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Microbial protection composition as well as preparation method and application thereof

A composition and microorganism technology, applied in the preservation of microorganisms and other directions, can solve the problems of complex components, allergic reactions, and limited application of the composite protective agent, and achieve the effects of broadening the applicability, good protection, and accurate detection methods.

Pending Publication Date: 2022-03-11
SHENZHEN XBIOME BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composite protective agent has complex ingredients and contains animal-derived skim milk powder, so its application is relatively limited. At the same time, the ingredients in dairy products may cause allergic reactions in some people

Method used

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  • Microbial protection composition as well as preparation method and application thereof
  • Microbial protection composition as well as preparation method and application thereof
  • Microbial protection composition as well as preparation method and application thereof

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preparation example Construction

[0062] The present invention also provides the preparation method of above-mentioned microbial protection composition, comprises the following steps:

[0063] S1. Dissolve the polysaccharide in water, stir or high shear, and sterilize for later use;

[0064] S2, dissolving the acidic amino acid and the basic amino acid in water, stirring, and sterilizing for later use, to obtain a composition of the acidic amino acid and the basic amino acid;

[0065] S3. Mix the polysaccharide solution obtained in step S1 with the composition of acidic amino acid and basic amino acid obtained in step S2 to obtain the microorganism protection composition.

[0066] Preferably, the method used for the sterilization in the above step S1 is to sterilize at 110-122°C for 15-30 minutes.

[0067] Preferably, the method of sterilization in the above step S2 is to sterilize at 110-122°C for 15-30 minutes or use a filter membrane with a diameter less than / equal to 0.45 micron to filter the bacteria.

Embodiment 1-32

[0068] Embodiment 1-32 microbial protection composition and preparation method thereof

[0069] Each composition raw material composition (mass ratio) is shown in the following table:

[0070]

[0071]

[0072]

[0073] Preparation:

[0074] S1. Dissolve the polysaccharide in water, stir for 30 minutes, sterilize at 110°C for 15 minutes, and set aside;

[0075] S2. Stir the amino acid composition until it is completely dissolved, filter and sterilize it with a filter membrane with a diameter equal to 0.45 microns, and set aside;

[0076] S3. Mix the polysaccharide solution obtained in step S1 and the amino acid composition obtained in step S2 to obtain the microorganism protection composition.

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Abstract

The invention provides a microbial protection composition as well as a preparation method and application thereof, and relates to the pharmaceutical field of microorganisms and new medicines. The microbial protection composition comprises a composition of polysaccharide, acidic amino acid and basic amino acid. The preparation method comprises the following steps: S1, dissolving polysaccharide, stirring or performing high shearing, and sterilizing; s2, dissolving acidic amino acid and alkaline amino acid in water, stirring and sterilizing; s3, mixing the polysaccharide solution and the amino acid solution to obtain the microbial protection composition. The microorganism protection composition provided by the invention is simple in component and wide in application range, and has a good protection effect on various microorganisms. The preparation method of the microbial protection composition is simple in process and low in cost, and has a good industrial application prospect.

Description

technical field [0001] The invention belongs to the field of microorganisms and new medicines and pharmaceuticals, and in particular relates to a microorganism protection composition and its preparation method and application. Background technique [0002] Intestinal flora is the "forgotten organ" of the human body and is closely related to human health. The imbalance of intestinal flora has been confirmed to be related to many diseases. Restoring the imbalance of intestinal flora through fecal microbiota transplantation (FMT) has been applied in clinical practice. Fecal microbiota transplantation is to transplant the functional flora in the feces of healthy people into the gastrointestinal tract of patients to rebuild new intestinal flora and realize the treatment of intestinal and extraintestinal diseases. A number of clinical medical guidelines and consensus have recommended fecal microbiota transplantation for the treatment of recurrent or refractory Clostridium diffici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 黄松谭验
Owner SHENZHEN XBIOME BIOTECH CO LTD
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