Application of PEFL-1 detection reagent in preparation of lung cancer screening kit

A PEFL-1 and kit technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve problems such as PEFL-1 protein that has not yet been seen, and achieve good application prospects and effective screening results.

Pending Publication Date: 2022-01-11
核工业四一六医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Application of PEFL-1 detection reagent in preparation of lung cancer screening kit
  • Application of PEFL-1 detection reagent in preparation of lung cancer screening kit
  • Application of PEFL-1 detection reagent in preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Relationship Between PEFL-1 in Plasma and Lung Cancer

[0025] 1. Clinical data

[0026] Select 40 cases of lung cancer patients and 40 cases of healthy controls. The basic information is as follows:

[0027]

[0028]

[0029] 2. Detection principle

[0030] Same as the basic enzyme-linked immunosorbent assay (ELISA) detection method.

[0031] PEFL-1 protein PEFL-1 antibody is immobilized on the protein chip, and after incubation with plasma exosome protein, if there is PEFL-1 protein in the exosome protein, PEFL-1 protein will bind to it; wash to remove unbound antibody And other proteins, then use the fluorescently labeled PEFL-1 antibody to bind to the PEFL-1 protein for detection, and read the signal through a fluorescent scanner. The strength of the signal is positively correlated with the affinity and quantity of the antibody.

[0032] 3. Method

[0033] 1. The preparation method of plasma exosomal protein is as follows:

[0034] Plasma sample...

Embodiment 2

[0051] Embodiment 2 The composition of detection kit of the present invention and its application method

[0052] 1. Kit composition

[0053] Detection kit (14 persons):

[0054]

[0055]

[0056] The reagents for preparing plasma exosomal proteins need to be prepared by themselves, which can be commercially available plasma exosomal protein extraction kits.

[0057] 2. How to use the kit

[0058] Same as the third part of Example 1.

[0059] The kit of the present invention can detect the level of PEFL-1 in plasma exosomes, and can screen the risk of lung cancer in the population to be tested: if the level of PEFL-1 is high (relative to patients with benign lung diseases), the risk of lung cancer is high. The risk of lung cancer is high, and if PEFL-1 levels are low (relative to those with benign disease of the lungs), the risk of lung cancer is low. It can be used for auxiliary diagnosis of clinical lung cancer, and provide effective basis for patients to take releva...

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PUM

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Abstract

The invention relates to the field of in-vitro diagnostic reagents, in particular to application of a similar alpha assumed elongation factor 1 Putativeelongation factor 1-alpha-like 3, (PEFL-1) in preparation of a lung cancer screening kit. The invention finds that the PEFL-1 protein level in the plasma exosome of a lung cancer patient is obviously higher than that of a lung benign disease patient for the first time. The reagent for detecting the PEFL-1 protein is used for preparing the lung cancer screening kit, and effective screening of lung cancer can be achieved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of PEFL-1 detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer are very im...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57423G01N33/57488G01N2333/47
Inventor 方凯江志强廖中华龙琴刘舒
Owner 核工业四一六医院
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