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Application of TOP1 as cervical cancer marker and/or therapeutic target

A cervical cancer and drug technology, applied in the field of biomedicine, can solve the problems of reduced drug sensitivity and efficacy, and the high price of bevacizumab

Pending Publication Date: 2022-02-25
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the application of molecular targeted drugs in the treatment of cervical cancer, the main monoclonal antibody drug bevacizumab targeting vascular endothelial growth factor can improve the quality of life of patients with recurrent and metastatic cervical cancer, but the price of bevacizumab Expensive, long-term use can cause drug sensitivity and reduced efficacy

Method used

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  • Application of TOP1 as cervical cancer marker and/or therapeutic target
  • Application of TOP1 as cervical cancer marker and/or therapeutic target
  • Application of TOP1 as cervical cancer marker and/or therapeutic target

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1 Immunohistochemistry (IHC) verification

[0107] In the embodiment of the present invention, 90 cases of clinical samples were collected from the Pathology Department of the Second Affiliated Hospital of Chongqing Medical University, including benign cervical lesions, cervical epithelial dysplasia and cervical intraepithelial neoplasia, and cervical cancer tissues. Using immunohistochemical method, the expression level of TOP1 in the above tissue samples was detected, the experimental results are as follows figure 1 As shown, the results of pathological staining showed that the expression of TOP1 increased with the development of the disease process.

Embodiment 2

[0108] Example 2 Bioinformatics software analysis of clinical patient prognosis

[0109] In the embodiment of the present invention, use the GEPIA (http: / / gepia.cancer-pku.cn / ) database to analyze the prognosis of clinical patients, input the gene name: TOP1, select Overall Survival or Disease Free Survival, and customize the color: High group -Red, Low group-Blue, click add to add the cancer type you are interested in: CESC, click plot, and you will get the survival analysis results of cervical cancer patients in the TOP1 high expression and low expression groups.

[0110] The result is as figure 2 and image 3 As shown, compared with TCGA-derived normal tissues, the expression of TOP1 was significantly increased in cervical cancer (eg figure 2 ). Survival analysis showed that cervical patients with high expression of TOP1 had shorter survival time and poorer prognosis than those with low expression of TOP1 (such as image 3 ).

Embodiment 3

[0111] Example 3 Western Blot

[0112] Select the cells in the logarithmic growth phase to extract proteins, and use the SDS-PAGE kit for Western blot analysis. The analysis results are as follows: Figure 4 As shown, compared with HPV-negative cervical cancer cell C33A, the expression of TOP1 was significantly increased in HPV-positive cervical cancer cells Siha and Hela.

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Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to application of TOP1 serving as a prognostic marker and a potential target of HPV positive cervical cancer in preparation of cervical cancer diagnosis tools and treatment preparations, and the TOP1 comprises an amino acid sequence as shown in sequence NO: 1. According to the technical scheme for application of the TOP1 target spot, provided by the invention, in the embodiment of the invention, it has been proved that the proliferation and migration levels of tumors are inhibited after the TOP1 is silenced, the progress of cervical cancer is hindered, and a good technical effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of TOP1 as a cervical cancer marker and / or therapeutic target. Background technique [0002] In 2018, the International Agency for Research on Cancer announced the main infectious pathogens associated with cancer worldwide, such as Helicobacter pylori (HP), high-risk human papillomavirus (HPV), hepatitis B virus (HBV) and hepatitis C virus ( HCV), which collectively cause more than 90% of infection-related cancers worldwide. Among them, HPV infection is mainly distributed in sub-Saharan Africa and Asia. The virus is closely related to many benign lesions of genitals and skin, as well as malignant tumors such as cervical cancer, head and neck cancer, and anal cancer. As the fourth most common cancer in women in the world, cervical cancer is also the leading cause of cancer death among women in developing countries. According to the World Health Orga...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/573G01N33/574C12N9/90C12N15/61A61K45/00A61P35/00
CPCC12Q1/6886G01N33/573G01N33/57411G01N33/57484C12N9/90A61K45/00A61P35/00C12Y599/01002C12Q2600/158C12Q2600/118G01N2333/99
Inventor 罗莹喻琳洪世垣
Owner CHONGQING MEDICAL UNIVERSITY
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