Method for strengthening aerobic fermentation of kitchen waste by using oil-removing microbial preparation
A technology of food waste and aerobic fermentation, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large nitrogen loss, composting failure, and low-quality composting products, and achieve improved oil degradation efficiency, promote the production of organic acids, and improve the effect of recycling performance
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Embodiment 1
[0039] One, the preparation of microbial bacterial agent:
[0040] (1) Inoculate Bacillus lysinus, Bacillus hiraeii, and Bacillus licheniformis into shake flasks containing 100 mL of culture medium at a volume ratio of 1%, and inoculate them at 35°C and shake flask rotation speed 160r / min. Cultivate for 24 hours; the medium is: peptone 10g, NaCl 10g, yeast extract 5g, distilled water 1000mL, pH 7.0-7.2.
[0041] (2) Mix the Bacillus lysinus solution, the Bacillus hirai bacteria solution, and the Bacillus licheniformis solution in a volume ratio of 1:1:1, and the cell concentrations of the three strains are 2.05, 3.22, and 1.73 g / L, to obtain the composite microbial bacterial agent.
[0042] 2. Determination of oil degradation rate of single bacteria and compound bacteria agent
[0043] Inoculate the cultured single bacteria or composite bacterial agents in a shaker flask containing 100mL oil medium at a volume ratio of 5%, culture at 35°C and a rotation speed of 160r / min for ...
Embodiment 2
[0045] Embodiment 2: Utilize microorganism to promote the method for high-efficiency aerobic fermentation of kitchen waste
[0046] Proceed as follows:
[0047] (1) Filter and remove impurities from the food waste obtained on the same day, remove paper towels, plastics, bones, etc., and obtain 12 kg of food waste;
[0048] Table 1
[0049]
[0050] (2) Crushing the kitchen waste, the particle size is about 3-5mm, and the moisture is about 70-75%;
[0051] (3) Mix food waste with rice husk and / or straw (moisture content of rice husk or straw is 8%, rice straw, wheat straw, corn straw, rape straw, cotton straw) at a wet weight ratio of 8:2, The total weight is 15 kg, the control C / N is 25-30, the moisture content is about 60%, and it is stirred at room temperature for 30 minutes to make the materials evenly mixed;
[0052] (4) with 2×10 7 CFU / g inoculate the compound microbial agent into the compost mixture, and stir for 30 minutes to make the agent evenly mixed in the co...
Embodiment 3
[0055] Embodiment 3: the method for natural aerobic fermentation of kitchen waste
[0056] Proceed as follows,
[0057] (1) Filter and remove impurities from the food waste obtained on the same day, remove paper towels, plastics, bones, etc., and obtain 12 kg of food waste;
[0058] (2) Crushing the kitchen waste, the particle size is about 3-5mm, and the moisture is about 70-75%;
[0059] (3) Mix kitchen waste and straw with a wet weight ratio of 8:2, with a total weight of 15 kg, control C / N to 25-30, moisture content of about 60%, and stir at room temperature for 30 minutes;
[0060] (4) Put the compost mixture into the compost bucket, control the ventilation rate to 0.2L / (min·L pile body), measure the temperature of the pile body every day, and turn the pile manually on the 3rd, 7th, 14th, 21st, 28th, and 35th days , and take samples to determine relevant indicators, including oil concentration, organic acid concentration, cumulative ammonia discharge, nitrate nitrogen c...
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