Method for fixed-point quantitative detection of cancer marker based on micro-fluidic chip

Abstract

The invention discloses a method for fixed-point quantitative detection of a cancer marker based on a micro-fluidic chip, a PDMS channel is composed of six concentration gradient to-be-detected substance channels formed by bifurcating after two inlets, an S-shaped curve channel is adopted for uniform mixing, and in order to reduce dead volume caused by a right angle, an arc is adopted at the joint; the micro-fluidic chip is formed by bonding PDMS and glass, and the PDMS is provided with a micron-sized channel, so that the reagent cost is greatly reduced, the integration degree is high, and the micro-fluidic chip has the potential of automatic batch production and portable detection; a PDMS brush printing method is adopted for modification of amino groups of the microfluidic channel, and brushes are designed at unequal intervals, so that fixed-point modification, comparison and observation are facilitated; the biotin-modified aptamer can be connected with streptavidin, the fluorescence brightness on the aptamer can reflect the concentration of the connected aptamer, the aptamer is quenched by a BHQ fluorescence quenching agent along with fluorescence, then a to-be-detected substance combined with the aptamer is added, and the concentration of the to-be-detected substance is judged according to the recovery degree of the fluorescence intensity. And the detection of'on-off-on 'is realized. The method can meet the requirements of simultaneously detecting various cancer markers and realizing trace and accurate detection of the cancer markers.

Description

technical field [0001] The invention belongs to the field of cancer marker detection, and in particular relates to a new type of microfluidic chip and the layer-by-layer modification of the chip, and the use of the modified chip to quantitatively detect various cancer markers by using fluorescence intensity to realize the combination of various cancer markers. method of detection. Background technique [0002] Serum levels of CA125, HE4, and CEA are closely related to the occurrence and development of epithelial ovarian cancer. Joint detection of CA125, HE4, and CEA is of great significance for early diagnosis, disease development monitoring, and prognosis evaluation of epithelial ovarian cancer [1] . In clinical trials, cancer marker levels are often measured radiologically [2] and enzyme immunoassay [3] to measure. These traditional analyzes are time-consuming and require laboratories with special equipment. In recent decades, several methods for the detection of canc...

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for fixed-point quantitative detection of cancer markers based on a microfluidic chip, which solves the problems of time-consuming analysis and low precision in the traditional method for detecting cancer markers in the prior art

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