Stem cell cryopreservation liquid as well as preparation method and application thereof

Abstract

The invention discloses a stem cell cryopreservation solution as well as a preparation method and application thereof. The cryopreservation liquid is prepared from the following components in percentage by volume: 20 to 40 percent of a basic culture medium, 10 to 20 percent of glycerol, 30 to 60 percent of human serum albumin, 2 to 6 percent of trehalose, 0.5 to 2 percent of glycine or taurine and 6 to 10.5 percent of inositol galactoside or glycoside. According to the cryopreservation liquid, the serum-free cryopreservation liquid is adopted for cryopreservation of the stem cells, the cells are free of damage, the possibility of animal pathogen pollution can be reduced, the inositol galactoside or the glycoside serves as the stabilizer, the cell phenotype can be effectively protected, the activity of the stem cells under the cryopreservation condition can be remarkably improved, and therefore, the physiological function and the biological function of the recovered cells are maintained.

Description

technical field [0001] The invention relates to the technical field of stem cell cryopreservation, in particular to a stem cell cryopreservation solution, its preparation method and application. Background technique [0002] Stem cells are highly proliferative and multi-lineage multifunctional cells with the ability of self-renewal and multi-lineage differentiation. Stem cells are the most primitive cells of living organisms and the origin of all cell lines. They can produce daughter cells with the same phenotype and genotype as themselves, and can also produce specialized cells that make up tissues and organs of the body. At the same time, they can also differentiate for progenitor cells. In adult organs, stem cells can promote tissue repair by continuously dividing and releasing growth factors, and are potential clinical materials for regenerative medicine. [0003] Stem cells with high proliferative activity can obtain a large number of cells after culture, but in the e...

AI Technical Summary

Problems solved by technology

Because low-concentration DMSO has certain toxicity to cells at room temperature, there are certain requirements for the environment and operation of cell cryopreservation, and at the same time, animal serum as an exogenous substance is likely to increase the possibility of animal pathogen contamination. Therefore, a DMSO-free and animal Serum stem cell cryopreservation solution is necessary

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