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Application of biomarker in preparation of gestational diabetes diagnostic reagent

A biomarker and gestational diabetes technology, applied in the field of medical diagnosis, can solve the problems of unclear pathogenesis of GDM, not conducive to improving maternal and child outcomes, and poor patient compliance

Active Publication Date: 2022-03-11
杭州凯莱谱精准医疗检测技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional basis for GDM diagnosis is to take 75g of sugar orally at 24-28 weeks of pregnancy, such as glucose and tolerance test. It is carried out at this time, the timing is late, and the patient's compliance is not good. The adverse effects of high blood sugar and related metabolic disorders on mother and child have already occur, which is not conducive to improving maternal and child outcomes
Since the pathogenesis of GDM is still unclear, markers and early detection methods that accurately reflect the GDM phenotype are still lacking

Method used

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  • Application of biomarker in preparation of gestational diabetes diagnostic reagent
  • Application of biomarker in preparation of gestational diabetes diagnostic reagent
  • Application of biomarker in preparation of gestational diabetes diagnostic reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] Example 1: Collection of Serum Samples

[0119]Serum samples from normal pregnant women and gestational diabetes were collected from 30 individuals in the second trimester (20-28 weeks) of pregnancy and confirmed by the gold standard test.

Embodiment 2

[0120] Example 2: Extraction of serum metabolites

[0121] According to the ratio of 1:4, methanol precipitant containing various isotope internal standards was added to the serum sample, shaken for 3 minutes, and then centrifuged at 4000 × g for 10 minutes at 20 °C. Take 4 parts of 100 μL supernatant from each sample to 4 sample plates, blow dry with nitrogen, and add various complex solutions containing isotopic internal standards for subsequent UPLC-MS / MS detection.

Embodiment 3

[0122] Example 3: Detection of extracted serum metabolites and data preprocessing

[0123] (1) Liquid chromatography / mass spectrometry conditions

[0124] The four UPLC-MS / MS methods were performed using ACQUITY 2D UPLC (Ultra Performance Liquid Chromatography; Waters, Milford, MA, USA) coupled with Q Exactive (QE) high-resolution mass spectrometry (Thermo Fisher Scientific, SanJose, USA). The mass spectrometer parameters are: scanning resolution 35000, scanning range 70-1000 m / z.

[0125] The specific parameters of the four UPLC-MS / MS methods are as follows:

[0126] Method 1: QE was detected by positive ion electrospray ionization (ESI) mode, and the liquid phase was separated by a C18 chromatographic column (UPLCBEH C18, 2.1x100 mm, 1.7 μm; Waters), and the mobile phase was 0.05% PFPA (pentafluoropropane anhydride) and 0.1% FA (formic acid) in water (A) and methanol (B);

[0127] Method 2: QE was detected by negative ion electrospray ionization (ESI) mode, and the liquid...

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Abstract

The invention relates to the field of medical diagnosis, in particular to biomarkers which are screened by metabonomics and are used for identifying and diagnosing gestational diabetes mellitus, and a model which is used for diagnosing whether a gestational individual is diabetes mellitus or not is established according to the biomarkers. The invention provides a biomarker and a diagnostic model for differential diagnosis of gestational diabetes mellitus, which can be applied to early diagnosis or prediction of gestational diabetes mellitus and have important significance in prevention or treatment of gestational diabetes mellitus.

Description

technical field [0001] The present invention relates to the field of medical diagnosis, in particular, it uses metabolomics to screen diabetes biomarkers and is used for the diagnosis of diabetes, especially the diagnosis of gestational diabetes and a system for diagnosing whether a pregnant individual is diabetic. Background technique [0002] Metabolomics is a discipline that conducts qualitative and quantitative analysis of small molecular metabolites in the body with a relative molecular weight less than 1000. Metabolomics analysis can reflect the physiological and pathological conditions of the body, and can also distinguish the differences between different individuals. With the development of mass spectrometry, liquid chromatography coupled with mass spectrometry (LC-MS) has become the most important research tool in metabolomics research. At present, metabolomics has been widely used in the field of clinical diagnosis, mainly to discover metabolic markers related to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N30/86G16B5/00G16H50/50
CPCG01N33/6893G01N30/8675G16B5/00G16H50/50G01N2800/042G01N2800/368
Inventor 张超孔子青康洲阳陈荣昌刘华芬
Owner 杭州凯莱谱精准医疗检测技术有限公司
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