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Nanobody targeting rage and its application

A nanobody and targeting technology, which is applied in the field of immunology or molecular biology, can solve problems such as the discovery of nanobodies that have not yet been seen, achieve the effects of small molecular weight, low preparation cost, and alleviate pathological changes

Active Publication Date: 2022-06-07
SHENZHEN PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, no nanobodies targeting RAGE have been found

Method used

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  • Nanobody targeting rage and its application
  • Nanobody targeting rage and its application
  • Nanobody targeting rage and its application

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Embodiment Construction

[0031] The preferred embodiments of the present invention will be further described in detail below.

[0032] (1) Expression analysis of RAGE in tumor cells and AD brain tissue

[0033] According to existing reports, the expression of RAGE increases in AD disease state, so this example first compares the expression of RAGE in the brains of normal and AD mice. In addition, RAGE can be expressed in multiple tumor cell lines. In the embodiment, clear renal cancer cells and cell lines are selected to analyze the expression of RAGE in these cells, including the following steps:

[0034] ① Tissue expression analysis of RAGE:

[0035] a), collect the tissue and dehydrate it in 30% sucrose solution at 4°C overnight;

[0036] b), OCT embedded the next day, and stored at -80°C;

[0037] c), place the frozen tissue block in a cryostat to rewarm for 10 minutes before slicing, and the slice thickness is set to 10 μM;

[0038] d), after the sections are slightly dried, they are placed in ...

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Abstract

The present invention provides a nanobody targeting RAGE and applications thereof, wherein the complementarity determining region of the amino acid sequence of the nanobody targeting RAGE is CDR1 as shown in SEQ ID NO:2, as shown in SEQ ID NO:4 CDR2 as shown, CDR3 as shown in SEQ ID NO:6; or CDR1 as shown in SEQ ID NO:9, CDR2 as shown in SEQ ID NO:11, CDR3 as shown in SEQ ID NO:13; or CDR1 as shown in SEQ ID NO:16, CDR2 as shown in SEQ ID NO:18, CDR3 as shown in SEQ ID NO:20. The nanobody of the technical solution of the present invention has a strong combination with RAGE, can block the interaction between RAGE and various receptors, and has a therapeutic effect on AD and cancer.

Description

technical field [0001] The invention belongs to the technical field of immunology or molecular biology, and in particular relates to a nanobody targeting RAGE and its application. Background technique [0002] RAGE (the receptor for advanced glycation end products), the Chinese name is receptor for advanced glycation end products, is a cell surface receptor for advanced glycation end products (AGEs). The accumulation of AGEs in cells and tissues is an important molecular feature of aging. When intracellular proteins survive for too long, AGE protein modification occurs, resulting in the cross-linking of modified proteins and loss of function. AGE modification is an irreversible process. Over time, the accumulation of AGEs increases, the RAGE pathway is activated, and the expression of RAGE increases continuously. In addition to AGEs, important proteins in the process of disease, such as Alzheimer's disease (AD)-related Abeta protein and cancer-related HMGB1 protein, have be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/28C12N15/13C12N1/21C12N15/70G01N33/574G01N33/68A61K39/395A61P25/28A61P35/00A61K49/00A61K49/04B82Y5/00C12R1/19
CPCC07K16/2803C12N15/70G01N33/6896A61P25/28A61P35/00C07K2317/565C07K2317/567C07K2317/569A61K2039/505G01N2333/70503G01N2800/2821G01N33/574A61K39/3955A61K47/6849B82Y5/00C07K2317/92
Inventor 王继刚孙继超王复婧李志杰肖炜
Owner SHENZHEN PEOPLES HOSPITAL
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