermented liquid from an external source, the nutrient composition of the Aspergillus fermentation medium is changed, the intracellular synthesis pathway is strengthened, the production intensity of the organic acid is improved, the fermentation period is shortened, and the organic acid is increased. Increase production efficiency and reduce fermentation cost. The invention improves the problems of long period and low efficiency in the production of organic acid by aspergillus fermentation in the prior technical conditions through simple external source addition.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for improving the organic acid production efficiency of Aspergillus. Background technique [0002] Organic acids refer to acidic organic compounds (except amino acids) containing carboxyl groups that are widely present in organisms, including malic acid, citric acid and fumaric acid. These organic acids are mostly involved in the life process of animal and plant metabolism, can be used as intermediate products of metabolism, or have significant biological activity, and are important raw materials for organic synthesis and development of pharmaceutical products. Great market application value. [0003] Most organic acids are intermediates in the metabolic pathways of microorganisms, and the process of producing organic acids by fermentation of microorganisms from renewable biomass resources is very promising. Aspergillus is regarded as an ideal microorganism for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/40C12R1/69C12R1/685
CPCC12P7/40
Inventor 黄和刘浩徐晴
Owner 南京昊禾生物科技有限公司
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