Method for producing triploid oysters through interspecific hybridization of Fujian oysters and Crassostrea sikamea

Abstract

The invention relates to a method for producing triploid oysters through interspecific hybridization of Fujian oysters and Crassostrea sikamea, and belongs to the technical field of aquaculture variety genetic breeding. The method comprises the steps of obtaining of excellent tetraploid Fujian oysters, strong optimization of parents, parent cultivation, artificial insemination and incubation and larva cultivation. According to the method, the female parent of the crassostrea sikamea and the male parent of the tetraploid Fujian oyster are hybridized to obtain the triploid crassostrea sikamea, so that on one hand, the problems of small size and low growth speed are solved on the basis of keeping fine and delicious taste of the crassostrea sikamea, and on the other hand, through the characteristic of high sterility of the triploid, the crassostrea sikamea-Fujian hybrid triploid oysters which can be marketed all year round are obtained.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding of aquaculture varieties, and in particular relates to a method for producing triploid oysters by interspecific hybridization of Fujian oysters and Kumamoto oysters. Background technique [0002] Fujian oyster (Crassostrea angulata), also known as Portuguese oyster, belongs to the family Ostridae and belongs to the genus Crassostrea. It is mainly distributed in the high-temperature and high-salt sea area south of the Yangtze River in my country, mainly in Fujian Province. It has the advantages of fast growth and high yield. , is an important species of oyster culture in my country. [0003] Kumamoto oyster (Crassostrea sikamea), which belongs to the Ostridae family and belongs to the genus Crassostrea, is mainly distributed in the famous seas of Japan, the Korean Peninsula and the central and southern waters of my country, suitable for high temperature, medium and high salinity sea areas, ...

AI Technical Summary

Problems solved by technology

[0005] The tetraploid Fujian oyster has four sets of chromosomes, which can be obtained by fertilizing the ovum of the triploid oyster with normal diploid sperm and inhibiting the release of polar bodies (CN100415085C, CN107494358A, CN109730008A, CN113016673A), and currently produce tetraploid Fujian oyster The induction method has the limitations of instability, low survival rate or success rate, cannot obtain enough tetraploid Fujian oysters, cannot carry out necessary optimization and interspecific hybridization, and has not yet been reported to produce a large number of stable tetraploid Fujian oyster methods

[0006] Different scholars have carried out preliminary research on the interspecific hybridization of diploid Kumamoto oyster and diploid Fujian oyster. At present, there is no report on the crossbreeding of Kumamoto oyster (♀) and tetraploid Fujian oyster (♂) to produce Xiongfu triploid oyster.