Brevibacillus laterosporus and application thereof

A technology of Brevibacillus and Laterosporus, applied in Brevibacillus Laterosporus and its application field, to achieve the effects of broad antibacterial spectrum, good safety performance and improved feeding safety

Active Publication Date: 2022-03-01
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports on Bacillus in vitro antibacterial and safety evaluation, but there are few reports on the intervention and repair of intestinal inflammation in animals

Method used

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  • Brevibacillus laterosporus and application thereof
  • Brevibacillus laterosporus and application thereof
  • Brevibacillus laterosporus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 , strain screening

[0061] 1. Materials and methods

[0062] 1.1 Samples: Tissue materials such as roots, stems, and leaves of the medicinal plant honeysuckle.

[0063] 1.2 Pathogenic bacteria

[0064] The strains tested are listed in Table 1.

[0065] Table 1 Pathogen number and source

[0066]

[0067] 1.3 Isolation medium

[0068] Improved MRS medium: peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.05 %, Tween-80 0.1%, pH 6.5; used to isolate lactic acid bacteria.

[0069] LB medium: 0.5% glucose, 1.0% peptone, 0.5% beef extract, 0.5% sodium chloride, adjust pH to 6.5; used to isolate bacteria such as Bacillus.

[0070] PDA medium: 20% potato (boiled juice), 20g glucose, 15g agar, natural pH; used to isolate fungi.

[0071] 1.4 Fermentation medium

[0072] Lactic acid bacteria fermentation medium is MRS liq...

Embodiment 2

[0097] Example 2 , strain identification and safety test

[0098] 1. Materials and methods

[0099] 1.1 Bacterial species: the bacterial strain BLCC1-0716 screened in Example 1 of the present invention.

[0100] Experimental animals: 40 healthy female Kunming mice (body weight 20±2g) were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0101] 1.2 Routine biochemical identification of bacteria: Unless otherwise specified, the general morphology, physiological and biochemical tests were carried out according to "Bergey's Bacterial Identification Manual" (9th edition) and "Microbiology Experiment Manual".

[0102] 1.3 16S rDNA identification of strains

[0103] 1.3.1 Amplification and sequence analysis of 16S rDNA

[0104]The target strain was inoculated in fresh fermentation medium and cultured for 20 hours. The DNA of the bacteria was extracted with a kit from Tiangen Company, and its 16SrDNA sequence was amplified. The primers used are bacterial 16S...

Embodiment 3

[0127] Example 3 , Determination of strain tolerance

[0128] 1. Materials and methods

[0129] 1.1 Strains: Brevibacillus dissporogenes BLCC1-0716 screened out in Example 1 of the present invention;

[0130] 1.2 culture medium is the same as the LB medium of embodiment 1

[0131] 1.3 Method

[0132] 1.3.1 Determination of bile salt tolerance

[0133] Take 1 mL of Brevibacillus lateralosporus BLCC1-0716 seed solution (about 80% spore rate) cultured on a shaker at 37°C for 24 hours to 50 mL of liquid LB medium, and add different volumes of bile salt mother solution to make the final bile salt concentration 0.1% respectively , 0.3% and 0.5%, cultured at 37°C for 4h, and samples were taken at 0h and 4h for counting viable bacteria.

[0134] 1.3.2 Determination of acid resistance

[0135] Take 0.3 mL of Brevibacillus lateralosporus BLCC1-0716 seed solution (spore rate about 80%) cultured on a shaker at 37 °C to 15 mL of liquid LB medium with different pH values ​​(2.5, 3.5,...

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Abstract

The invention provides brevibacillus laterosporus, which is named as brevibacillus laterosporus BLCC1-0716, the brevibacillus laterosporus BLCC1-0716 is preserved in the China Center for Type Culture Collection on November 11, 2021, and the preservation number of the brevibacillus laterosporus BLCC1-0716 is CCTCC (China Center for Type Culture Collection) NO: M 20211397. The bacterial strain is obtained by screening and separating from the tissue of a drug source plant honeysuckle flower, is wide in antibacterial spectrum, good in antibacterial performance, good in tolerance and good in safety, and has good functions of resisting inflammation, protecting intestinal tracts and preventing and treating enteritis.

Description

technical field [0001] The application relates to the field of biotechnology, in particular to a strain of Brevibacillus sporogenes and its application. Background technique [0002] The information disclosed in the Background of the Invention is intended to enhance the understanding of the general background of the invention, and the disclosure should not necessarily be construed as an acknowledgment or any form of suggestion that the information has become the prior art that is already known to those skilled in the art. [0003] With the high-density and intensive development of the breeding industry, the breeding environment of livestock and poultry is getting worse and worse, seriously affecting the production level and health of animals. The abuse of antibiotics leads to the emergence of drug-resistant bacteria, drug residues and environmental pollution, which poses a safety hazard to human health. Therefore, the research and application of new green additives to repla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61P1/00A61P1/04A61P1/12A23K10/18A23L33/135C12R1/01
CPCC12N1/20A61K35/742A61P1/00A61P1/04A61P1/12A23L33/135A23K10/18A23V2002/00A23V2200/32Y02A50/30
Inventor 谷巍徐海燕辛国芹汪祥燕单宝龙孙尹双翟延庆王红陈雷张永奎李金敏曹斌郝木强兰江华郑军红崔海英
Owner 山东宝来利来生物工程股份有限公司
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