Nano-selenium particle system, preparation method and application thereof, regulatory immune cells and pretreatment method of regulatory immune cells

A technology of immune cells and nano selenium, which is applied in the fields of blood/immune system cells, biochemical equipment and methods, nanotechnology, etc. The effect of improving efficacy

Pending Publication Date: 2022-02-15
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no safe and effective way to further enhance the effector function of immune cells before reinfusion of immune cells

Method used

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  • Nano-selenium particle system, preparation method and application thereof, regulatory immune cells and pretreatment method of regulatory immune cells
  • Nano-selenium particle system, preparation method and application thereof, regulatory immune cells and pretreatment method of regulatory immune cells
  • Nano-selenium particle system, preparation method and application thereof, regulatory immune cells and pretreatment method of regulatory immune cells

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Experimental program
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preparation example Construction

[0061] Correspondingly, the present application also provides a preparation method of the above nano-selenium particle system, for example, may include the following steps: loading caffeine on the elemental selenium nanoparticles.

[0062] Specifically, the selenium source solution containing compounded selenium, caffeine solution, reducing agent solution and dispersion stabilizer solution are mixed and reacted, and the chemical reaction mechanism is as follows: figure 1 shown.

[0063] In the above mixing process, on the one hand, the selenium source containing combined selenium undergoes a redox reaction with the reducing agent, thereby being reduced to simple selenium; on the other hand, caffeine is loaded on the surface and inside of the simple selenium through mixing, and the dispersion stabilizer It is used to disperse the reactants uniformly in the system and maintain the stability of the reaction system.

[0064] In some preferred embodiments, the selenium source solu...

Embodiment 1

[0093]The present embodiment provides a kind of preparation method of nano-selenium particle system, specifically as follows:

[0094] (1) Preparation of each raw material:

[0095] A, prepare selenium source solution: accurately weigh 8.647 grams of sodium selenite (Na 2 SeO 3 ), was dissolved in 1 liter of ultrapure water to prepare a sodium selenite solution with a concentration of 50 millimolar (mM).

[0096] B. Preparation of reducing agent solution (taking vitamin C solution as an example): Accurately weigh 88.065 grams of vitamin C (ascorbic acid), dissolve it in 1 liter of ultrapure water, and prepare a vitamin C solution with a concentration of 500 millimolar (mM).

[0097] C, prepare caffeine solution: accurately weigh 1.942 grams of caffeine (chemical formula: C 8 h 10 N 4 o 2 , molecular weight: 194.194), dissolved in 1 liter of ultrapure water to prepare a caffeine solution with a concentration of 10 millimolar (mM).

[0098] D, preparation of dispersion st...

Embodiment 2

[0103] The concentration of the selenium source in the selenium source solution containing combined selenium is 0.5mol / L, the concentration of the reducing agent in the reducing agent solution is 1mol / L, and the concentration of caffeine in the caffeine solution is 100mmol / L L.

[0104] The volume ratio of the selenium source solution to the reducing agent solution is 1:3. The volume of the caffeine solution is 0.5 times the total volume of the selenium source solution and the reducing agent solution. The mass ratio of the total mass of the caffeine solution and the selenium source solution to the dispersion stabilizer solution is 100:0.1.

[0105] The reaction was carried out at 20°C for 48h.

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Abstract

The invention discloses a nano-selenium particle system, a preparation method and an application thereof, regulatory immune cells and a pretreatment method thereof, and belongs to the technical field of biological materials. The nano-selenium particle system comprises a nano-selenium-caffeine compound, and the nano-selenium-caffeine compound comprises elemental selenium nanoparticles and caffeine loaded on the elemental selenium nanoparticles. The preparation method is simple, easy to operate, high in yield, high in caffeine loading rate and good in particle system stability. The nano-selenium particle system can obviously enhance the immune effect function of cytotoxic immune cells (including NK cells, gamma delta T cells, CD8+T cells and the like) by reducing the generation of immunosuppressive metabolites in the cells, so that the final purpose of enhancing the anti-tumor effect function is achieved. The nano-selenium particle system can be used for pretreatment of immune cells, achieves the effect of improving the efficacy of the immune cells, and has very wide market prospects and industrialization value.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a nano-selenium particle system, a preparation method and application thereof, regulatory immune cells and a pretreatment method thereof. Background technique [0002] Selenium is a beneficial and essential trace element to the human body. The lack of selenium can lead to a series of diseases, such as cardiomyopathy, myocardial function decline, Keshan disease, Kaschin-Beck disease, susceptibility to infection, aggravation of liver disease, and decreased sperm motility in men, etc. . However, combined selenium (inorganic selenium), such as sodium selenite (Na 2 SeO 3 ), has a negative effect on the function of cells, including affecting cell activity, etc., and at the same time, it will cause poisoning or even death when the dose is large. [0003] Organic selenium mainly refers to selenium combined with biological macromolecules in the form of coordination bonds ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783B82Y40/00B82Y30/00A61P37/04A61P35/00A61K47/54A61K33/04
CPCC12N5/0646C12N5/0636A61K33/04A61K47/545A61P35/00A61P37/04B82Y40/00B82Y30/00C12N2501/999
Inventor 吴扬哲
Owner JINAN UNIVERSITY
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