A cationic conjugated polymer and ratiometric fluorescent probe based thereon, preparation method and application

A conjugated polymer, fluorescent probe technology, applied in the field of fluorescent probes, can solve problems such as poor selectivity

Active Publication Date: 2022-08-09
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the currently reported CPs system is used for protein detection, and due to the existence of non-specific electrostatic or hydrophobic interactions, it is often easily interfered by other proteins and has poor selectivity.

Method used

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  • A cationic conjugated polymer and ratiometric fluorescent probe based thereon, preparation method and application
  • A cationic conjugated polymer and ratiometric fluorescent probe based thereon, preparation method and application
  • A cationic conjugated polymer and ratiometric fluorescent probe based thereon, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 0.1g of 2,7-dibromo-9,9-bis(6-bromohexyl)fluorene (M1), 0.1g of 2,7-bis(4,4,5,5-tetramethyl-1, 3,2-dioxaboran-2-yl)-9,9-bis(6-bromoethyl)fluorene (M2), 0.1 g of 4,7-bis(5-bromothiophen-2-yl) Benzo[c][1,2,5]thiadiazole (M3) was placed in a three-necked flask, and 25 mL of tetrahydrofuran solution was added to obtain solution 1; 0.5 g of potassium carbonate was mixed with 1 mL of ultrapure water, and added to solution 1 with a syringe , degassed for 30min, added 0.005g of tetrakis (triphenylphosphine) palladium as catalyst, passed nitrogen for 30min, heated and refluxed at 45°C for 6h to carry out the polymerization reaction; after the polymerization reaction, remove the organic solvent with a rotary evaporator, add water and Extraction with dichloromethane, taking the organic phase, drying with anhydrous sodium sulfate, filtering; removing the organic solvent by rotary evaporation to obtain the precursor polymer PF-DBT-Br. After mixing 10 mg of PF-DBT-Br, 1 mL of 1...

Embodiment 2

[0037] Take 0.8g of 2,7-dibromo-9,9-bis(6-bromohexyl)fluorene (M1), 0.4g of 2,7-bis(4,4,5,5-tetramethyl-1, 3,2-dioxaboran-2-yl)-9,9-bis(6-bromoethyl)fluorene (M2), 0.3 g of 4,7-bis(5-bromothiophen-2-yl) Benzo[c][1,2,5]thiadiazole (M3) was placed in a three-necked flask, and 75mL of tetrahydrofuran solution was added to obtain solution 1; 1.38g of potassium carbonate was mixed with 5mL of ultrapure water, and added to solution 1 with a syringe , degassed for 30min, added 0.009g of tetrakis (triphenylphosphine) palladium as a catalyst, passed nitrogen for 30min, heated and refluxed at 60°C for 24h, and carried out the polymerization reaction; after the polymerization reaction, remove the organic solvent with a rotary evaporator, add water and Dichloromethane extraction, take the organic phase, dry with anhydrous sodium sulfate, filter; remove the organic solvent by rotary evaporation to obtain the precursor polymer PF-DBT-Br; 50 mg of PF-DBT-Br, 5 mL of 1-methyl After mixing im...

Embodiment 3

[0040] Take 0.2g of 2,7-dibromo-9,9-bis(6-bromohexyl)fluorene (M1), 0.6g of 2,7-bis(4,4,5,5-tetramethyl-1, 3,2-dioxaboran-2-yl)-9,9-bis(6-bromoethyl)fluorene (M2), 0.9 g of 4,7-bis(5-bromothiophen-2-yl) Benzo[c][1,2,5]thiadiazole (M3) was placed in a three-necked flask, and 35 mL of tetrahydrofuran solution was added to obtain solution 1; 2.5 g of potassium carbonate was mixed with 3.5 mL of ultrapure water, and solution 1 was added with a syringe , degassed for 30min, added 0.017g of tetrakis(triphenylphosphine)palladium as catalyst, passed nitrogen for 30min, heated and refluxed at 100°C for 32h, and carried out the polymerization reaction; after the polymerization reaction, the organic solvent was removed with a rotary evaporator, and water was added. Extract with dichloromethane, take the organic phase, dry with anhydrous sodium sulfate, filter; remove the organic solvent by rotary evaporation to obtain the precursor polymer PF-DBT-Br. After mixing 30 mg of PF-DBT-Br, 6 m...

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Abstract

The invention discloses a ratio-type fluorescent probe for selective quantitative detection of serum albumin, and a preparation method and application thereof. The ratiometric fluorescent probe is a novel self-assembly system of cationic conjugated polymer and sodium dodecyl sulfonate, which has the property of enhancing reverse fluorescence resonance energy transfer within the polymer chain and between molecules. The ratiometric fluorescent probe of the present invention has a specific and significantly enhanced color response to the concentration of serum albumin, which can exclude the interference of other biomolecules in a complex matrix environment, and realize the selective quantitative detection of serum albumin; and the ratiometric fluorescent probe can The needle can greatly reduce the instability of the instrument, the interference of measurement conditions and changes in the concentration of the probe on the detection results, high sensitivity, high accuracy, convenient and fast operation, and is suitable for on-site detection.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, relates to a cationic conjugated polymer and a ratio type fluorescent probe based thereon, a preparation method and application, and specifically designs a novel cationic conjugated polymer and a selective quantitative method based thereon A ratio-type fluorescent probe for detecting serum albumin and its preparation method and application. Background technique [0002] Serum albumin is the most abundant protein in the human body's circulatory system. It has physiological functions such as binding and transporting endogenous and exogenous substances, maintaining blood colloid osmotic pressure, scavenging free radicals, inhibiting platelet aggregation and anticoagulation. important in the process. Since serum albumin is involved in many important biological functions in the human body, the content of serum albumin in human body fluids such as serum and urine is generally regarded as an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G61/12C09K11/06G01N21/64
CPCC08G61/126C09K11/06G01N21/6428C09K2211/1458C09K2211/1466C09K2211/1483C09K2211/1425C08G2261/122C08G2261/143C08G2261/3142C08G2261/3223C08G2261/3246C08G2261/18C08G2261/411C08G2261/522Y02E10/549
Inventor 萨米尔·侯赛因田雪蒙陈曦王悦高瑞霞
Owner XI AN JIAOTONG UNIV
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