Interference RNA for inhibiting AFAP1-AS1 expression and application of interference RNA in increasing breast cancer radiotherapy sensitivity

Abstract

The invention relates to the technical field of biology, and particularly discloses interfering RNA for inhibiting AFAP1-AS1 expression and an application of the interfering RNA in increasing breast cancer radiotherapy sensitivity. The experiments show that long-chain non-coding RNA (lncRNA) AFAP1-AS1 is highly expressed in radiation-resistant breast cancer cells, and the interfering RNA provided by the invention can significantly inhibit expression of lncRNA AFAP1-AS1 in breast cancer cells, can increase radiotherapy sensitivity of breast cancer cells, and has a very positive effect on treatment of breast cancer. The invention also discloses a carrier for carrying the interfering RNA for inhibiting AFAP1-AS1 expression, and the carrier can help consume excessive GSH in radiotherapy resistant tumor cells, break intracellular oxidation-reduction balance and improve the ratio of intracellular reaction active oxygen by adopting a reduction-responsive high-molecular polymer, thereby further improving the radiotherapy sensitivity of the tumor cells. Through combination of radiotherapy and gene therapy, the tumor treatment effect is more obvious.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an interfering RNA that inhibits the expression of AFAP1-AS1 and its application in increasing the radiosensitivity of breast cancer. Background technique [0002] The incidence of cancer is increasing year by year, and it has become one of the most serious diseases threatening human health. The incidence of breast cancer in the world has been on the rise since the late 1970s. [0003] Surgery, chemotherapy, and radiation therapy (radiotherapy for short) are routine cancer treatments. Almost half of all cancer patients receive radiation therapy. Nearly 40% of cured cancer patients benefited from radiotherapy. Most cancer patients are very sensitive to radiotherapy at the beginning of treatment, but patients gradually develop acquired radiotherapy resistance during treatment, which promotes tumor recurrence, invasion and metastasis, and leads to treatment failure. Although increasi...

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Problems solved by technology

However, the synthesis steps of these reduction-responsive nanomaterials are cumbersome, the preparation process is complicated, and the function is single, so they cannot really achieve clinical transformation and application.

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