Viable bacteria type aronia melanocarpa fermented juice and processing method thereof
A processing method and technology of live bacteria, applied in the field of live bacteria fermented berry fruit juice and its processing, can solve the problems of losing the nutritional value of berry fruit, loss of biologically active substances, difficulty in opening up the consumer market, etc., and improve the flavor of fruit juice , maintain ecological balance, regulate the effect of the body's immune response
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Embodiment 1
[0030] S1. Juice pretreatment: Weigh 60 parts of oldberry puree, 5 parts of glucose, and 35 parts of purified water, mix well, and use food-grade NaHCO 3 After adjusting its pH to 4.6, it was homogenized under high pressure twice at 4°C and 15 MPa, and pasteurized at 85°C for 10 minutes, and cooled to 20-30°C to obtain fermented raw juice;
[0031] S2. Strain activation: take out Lactobacillus paracasei from the frozen glycerol tube, inoculate it into MRS liquid medium, cultivate it in a constant temperature incubator at 37°C for 18 hours, take out 1 mL and inoculate it into new MRS liquid medium for secondary expansion , until the bacterial concentration in the culture medium is 10 10 If the cfu / mL is above, stop the culture, centrifuge the bacteria, discard the culture medium, wash the bacteria with sterile normal saline, and resuspend the bacteria with sterile water to obtain a starter;
[0032] S3, inoculation: inoculate the starter into the fermented raw juice according ...
Embodiment 2
[0037] S1. Juice pretreatment: Weigh 50 parts of oldberry puree, 7 parts of glucose, and 43 parts of purified water, mix well, and use food-grade NaHCO 3 After adjusting its pH to 4.8, it was homogenized twice under high pressure at 4°C and 15Mpa, and pasteurized at 85°C for 10 minutes, and cooled to 20-30°C to obtain fermented raw juice;
[0038] S2. Strain activation: After taking out Lactobacillus brevis and Lactobacillus rhamnosus from the frozen glycerol tube, they were inoculated into MRS liquid medium respectively, and after culturing in a constant temperature incubator at 37°C for 18 hours, 1 mL was taken out and inoculated into a new MRS liquid culture medium The base was expanded for the second time until the concentration of the bacterial solution in the medium was 10 10 Cfu / mL or more, stop the culture, centrifuge the bacteria, discard the culture medium, wash the bacteria with sterile normal saline, and resuspend the bacteria with sterile water to obtain a starter...
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