Microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticide and preparation method thereof

Abstract

The invention belongs to the technical field of microbial agents, and discloses a microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides and a preparation method, and the microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides comprises bacillus subtilis GSMSC30266. The microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides provided by the invention has the functions of efficiently degrading various organophosphorus pesticides, and can promote plant growth, improve soil environment and inhibit the growth of various phytopathogens at the same time. The bacillus subtilis provided by the invention is obtained through 12C6 + ion beam irradiation breeding, has the characteristic of efficiently degrading organophosphorus pesticides, not only can efficiently degrade the organophosphorus pesticides phoxim and omethoate, but also has a relatively good degradation effect on phorate, parathion, chlorpyrifos and trichlorfon, and the degradation rate is 65% or above. The bacillus subtilis microbial agent product disclosed by the invention is simple in production process and has very high application and popularization values.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a microbial bacterial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides and a preparation method. Background technique [0002] At present, the pollution caused by pesticides to the soil environment, water environment, atmospheric environment and the entire ecosystem is becoming more and more serious, especially the pesticide pollution leads to the accumulation of organisms, which has caused serious harm to human health. More than 80% of organophosphorus pesticides are highly toxic, such as phorate, parathion, methamidophos, etc. In 2003, the Ministry of Agriculture canceled the registration of 5 highly toxic organophosphorus pesticides such as parathion and methamidophos. In 2013, the Ministry of Agriculture promoted the use of chlorpyrifos on vegetables. Low toxicity pesticides such as Baichong are still allo...

AI Technical Summary

Problems solved by technology

[0006] (1) At present, the research on microorganisms degrading organophosphorus pesticides mainly focuses on the establishment of pesticide degradation pathways, mechanisms, metabolites and genetically engineered bacteria, and there are few studies on the functions of microorganisms themselves

[0007] (2) In the prior art, there is no report on the scheme of verifying whether Bacillus subtilis can effectively degrade the organophosphorus pesticides phorate, parathion, chlorpyrifos and trichlorfon

[0008] The difficulty in solving the above problems and defects is: it is difficult to simultaneously degrade multiple organophosphorus pesticides through a single microbial strain, and the degradation rate is above 65%;

[0009] The significance of solving the above problems and defects is: pesticides have made great contributions to the control of crop diseases and insect pests, but pesticide residues have brought serious harm to the environment and human health

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