Trichoderma atroviride C61

A technology of Trichoderma dark green and microbial strains, applied in the field of microorganisms, can solve the problems of plant growth inhibition, affecting plant membrane system, photosynthesis and in vivo enzyme activity, etc.

Active Publication Date: 2022-02-18
BIOTECH CENT OF SHANDONG ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, phenolic acids and their derivatives also affect the membrane ...

Method used

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  • Trichoderma atroviride C61
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  • Trichoderma atroviride C61

Examples

Experimental program
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Embodiment 1

[0028] The invention provides a strain of Trichoderma dark green ( Trichoderma atroviride ) C61, was deposited on December 08, 2020 at the General Microbiology Center of China Committee for the Collection of Microbial Cultures, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO. 21035. The specific screening method of this Trichoderma dark green C61 is as follows:

[0029] Configure PDB medium (take 200g of potatoes, boil in distilled water for 30min, filter through gauze and cool to 1L to obtain potato water; take 100mL of potato water, 900mL of distilled water, p-hydroxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid, 100 mg each of ferulic acid, benzoic acid, vanillin, salicylic acid and cinnamic acid, mixed evenly, prepared into phenolic acid liquid medium, put the liquid medium into test tubes, autoclaved), scraped the activated Test the spores of the strain, add sterile water and shake, pass through ...

Embodiment 2

[0031] 1. Degradation of mixed phenolic acids in PDB medium by Trichoderma atroviride C61

[0032] 1.1 Preparation of standard solution and preparation of standard curve: use 50% methanol as solvent to accurately prepare 9 kinds of phenolic acid mixed standards (p-hydroxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid, ferulic acid, benzoic acid, vanillin, salicylic acid, cinnamic acid), in which the concentrations of p-hydroxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid, ferulic acid, benzoic acid, vanillin, and salicylic acid are 200, 100, 50 , 25, 12.5, 6.25, 3.125mg / L, the concentration of cinnamic acid is 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78125mg / L respectively, after passing through a 0.22μm filter membrane, it is measured by high performance liquid chromatography. Get the linear regression equation of each phenolic acid concentration X and peak area Y;

[0033] 1.2 Determination of the content of phenolic acid and its derivatives in the me...

Embodiment 3

[0051] Trichoderma dark green ( Trichoderma atroviride ) C61 alleviates the autotoxic effect of phenolic acids and their derivatives on plants

[0052] Pot test: The test soil is taken from the experimental field of the Institute of Ecology, Shandong Academy of Sciences, sieved to remove impurities, and the water content is measured. The dry soil amount is 1000g / pot and put into a flowerpot (19cm×16cm), and 100mL of p-hydroxybenzoic acid and vanillic acid are added. , syringic acid, p-coumaric acid, ferulic acid, benzoic acid, vanillin, salicylic acid, cinnamic acid 9 kinds of mixed solutions of phenolic acids, so that the concentration of each phenolic acid in the soil is 30mg / g dry soil; After C61 grows on the PDA plate for 5 days, scrape the spores, add sterile water and pass through 4 layers of sterile lens paper, the resulting filtrate is the spore suspension, and after the spore suspension is counted by a hemocytometer, adjust the concentration of the spore suspension to...

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Abstract

The invention relates to trichoderma atroviride C61, and belonging to the technical field of microorganisms. The trichoderma atroviride C61 is preserved in the China General Microbiological Culture Collection Center (CGMCC), No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China on December 8, 2020, with an accession number being CGMCC No. 21035. The invention also provides application of the trichoderma atroviride C61 in degradation of phenolic acids and derivatives thereof. The trichoderma atroviride C61 can degrade nine kinds of mixed phenolic acids with a concentration of 100 mg/L, wherein ferulic acid, benzoic acid, salicylic acid and cinnamic acid are completely degraded within 2 days; p-hydroxybenzoic acid, syringic acid and p-coumaric acid are completely degraded within 3 days; and vanillic acid and vanillic aldehyde are completely degraded within 6 days. Meanwhile, the trichoderma atroviride C61 can also effectively relieve autotoxicity effect of the phenolic acid and derivatives thereof on plants and repaire soil with continuous cropping obstacles.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Trichoderma dark green C61. Background technique [0002] Autotoxic substances are one of the main factors leading to continuous cropping obstacles, and phenolic acids and their derivatives are a type with strong activity, which are released to In the soil environment, and accumulate in the soil, it will significantly change the physical and chemical properties of the soil. The structure and diversity of microbial communities increase the proportion of soil-borne pathogenic microorganisms and increase the incidence of plant diseases. At the same time, phenolic acids and their derivatives also affect the membrane system, photosynthesis and enzyme activity of plants, thereby inhibiting plant growth. The accumulation characteristics of phenolic acids and their derivatives in continuous cropping soil have been confirmed in various plants such as rice, soybean, peanut, cucu...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P21/00C09K17/14C12R1/885C09K101/00
CPCA01N63/38C09K17/14C09K2101/00
Inventor 李红梅魏艳丽杨凯李纪顺扈进冬
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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