Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Regeneration method of sample tube for multi-parameter protein stability analyzer

A technology of stability analyzer and sample tube, which is used in cleaning methods, chemical instruments and methods, cleaning methods and utensils using liquids, etc., can solve the problems of high cost, reuse, and inability to wash, and achieves excellent regeneration effect. The effect of avoiding corrosion

Active Publication Date: 2022-03-15
TSINGHUA UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The regeneration of quartz products currently adopts ordinary acid bubbles, spraying, drying and other processes, but for the sample tube of this application, because the inner and outer diameters of the capillary are very small, conventional methods cannot wash it to a level that can be reused
Moreover, the cost of this sample tube is very high. The price of a single sample tube is more than 45 US dollars. If it is used for one time, it is very expensive for the user and will greatly increase the cost of the laboratory.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Regeneration method of sample tube for multi-parameter protein stability analyzer
  • Regeneration method of sample tube for multi-parameter protein stability analyzer
  • Regeneration method of sample tube for multi-parameter protein stability analyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for regenerating sample tubes using sodium hydroxide solutions of different pH values, comprising the following steps:

[0036] Step 1. After the protein stability experiment is over, rinse the protein solutions in the three sample tubes with ultrapure water, and place them in three sodium hydroxide solution regeneration agents with pH values ​​of 11.0, 10.0, and 9.0 respectively. 30 minutes.

[0037] Step 2. Place the sample tube soaked in the regenerant in an ultrasonic instrument, and use ultrapure water to sonicate for 60 seconds.

[0038] Step 3. Rinse the ultrasonically treated sample tube with ultrapure water to rinse the remaining regenerant.

[0039] Step 4. Finally, use compressed air to blow dry, and the first regeneration experiment of the sample tube is over.

[0040] Step 5, compare the Tm values ​​of different water-soluble protein solutions before and after sample tube regeneration, the coefficient of variation CV% calculated by formula 1, and...

Embodiment 2

[0054] A method for regenerating sample tubes using hydrochloric acid solutions of different pH values, comprising the following steps:

[0055] Step 1. After the protein stability test is over, rinse the protein solutions in the three sample tubes with ultrapure water, and place them in three hydrochloric acid solution regeneration agents with pH values ​​of 3.0, 2.0, and 1.5 for 30 minutes. .

[0056] Step 2. Place the sample tube soaked in the regenerant in an ultrasonic instrument, and use ultrapure water to sonicate for 60 seconds.

[0057] Step 3. Rinse the ultrasonically treated sample tube with ultra-pure water to rinse the residual regenerant.

[0058] Step 4. Finally, use compressed air to blow dry, and the first regeneration experiment of the sample tube is over.

[0059] Step 5. Compare the Tm values ​​of different water-soluble protein solutions before and after sample tube regeneration, the coefficient of variation CV% calculated by formula 1, and the coinciden...

Embodiment 3

[0073] The data result that step 9 obtains in embodiment 1 and 2 is as Figure 5 as shown, Figure 5 The sodium hydroxide solution regeneration sample tube with pH value 11.0 shown in 5 times obtains the variation coefficient CV% of four kinds of proteins to be 0.31%, 0.78%, 1.87%, 0.31% respectively; The hydrochloric acid solution regeneration sample tube 5 with pH value 3.0 The coefficients of variation of the four proteins obtained after the second time were 0.82%, 0.77%, 1.88%, and 0.57%, respectively. The Tm values ​​of the four protein solutions obtained by regenerating the sample tubes five times are very close, and the coefficients of variation are all controlled within 10%, and the regeneration effect of the regenerant used is remarkable. Figure 6 The fluorescence signal curve and the static light scattering signal curve of the IgG protein solution were obtained after the sample tube was regenerated five times with the sodium hydroxide solution with a pH value of 11...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a regeneration method of a sample tube for a multi-parameter protein stability analyzer. After a sample tube is subjected to a regeneration experiment, the regeneration effect is excellent through data analysis, the coefficient of variation is smaller than 10%, and the optimal coefficient of variation can be within 1%. Meanwhile, the number of regeneration times can reach 5, and the user cost in the final technical service process can be reduced to 15% of the original cost. If an alkaline solution with the pH value of 9.0-11.0 or an acidic solution with the pH value of 1.5-3.0 is selected as the regenerant, corrosion of strong acid and strong alkali to the sample tube can be effectively avoided, and meanwhile the situation that weak acid and weak alkali cannot achieve the regeneration effect is avoided. If the soaking time of the sample tube is 20-60 minutes or the ultrapure water ultrasonic time is 30-120 seconds, the regeneration effect can be guaranteed, and meanwhile, the experiment time cannot be wasted too long.

Description

technical field [0001] The invention relates to regeneration of sample tubes, in particular to a regeneration method for sample tubes of multi-parameter protein stability analyzers. Background technique [0002] Proteins are the main executors of the physiological functions of living organisms, and the natural conformation of proteins is the basis for their biological functions. Therefore, the stability research of protein structures is widely used in basic life science research, new drug development, drug molecule design and screening, and biological drug preparations. Development, biomolecular structure optimization and other fields. Existing protein / antibody / vaccine stability detection technologies and methods mainly include: incubator method combined with size exclusion chromatography (SEC), PCR technology (dye method), DSC technology and DSF technology (dye method). [0003] Multi-parameter protein stability analyzer (model: Uncle; specifications: width 54cm×depth 50cm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B08B3/12B08B13/00B08B9/023B08B9/032F26B21/00
CPCB08B3/12B08B13/00B08B9/023B08B9/0321F26B21/001
Inventor 范仕龙王乐乐
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com