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Natural anti-stimulation repair factor and preparation method thereof

An anti-irritant and natural technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of skin cell toxicity, etc., and achieve the effects of fast growth, promoting expression, and effective cells

Inactive Publication Date: 2019-08-16
江西登云健康美业互联有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the existing anti-ultraviolet stimulation natural repair factor is toxic to skin cells, and to provide a kind of Brassica plant, effective and safe anti-ultraviolet stimulation natural repair factor and its preparation method

Method used

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  • Natural anti-stimulation repair factor and preparation method thereof
  • Natural anti-stimulation repair factor and preparation method thereof
  • Natural anti-stimulation repair factor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1.1 Cultivation of Chinese cabbage test tube root

[0040] Specifically include the following:

[0041] (1) Leaf disk transformation

[0042] ① Sterilize the cabbage seeds with 70% ethanol for 1 to 3 minutes, then treat them with 1% NaClO for 15 minutes, and wash them with sterile water three times. The treated seeds were cultured on MS solid medium at a culture temperature of 25° C., with a photoperiod of 8 h dark / 16 h light.

[0043] ②Cultivate Agrobacterium rhizogenes (Ri15834) at 27°C for 3 days and dilute to OD 600 0.3 to 0.5.

[0044] ③ After the cabbage seeds have been cultivated for about three weeks, take the leaves, cut out the size of 5mm × 5mm, then mix with Agrobacterium rhizogenes, stir, and place for 20 minutes, then put the leaves in the solid medium B5M ( Containing B5 Gamborg 1.55g / L, agar 7g / L, inositol 250mg / L and 30g / L sucrose, pH 5.7) for 3 days.

[0045] ④ On the fourth day, the explants were washed with sterile water and dried on filter pape...

Embodiment 2

[0115] Example 2 Effect of the mixture of cell wall extract and ethanol extract on melanin production and protein expression in basement membrane

[0116] 2.1 Effect of the mixture of cell wall extract and ethanol extract on melanin production

[0117] Murine melanoma cells (B16F1) were treated with a mixture of the two extracts at a concentration of 0.0002% (2 μg / ml) for 72 hours in the presence of IBMX.

[0118] From Figure 9 As can be seen in , this treatment significantly reduced the melanin content: a reduction of about 15%. and figure 2 The results were similar when treated with the same concentration of cell wall extract as shown in . This result indicated that the mixture of the two extracts retained only the melanin-reducing ability of the cell wall extract and that the presence of the ethanol extract did not interfere with this activity.

[0119] 2.2 The effect of the mixture of cell wall extract and ethanol extract on the expression of basement membrane consti...

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Abstract

The invention relates to a natural anti-stimulation repair factor and a preparation method thereof. The natural anti-stimulation repair factor is a plant root extract. A plant is transformed by agrobacterium rhizogenes via a leaf disc method, test-tube roots are obtained through in vitro culture, and then the repair factor is obtained from the test-tube roots. The repair factor can be applied in cosmetics for repairing skin.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a natural anti-irritation repair factor and a preparation method thereof. Background technique [0002] Ultraviolet radiation can cause a series of physiological changes such as skin pigmentation, elasticity changes, dryness and roughness, and wrinkle formation. [0003] Hyperpigmentation refers to dark pigmentation. For healthy skin, there are various factors that affect its color, and melanin is one of them. Melanin is located in the basal cell layer of the epidermis and accumulates in mature melanosomes, which are then transferred to adjacent keratinocytes, giving the epidermis its typical brown color. Although the concentration of melanocytes is similar in human skin, the activity of melanocytes varies between different ethnic groups. When skin is exposed to the sun, UV rays damage the epidermis, causing keratinocytes to release the polypeptide - pro-opioid melanocortin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/99A61Q19/00A61Q17/04
CPCA61K8/99A61K2800/592A61K2800/782A61Q17/04A61Q19/00A61K8/9789
Inventor 林敏
Owner 江西登云健康美业互联有限公司
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