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Biological cornea prepared from swimming bladder as well as preparation method and application of biological cornea

A swim bladder and cornea technology, applied in the field of medical materials, can solve the problems of destroying the original structure and composition of the matrix, affecting the transparency and mechanical strength, and the transparency of inflammatory reactions, so as to improve light transmission, improve or restore vision, and biocompatibility sex good effect

Active Publication Date: 2022-03-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the carrier scaffold materials used for the construction of tissue engineering artificial cornea mainly include allogeneic cornea (human), xenogeneic acellular cornea (pig), natural biological materials, and artificial synthetic materials, but there are certain shortcomings and deficiencies: xenogeneic acellular cornea Corneal stroma, such as porcine cornea, will destroy the original structure and composition of the stroma during the decellularization process, affecting its transparency and mechanical strength. It may also cause inflammatory reactions and decrease in transparency after transplantation; natural biological materials such as collagen, chitosan, fibrin The degradation rate of scaffolds made of these materials is too fast and the mechanical strength is not good; artificial synthetic materials sometimes face the problem of poor biocompatibility, and their degradation products may have certain toxic effects on organisms.

Method used

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  • Biological cornea prepared from swimming bladder as well as preparation method and application of biological cornea
  • Biological cornea prepared from swimming bladder as well as preparation method and application of biological cornea
  • Biological cornea prepared from swimming bladder as well as preparation method and application of biological cornea

Examples

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Effect test

Embodiment 1

[0029] A method for preparing a biocornea by using swim bladders. The swim bladders of fresh Spanish mackerel are degreased, desugared, and freeze-thawed after removing impurities, and then added with decellularization solution to obtain the acellular matrix of the swim bladders. The artificial biological cornea was obtained by reconstituting the alcohol-water mixture of the photomodulator.

[0030] Concrete preparation method is:

[0031] Add a mixed solution of dichloromethane and ethanol after removing impurities from the fresh mackerel swim bladder, the volume ratio of dichloromethane and ethanol is 0.1:1, the speed of the shaker is controlled at 50 r / min, and the degreasing treatment is 0.5h;

[0032] Add 1% sodium chloride solution in the swim bladder after degreasing treatment, control the shaker speed to 50r / min, and desugar for 0.5h;

[0033] After the desugar treatment, the swim bladder was frozen and thawed, and the sodium lauryl sulfate solution with a mass percen...

Embodiment 2

[0037]A method for preparing a biocornea by using swim bladders. The swim bladders of fresh silver carp are degreased, desugared and freeze-thawed after removing impurities, and then decellularized liquid is added to obtain the acellular matrix of the swim bladders. The artificial biological cornea was obtained by reconstituting the alcohol-water mixture of the photomodulator.

[0038] Concrete preparation method is:

[0039] Add degreasing liquid after the swim bladder of fresh silver carp removes impurities, and described degreasing liquid is the mixed solution of A liquid and B liquid, and described A liquid is the mixed liquid of carbon tetrachloride and trichloroethylene, and described B liquid is methanol and ethanol The mixed solution, the volume ratio of A liquid and B liquid is 10:1, the speed of the control shaker is 150 r / min, and the degreasing treatment is 2h;

[0040] Add a 10% sodium chloride solution by mass to the swim bladder after the degreasing treatment, ...

Embodiment 3

[0045] A method for preparing a biocornea by using swim bladders. The swim bladders of fresh carp are degreased, desugared and freeze-thawed after removing impurities, and then decellularized liquid is added to obtain the acellular matrix of the swim bladders. The acellular matrix is ​​used to contain light-transmitting The artificial biological cornea was obtained by reconstituting the alcohol-water mixture of sex regulator.

[0046] Concrete preparation method is:

[0047] Fresh carp swim bladders are cleaned of impurities and then added with degreasing liquid, which is a mixed solution of A liquid and B liquid, said A liquid is a mixed liquid of dichloromethane, trichloroethylene and tetrachloroethylene, and said B liquid is methanol, ethanol and glycerol, the volume ratio of liquid A to liquid B is 3:1, the speed of the control shaker is 80 r / min, and the degreasing treatment is 1h;

[0048] Add 3% sodium chloride solution in the swim bladder after degreasing treatment, c...

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Abstract

The invention discloses a biological cornea prepared from swimming bladders as well as a preparation method and application of the biological cornea. Fresh swimming bladders are subjected to impurity removal, degreasing, desugaring and freeze thawing treatment, and then a decellularized solution is added to obtain a decellularized matrix of the swimming bladders; and carrying out collagenous fiber reconstruction treatment on the acellular matrix by using an alcohol-water mixed solution containing a light transmission regulator to obtain the artificial biological cornea. The swim bladder is used as a material for preparing the biological cornea, the obtained biological cornea is very close to a natural cornea in component, has excellent optical properties and excellent mechanical properties, shows excellent biocompatibility and is free of cytotoxicity through in-vitro cell culture, and cornea cells can be normally adhered and grow on the surface of the material, so that the biological cornea can be used for preparing the biological cornea. And a compact cell layer is formed and can be used as a medical material in corneal transplantation and corneal lamella repair. The carrier stent is wide in raw material source, low in production cost, convenient to store and use and capable of meeting the requirements of a large number of carrier stents of tissue engineering corneas.

Description

technical field [0001] The invention relates to the field of medical materials in medicine and bioengineering, in particular to a biological cornea prepared from swim bladders, a preparation method and application thereof. Background technique [0002] Corneal disease is one of the world's major eye diseases and an important cause of blindness. The incidence rate in many countries is increasing year by year, and it has become the second leading cause of blindness in some developing countries in recent years. The lesions of the corneal stroma are often caused by the destruction of the arrangement of the stromal collagen lamina. It takes several months or even years for the corneal stroma to heal, and the healing wound will also produce scars that affect the clarity of the cornea. Therefore, stroma lesions usually affect vision. Corneal diseases of the stromal layer include central corneal thinning and conical keratoconus protruding forward; most of them show autosomal domin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3604A61L27/3641A61L27/3687A61L27/3691A61L27/50A61L2430/16A61L2430/40A61L2300/412Y02A40/81
Inventor 蒋波王宁馨罗振宇尹越朱凌璇刘彬
Owner SICHUAN UNIV
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