Application of 3-methylthiopropionic acid in preparation of nematicidal preparations

A technology of methylthiopropionic acid and methylmalonic acid, which is applied in the field of agricultural microbiology, can solve problems such as non-conformity, low efficiency, and unfriendly chemicals to the environment, and achieve improved insecticidal effects and good sustainability

Active Publication Date: 2022-01-11
HUBEI BIOPESTICIDE ENG RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the efficiency of agricultural control and physical control is low, which does not meet the needs of modern agricultural development; chemical control is the main control method at present, but some chemicals are not friendly to the environment, and the current resistance problem of nematodes has become increasingly serious; biological control It is the use of natural enemies of nematodes or biological natural products with nematicidal activity to control plant nematodes

Method used

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  • Application of 3-methylthiopropionic acid in preparation of nematicidal preparations
  • Application of 3-methylthiopropionic acid in preparation of nematicidal preparations
  • Application of 3-methylthiopropionic acid in preparation of nematicidal preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 3-methionic acid in the preparation of the application of killing southern root-knotted worm preparation, the application process is as follows:

[0022] Pick the nematode egg block from the root of the tomato infection of the southern root nematode, and placed the egg block in the dark incubation of 20 ° C in a petri dish of 15 ml of sterile water, and observe the root knot worm hatch. The nematode is collected by centrifugation and the nematode concentration is calculated.

[0023] Will buy from Aladdin The company's 3-methionic acid was configured to 500 μg / ml of aqueous solution, added to 96-well plates, by adding sterile water to 50, 25, 12.5, 6.25, 3.13. 1.56 μg / ml, 30-40 nematodes per well, the total measurement of 100 μl of surveillance, is a negative control in sterile water, and each group was repeated 3 times. In the 20 ° C incubator, the dark culture 24H post mirror test statistical nematode mortality rate, 1 μl of 5M NaOH solution was added to the 96-well ...

Embodiment 2

[0028] 3-methionic acid in the preparation of the application of the culpron root knot worm preparation, the application process is as follows:

[0029] Picking the nematode egg block from the roots of the tomato infected with javaw, placed the egg blocks at 20 ° C in a petri dish in 15 ml of sterile water, and observed root knobs incubation. The nematode is collected by centrifugation and the nematode concentration is calculated.

[0030] 500 μg / ml of 3-methionacene solution was added to the 96-well plate, and the final concentration of 3-methylthio proppropionic acid was 50, 25, 12.5, 6.25, 3.13, 1.56 μg / ML, each hole is added to 30-40 nematodes, and the total measurement is 100 μL to perform a negative control in sterile water, and each group is repeated 3 times. In the 20 ° C incubator, dark culture 24H post mirror test strain mortality, 3-methionaceous propionic acid to the root of the roots of the claws, shown in Table 2, LC 50 It was 3.56μg / ml.

[0031] Table 2 3 - In...

Embodiment 3

[0035] 3-methionic acid in the preparation of the application of the soybean cyclobioprotic preparation, the application process is as follows:

[0036] From the roots of soybean glycines infected with soybean cyst, the cysts were built into a dust-free disval of 15 ml of a petri dish in 15 ml of sterile water. . The nematode is collected by centrifugation and the nematode concentration is calculated.

[0037] 500 μg / ml of 3-methionacene solution was added to the 96-well plate, and the final concentration of 3-methylthio proppropionic acid was 50, 25, 12.5, 6.25, 3.13, 1.56 μg / ML, each hole is added to 30-40 nematodes, and the total measurement is 100 μL to perform a negative control in sterile water, and each group is repeated 3 times. Placed in a 20 ° C incubator in dark culture 24H post-mirror test straps mortality, 3-methionic acid to soy spore cyst nematodes, LC 50 It was 4.93μg / ml.

[0038] Table 3 - Anti-inctic activity of soybean cyst linear

[0039]

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Abstract

The invention belongs to the technical field of agricultural microbiology and relates to application of 3-methylthiopropionic acid in preparation of nematicidal preparations. It is found that the 3-methylthiopropionic acid has good insecticidal activity on nematodes through determination of the fatality rates of meloidogyne incognita, meloidogyne javanica, soybean cyst nematodes and caenorhabditis elegans; The LC50 values of the 3-methylthiopropionic acid on the meloidogyne incognita, meloidogyne javanica and soybean cyst nematode are 3.34 [mu] g / mL, 3.56 [mu] g / mL and 4.93 [mu] g / mL respectively; and after the 3-methylthiopropionic acid is compounded witha compound preparation is compounded with methylmalonic acid, the minimum LC50 of the compound preparation on the meloidogyne incognita is 2.50 [mu] g / mL. The preparation provides a new resource for research and development of a novel plant nematode killing preparation.

Description

Technical field [0001] The present invention belongs to the technical field of agricultural microbiology, and specific applications involving 3-methylthiopropionic acid in the preparation of killing umbrella preparations. Background technique [0002] More than 200,000 can have been discovered by more than 5,000 pole insects. Among them, the roots of the roots (Meloidogyne SPP.) And cytostats (HETERODERA SPP. And Globodera SPP.) Are the most serious. [0003] Root knots can be parasitized to almost all vasotel plants, including M.incognita, Northern root nematodes (M.haplanda), Javanica and peanuts (M.arenaria) The hazard is the most serious. In recent years, the harm of M.enterolobii is also increasingly aggravated in recent years. The eggs of the rooted nematode will be produced in a lem in a protective lens matrix. The age larvae formed by embryonic development will become an aging larvae with infection, which will migrate in the soil. Looking for a host, once the root is foun...

Claims

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Application Information

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IPC IPC(8): A01N37/36A01N37/04A01P5/00
CPCA01N37/36A01N37/04Y02A50/30
Inventor 陈凌刘晓艳刘南茜王梦思闵勇邱一敏朱镭周荣华田雨曦饶犇廖先清王月莹
Owner HUBEI BIOPESTICIDE ENG RES CENT
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