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Triple PCR detection kit for duck circovirus, duck adenovirus and duck tembusu virus

A technology of duck Tembusu virus and duck circovirus, applied in the field of molecular biology, can solve the unseen problems of triple PCR detection and diagnosis, and achieve the effects of saving detection time, fast and simple operation, and easy operation

Pending Publication Date: 2022-03-01
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no research on the detection and diagnosis of DuCV, DAdV and DTMUV using triple PCR technology

Method used

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  • Triple PCR detection kit for duck circovirus, duck adenovirus and duck tembusu virus
  • Triple PCR detection kit for duck circovirus, duck adenovirus and duck tembusu virus
  • Triple PCR detection kit for duck circovirus, duck adenovirus and duck tembusu virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This example provides a method for establishing a triple PCR reaction system for duck circovirus, duck adenovirus and duck Tembusu virus.

[0045] (1) Design the primer set.

[0046] (a) by comparing the duck circovirus sequences published on the reference GenBank, the duck circovirus Cap gene is selected, and the specific primer pair (the first primer pair) for the Cap gene fragment is designed and screened, the primers For the nucleotide sequences shown in SEQ ID No.1 and SEQ ID No.2;

[0047] (b) compare the duck adenovirus sequences published on the reference GenBank, select the duck adenovirus Hexon gene, design and screen the specific primer pair (second primer pair) for the Hexon gene fragment, and the primer pair has The nucleotide sequences shown in SEQ ID No.3 and SEQ ID No.4;

[0048] (c) By comparing the sequences of Duck Tembusu virus published on the reference GenBank, the E gene of Duck Tembusu virus was selected, and the specific primer pair (the third...

Embodiment 2-9

[0065] This example provides a method for establishing a triple PCR reaction system for duck circovirus, duck adenovirus and duck Tembusu virus.

[0066] The difference from Example 1 is that in this example, the addition amounts of the first primer pair, the second primer pair and the third primer pair are shown in Table 1:

[0067] Table 1

[0068]

[0069]

Embodiment 10-14

[0071] This example provides a method for establishing a triple PCR reaction system for duck circovirus, duck adenovirus and duck Tembusu virus.

[0072] The difference from Example 1 is that in this example, during the PCR reaction, the annealing temperatures were 52.5°C (Example 10), 54.1°C (Example 11), 55.9°C (Example 12), and 57.4°C (Example 13), 58.4°C (Example 14).

[0073] performance test 1

[0074] (1) The PCR reactions obtained in Examples 1-9 were verified by 1.2% agarose gel electrophoresis, and the results were taken and saved.

[0075] figure 1 For the agarose gel electrophoresis figure of the PCR product that embodiment 1-9 provides, figure 1 M in is DNA MarkerDL2000, and 1-9 correspond to Examples 1-9 respectively.

[0076] Depend on figure 1 It can be seen that in the present disclosure, the addition amounts of the first primer pair, the second primer pair and the third primer pair are 1 μL, 1 μL and 2 μL respectively, and the effect is the best when the...

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PUM

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Abstract

The invention relates to a triple PCR (Polymerase Chain Reaction) detection primer group for duck circovirus, duck adenovirus and duck tembusu virus. The triple PCR detection primer group comprises a first primer pair, a second primer pair and a third primer pair, wherein the first primer pair has a nucleotide sequence pair as shown in SEQ ID NO.1 and SEQ ID NO.2; the second primer pair has a nucleotide sequence pair as shown in SEQ ID NO.3 and SEQ ID NO.4; the third primer pair has a nucleotide sequence pair as shown in SEQ ID NO. 5 and SEQ ID NO. 6. The invention further provides a triple PCR detection kit for the duck circovirus, the duck adenovirus and the duck tembusu virus, the detection kit disclosed by the invention can be used for simultaneously detecting the mixed infection or single infection condition of the duck circovirus, the duck adenovirus and the duck tembusu virus, the workload is reduced, and the detection time is greatly shortened; and the purpose of rapidly detecting pathogens is achieved.

Description

technical field [0001] The disclosure relates to the technical field of molecular biology, in particular to a triple PCR detection kit for duck circular ring, duck adenovirus and duck Tembusu virus, in particular to a triple PCR detection kit for duck circular ring, duck adenovirus and duck Tembusu virus A PCR detection primer set and a detection kit obtained therefrom. Background technique [0002] Duck circovirus (Duck circovirus, DuCV) is an important cause of chronic wasting and immunosuppressive infectious diseases in ducks. The virus can infect ducks of different breeds and growth stages. In recent years, the infection of DuCV in ducks and its mixed infection cases have shown an obvious upward trend. Simple infection with DuCV can lead to disordered feathers, growth retardation, and weight loss in infected ducks. Because the symptoms of the disease are not obvious, they are easy to be ignored. However, DuCV infection mainly violates the immune system of the host, cau...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/701C12Q1/686
Inventor 刘成司振书李玉保郭尚敬朱明霞裴兰英路建彪
Owner LIAOCHENG UNIV
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