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Marker for colorectal cancer or precancerous lesion and application thereof

A technology for precancerous lesions and colorectal cancer, applied in the field of tumor detection, can solve problems such as not being suitable for colorectal cancer screening and detection, and achieve good application prospects, high sensitivity and excellent specificity.

Active Publication Date: 2022-03-08
WUHAN AIMISEN LIFE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the public's acceptance of colorectal cancer blood testing is higher than that of colorectal cancer stool testing. Currently, only Septine 9 has been commercialized as a marker for blood testing. A Meta analysis (Diagnostic accuracy of methylated SEPT9 for blood -based colorectal cancer detection: a systematic review and meta-analysis. Clin TranslGastroenterol.2017; 8(1):e216.) The results showed that the sensitivity of Septine 9 for stage I colorectal cancer was 45%, and the sensitivity for polyps was 15%, making it less suitable for screening detection of colorectal cancer, so it is necessary to further search for methylation markers and their combinations suitable for blood

Method used

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  • Marker for colorectal cancer or precancerous lesion and application thereof
  • Marker for colorectal cancer or precancerous lesion and application thereof
  • Marker for colorectal cancer or precancerous lesion and application thereof

Examples

Experimental program
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Embodiment 1

[0042] This embodiment provides a nucleic acid combination for detecting colorectal cancer or precancerous lesions, which includes the first nucleic acid combination.

[0043] The first nucleic acid combination comprises primer pair 1 (comprising upstream primer and downstream primer) and probe 1, and the base sequence of primer pair 1 is as shown in SEQ ID NO.1-2; The base sequence of probe 1 is as shown in SEQ ID NO. .5 shown.

[0044] NTMT1 upstream primer: TAGTAGGGATCGTAGTAGTTTTCGT, SEQ ID NO.1;

[0045] NTMT1 downstream primer: ACGCCAAACCCGAAATAAAC, SEQ ID NO.2;

[0046] NTMT1 probe: TCGTCGGGTCGCGGTGAGTAA; SEQ ID NO.5.

Embodiment 2

[0048] This embodiment provides a nucleic acid combination for detecting colorectal cancer or precancerous lesions, which includes a second nucleic acid combination.

[0049] The second nucleic acid combination includes primer pair 2 and probe 2, the base sequence of primer pair 2 is shown in SEQ ID NO.3-4, and the base sequence of probe 2 is shown in SEQ ID NO.6.

[0050] MAP3K14-AS1 upstream primer: GTGAGAGTATATGGGTGTGTATCGC, SEQ ID NO.3;

[0051] MAP3K14-AS1 downstream primer: CTAAAAAACAAATCGACCCGC, SEQ ID NO.4;

[0052] MAP3K14-AS1 probe: AGGGGTTGAGGTTGGGAGTCGTAGT; SEQ ID NO.6.

Embodiment 3

[0054] This embodiment provides a nucleic acid combination for detection of colorectal cancer or precancerous lesions, which includes the first nucleic acid combination in Example 1 and the second nucleic acid combination in Example 2.

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Abstract

The invention discloses a marker for colorectal cancer or precancerous lesions and application thereof, and relates to the technical field of tumor detection. The marker of colorectal cancer or precancerous lesion is a CpG site of the NTMT1 gene. Through the methylation level of the CpG site of the NTMT1 gene, early screening or auxiliary diagnosis of colorectal cancer or precancerous lesions can be realized. The NTMT1 gene has very high sensitivity and specificity on a colorectal cancer sample or a precancerous lesion sample in an excrement sample. Through verification, the detection sensitivity and the specificity of the methylation of the NTMT1 gene are far higher than those of SDC2, TFPI2, MAP3K14-AS1 and Septine 9 genes, and the sensitivity of the NTMT1 gene to I / II stage colorectal cancer and progression stage adenoma in a blood sample and the specificity of the NTMT1 gene to other intestinal diseases are also better.

Description

technical field [0001] The invention relates to the technical field of tumor detection, in particular to a marker for colorectal cancer or precancerous lesions and its application. Background technique [0002] Colorectal cancer is one of the most common malignant tumors in the world, mainly including colon cancer and rectal cancer. According to the national cancer statistics released by the National Cancer Center in 2019, the number of new cases of colorectal cancer in my country in 2015 was 376,300, and the number of deaths due to colorectal cancer was 191,000, ranking third in the incidence and mortality of malignant tumors. fifth place. The prognosis of colorectal cancer is related to the severity of the disease at diagnosis. [0003] Sporadic colorectal cancer mainly progresses in the order and pattern of normal-adenomas-carcinoma, and early detection can significantly reduce mortality. Colonoscopy is the gold standard for the diagnosis of colorectal cancer, but its a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 周谛晗粱嘉琳孙帆张玮
Owner WUHAN AIMISEN LIFE TECH CO LTD
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