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New medical application of propranolol

A technology of tumor drugs and pharmaceutical preparations, applied in the fields of cell biology and medicine, can solve the problem of small side effects of propranolol, and achieve the effect of enhancing the flux of autophagy

Pending Publication Date: 2022-01-14
THE FIRST AFFILIATED HOSPITAL OF XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the clinical application of propranolol has few side effects, and there is no relevant report on the treatment of esophageal squamous cell carcinoma with Prop

Method used

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  • New medical application of propranolol
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  • New medical application of propranolol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Cell viability determination: PBS or PROP (40-100 μm) treatment ECA109, KYSE-450, TE-1 cell 0 h, 24 h, 48 h, 72 h, MTT method to determine cell activity. Experimental results figure 1 As shown, PROP can significantly inhibit the proliferation of human tube squamous cell carcinoma ECA109, KYSE-450, TE-1 cells.

Embodiment 2

[0034] Transwell migration measurement: Set PBS, PROP 60 μm, PROP 80 μm three groups, regular digestion ECA109, KYSE-450, TE-1 cells, serum RPIM 1640 medium retained, adjusted cell density of 5.0 × 10 5 / ml, 600 μL of the lower chamber contain 10% fetal bovine serum medium, put the chamber into the culture plate, add 200 μL to the transport chamber, add 37 ° C, volume fraction 5% CO 2 40 h was cultured in the incubator. Experimental results figure 2 As shown, PROP inhibits human food tube squamous cell carcinoma ECA109, KYSE-450, TE-1 cell migration.

Embodiment 3

[0036] Flow cytometry detection of cell cycle, apoptosis: ECA109, KYSE-450, TE-1 cells PROP 80 μm pretreatment 48 h. Cells collected by centrifugation were washed in pre-cooling PBS and dyed with ANNexin V-FITC and iodide (PI) according to the manufacturer's solution. The cell cycle analysis kit is used to detect the progression of the cell cycle. The cells were pretreated with PROP 80 μm 48 h. The cells collected by centrifugation were washed in pre-cooling PBS and dyed from iodide (PI) according to the manufacturer's solution. Each sample is repeated three times. The data is analyzed by flow cytometry and analyzed through FlowJO-V10 software. Experimental results image 3 As shown, PROP inhibits human food tube squamous cell carcinoma ECA109, KYSE-450, TE-1 cell cycle and promotes apoptosis.

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Abstract

The invention discloses application of propranolol in preparation of drugs for treating tumors, and belongs to the technical field of cytobiology and medicines The invention relates to an effect of propranolol in preparation of esophageal squamous cell carcinoma drugs, propranolol non-selectively blocks beta1 and beta2 adrenergic receptors, and propranolol has the capabilities of inhibiting esophageal squamous cell carcinoma cell proliferation, periodic autophagy, transitional autophagy and nude mouse subcutaneous tumor bearing and promoting apoptosis, which indicates that propranolol can inhibit malignant growth of esophageal squamous cell carcinoma; and propranolol inhibits the metastasis of esophageal squamous carcinoma cells, which indicates that propranolol can inhibit the metastasis of esophageal squamous carcinoma. The application of propranolol in treatment of esophageal squamous cell carcinoma patients provides a new strategy for improving prognosis and even curing the esophageal squamous cell carcinoma.

Description

Technical field [0001] The present invention belongs to the field of cell biology and pharmaceutical technology, and there is a new medical use of propranolol, PrOP. Background technique [0002] my country's esophageal cancer is 17.87 / 100,000, and the 6th place in malignant tumors; mortality is 13.68 / 100,000, and the fourth place of malignant tumors. In 2019, China Multi-center clinical data analysis showed that esophagealsquamous cancer (ESCC) was about 95.5% of the total number of tube cancer. In the treatment and follow-up, ESCC is discovered early transfer, easy to relapse, even if surgery, targeted therapy, biotherapy, etc., the five-year survival rate is still less than 20% in advanced ESCC patients. Therefore, exploring new drugs or new therapies is an urgent need for improving the efficacy and ESCC patient survival. [0003] Propranolol, Prop is a non-selective β1, β2 adrenergic receptor blocker, clinically used to control hypertrophic symptoms, such as hypertension,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/138A61P35/00A61P35/04
CPCA61K31/138A61P35/00A61P35/04
Inventor 赵宝生刘玉珍钱河齐博谷城威霍书华
Owner THE FIRST AFFILIATED HOSPITAL OF XINXIANG MEDICAL UNIV
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