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Anthurium andraeanum callus induction method

A technology of callus and anthurium, applied in the field of induction of anthurium callus, can solve the problems of long induction period and low induction rate

Pending Publication Date: 2022-03-01
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the technical problems of low induction rate and long induction period of Anthurium callus in the induction process, the present invention provides a method for inducing Anthurium callus

Method used

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  • Anthurium andraeanum callus induction method
  • Anthurium andraeanum callus induction method
  • Anthurium andraeanum callus induction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Screening of the best parts

[0020] At noon on a sunny day, the young leaves of Anthurium anthurium within 2 weeks (kept the petiole) were collected and grown well. They were quickly brought back to the laboratory, rinsed with running water for 20 minutes, soaked in 75% alcohol for 30s-1min, and then disinfected with 0.1% mercury chloride8 -10min, rinse with sterile water 3-5 times, and set aside. The specific disinfection treatment time depends on the tenderness of the material, and the criterion is that no disinfection damage occurs on the surface of the explant.

[0021] The treated leaves and petioles were respectively inoculated on the medium of: 1 / 2MS+6-BA 0.5mg / L+TDZ 1.0mg / L+agar powder 4.5g / L+sucrose 30g / L+coconut juice 5%. Extraction method of explants: petioles are cut in turn from the position near the base of the leaf into 1cm-long sections, which are numbered B 1 , B 2 , B 3 . The leaves are cut into small squares of 1cm*1cm from the position near the...

Embodiment 2

[0024] Plant Growth Additive Combination Screening

[0025] At noon on a sunny day, the young leaves of Anthurium anthurium within 2 weeks (kept the petiole) were collected and grown well. They were quickly brought back to the laboratory, rinsed with running water for 20 minutes, soaked in 75% alcohol for 30s-1min, and then disinfected with 0.1% mercury chloride8 -10min, rinse with sterile water 3-5 times, and set aside. The specific disinfection treatment time depends on the tenderness of the material, and the criterion is that no disinfection damage occurs on the surface of the explant.

[0026] On the ultra-clean workbench, cut the pretreated petiole (near the blade end) into small sections with a length of about 1 cm, and set aside.

[0027] Inoculate the pretreated petiole (near the leaf end) into the culture medium containing different plant growth regulating substances respectively, the specific dosage of the plant growth regulating substance is shown in Table 1, 50 ex...

Embodiment 3

[0036] Coconut juice concentration screening

[0037] At noon on a sunny day, the young leaves of Anthurium anthurium within 2 weeks (kept the petiole) were collected and grown well. They were quickly brought back to the laboratory, rinsed with running water for 20 minutes, soaked in 75% alcohol for 30s-1min, and then disinfected with 0.1% mercury chloride8 -10min, rinse with sterile water 3-5 times, and set aside. The specific disinfection treatment time depends on the tenderness of the material, and the criterion is that no disinfection damage occurs on the surface of the explant.

[0038] On the ultra-clean workbench, cut the pretreated petiole (near the blade end) into small sections with a length of about 1 cm, and set aside.

[0039] The pretreated explants were respectively inoculated in the medium that added different concentrations of coconut milk, and the specific consumption of coconut milk was as follows: 2%, 5% and 10%, taking the medium without adding any coconu...

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Abstract

The invention discloses an anthurium andraeanum callus induction method, and belongs to the technical field of plant tissue in-vitro culture. According to the method disclosed by the invention, young leaf stalks (near leaf base ends) of the anthurium andraeanum, which are extracted within 2 weeks and have not expanded leaves, are taken as explants, and 6-benzylaminopurine, phenyl thiadiazole urea and coconut juice are added into a 1 / 2 MS culture medium, so that the success rate of tissue culture of the anthurium andraeanum and the quantity and quality of callus induction are improved. According to the method, the technical problems of low induction rate and long induction period in the anthurium andraeanum callus induction process can be solved technically, and the anthurium andraeanum callus induction success rate can reach 99% or above on the premise of getting rid of operation reasons.

Description

technical field [0001] The invention belongs to the technical field of plant tissue in vitro culture, and in particular relates to an anthurium callus induction method. Background technique [0002] Plant tissue culture refers to the cultivation of plant tissues or organs using artificial medium under sterile conditions to regenerate complete plants. Plant tissues or organs used in tissue culture are called explants. The theoretical basis of plant tissue culture is the theory of plant cell totipotency. The complete plant tissue culture process includes: primary culture, proliferation culture and rooting culture. The primary culture is a crucial link in the tissue culture process. According to the classification of the induction purpose, the primary culture can be divided into: dormant bud germination induction and callus induction. [0003] In anthurium tissue culture, the best method for primary culture is to induce callus; in the process of callus induction, the part of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/002
Inventor 卢绪娟庄乾兴
Owner NANTONG UNIVERSITY
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