Biological cornea prepared from swimming bladder as well as preparation method and application of biological cornea
A swim bladder and cornea technology, applied in the field of medical materials, can solve the problems of destroying the original structure and composition of the matrix, affecting the transparency and mechanical strength, and the transparency of inflammatory reactions, so as to improve light transmission, improve or restore vision, and biocompatibility sex good effect
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Embodiment 1
[0029] A method for preparing a biocornea by using swim bladders. The swim bladders of fresh Spanish mackerel are degreased, desugared, and freeze-thawed after removing impurities, and then added with decellularization solution to obtain the acellular matrix of the swim bladders. The artificial biological cornea was obtained by reconstituting the alcohol-water mixture of the photomodulator.
[0030] Concrete preparation method is:
[0031] Add a mixed solution of dichloromethane and ethanol after removing impurities from the fresh mackerel swim bladder, the volume ratio of dichloromethane and ethanol is 0.1:1, the speed of the shaker is controlled at 50 r / min, and the degreasing treatment is 0.5h;
[0032] Add 1% sodium chloride solution in the swim bladder after degreasing treatment, control the shaker speed to 50r / min, and desugar for 0.5h;
[0033] After the desugar treatment, the swim bladder was frozen and thawed, and the sodium lauryl sulfate solution with a mass percen...
Embodiment 2
[0037]A method for preparing a biocornea by using swim bladders. The swim bladders of fresh silver carp are degreased, desugared and freeze-thawed after removing impurities, and then decellularized liquid is added to obtain the acellular matrix of the swim bladders. The artificial biological cornea was obtained by reconstituting the alcohol-water mixture of the photomodulator.
[0038] Concrete preparation method is:
[0039] Add degreasing liquid after the swim bladder of fresh silver carp removes impurities, and described degreasing liquid is the mixed solution of A liquid and B liquid, and described A liquid is the mixed liquid of carbon tetrachloride and trichloroethylene, and described B liquid is methanol and ethanol The mixed solution, the volume ratio of A liquid and B liquid is 10:1, the speed of the control shaker is 150 r / min, and the degreasing treatment is 2h;
[0040] Add a 10% sodium chloride solution by mass to the swim bladder after the degreasing treatment, ...
Embodiment 3
[0045] A method for preparing a biocornea by using swim bladders. The swim bladders of fresh carp are degreased, desugared and freeze-thawed after removing impurities, and then decellularized liquid is added to obtain the acellular matrix of the swim bladders. The acellular matrix is used to contain light-transmitting The artificial biological cornea was obtained by reconstituting the alcohol-water mixture of sex regulator.
[0046] Concrete preparation method is:
[0047] Fresh carp swim bladders are cleaned of impurities and then added with degreasing liquid, which is a mixed solution of A liquid and B liquid, said A liquid is a mixed liquid of dichloromethane, trichloroethylene and tetrachloroethylene, and said B liquid is methanol, ethanol and glycerol, the volume ratio of liquid A to liquid B is 3:1, the speed of the control shaker is 80 r / min, and the degreasing treatment is 1h;
[0048] Add 3% sodium chloride solution in the swim bladder after degreasing treatment, c...
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