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Erysipelothrix rhusiopathiae and application thereof

A technology of Erysipelas erythematosus and a preservation number, which is applied in the field of biology, can solve the problems of poor immune effect of Erysipelas erythematosus type 1 and type 2 bacteria, etc., and achieves the effect of significant technological progress.

Pending Publication Date: 2022-01-07
SHANGHAI ANIMAL EPIDEMIC PREVENTION & CONTROL CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of Erysipelothrix rhusiopathiae and its application, said a kind of Erysipelothrix rhusiopathiae and its application will solve the vaccine in the prior art for Erysipelothrix rhusiopathia type 1 and type 2 bacteria immunity Technical Issues That Don't Work Well

Method used

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  • Erysipelothrix rhusiopathiae and application thereof
  • Erysipelothrix rhusiopathiae and application thereof
  • Erysipelothrix rhusiopathiae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 bacterial strain source background

[0019] The strain was isolated in 2015 from the heart of a diseased fattening pig (above 100kg) raised in a pig farm in Fengxian, Shanghai. On the afternoon of the first day, the diseased pigs suffered from lack of energy, red skin, and a fever of 40°C to 41°C. They died suddenly the next morning. The autopsy found enlarged spleen and lymph nodes throughout the body, hemorrhage in the throat and heart, emphysema, and pancreatic edema. , Kidneys are fragile, and other organs have no obvious lesions. The body surface and main necropsy lesions of diseased pigs can be seen in figure 1 A, 1B and 1C.

Embodiment 2

[0020] Example 2 strain isolation and identification

[0021] 2.1 Isolation and culture of bacteria

[0022] 2.1.1 Gently touch the aseptically collected infected pig disease material (including heart, liver, spleen, lung) to the culture medium of Columbia blood plate (added with 5% defibrated sheep blood), and inoculate it with a sterile inoculation loop. at 37°C and 5% CO 2 Environmental cultivation 24h ~ 48h. After culturing for 24 hours (the left picture), pinpoint-sized colonies can be seen at all the inoculation sites of the diseased materials on the medium, showing alpha hemolysis, and the hemolysis circle is narrow grass green; after culturing for 48 hours (the right picture), the colonies become larger to a diameter of 0.5 Around mm, the hemolytic circle becomes larger and the hemolysis is obvious. Such as figure 2 As shown in A.

[0023] 2.1.2 Inoculate suspicious colonies into gelatin medium by puncture, culture at 22°C for 48 hours, and grow like a test tube ...

Embodiment 3

[0046] Example 3 Erysipelothrix rhusiopathiae isolate mouse immune protection test

[0047] 3.1 Experimental animals

[0048] Clean grade BALB / c mice, 18-20g.

[0049] 3.2 Challenge strains

[0050] 3.2.1 Strain: CVCC 43005——type 2 (from China Veterinary Microorganism Culture Collection Management Center), the original 43-5 strain, an inactivated vaccine production strain, and a virulent strain.

[0051] CVCC 4060 (China Veterinary Microbiological Culture Collection and Management Center)—type 1a, former JD-2012 strain, clinical isolate, virulent strain.

[0052] 3.2.2 Determination of minimum lethal dose (MLD)

[0053] 3.2.2.1 Dilute the bacterial solution to 0.5McF (about 1×10 8 CFU / mL).

[0054] 3.2.2.2 Take 10 -3 、10 -4 、10 -5 、10 -6 Four dilutions, 3 mice for each dilution, and 0.1 mL was injected subcutaneously in the neck of each mouse.

[0055] 3.3 Preparation of vaccine

[0056] 3.3.1 Take the freeze-dried strain of SHFX14035B strain (CGMCC No: 23390), place ...

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Abstract

The invention provides erysipelothrix rhusiopathiae. The preservation number of the erysipelothrix rhusiopathiae is CGMCC No: 23390. The invention further provides a vaccine. The vaccine is characterized in by containing inactivated erysipelothrix rhusiopathiae with the preservation number of CGMCC No: 23390. According to the erysipelothrix rhusiopathiae, a plurality of groups of clinically separated strains are screened, and an attacking protection experiment is carried out together with a traditional swine erysipelas vaccine, so that a vaccine candidate strain which has good protection effect on epidemic type 1 and type 2 strains in swine herds in China is found.

Description

technical field [0001] The invention belongs to the field of biology and relates to erysipelothrix, specifically erysipelothrix rhusiopathiae and application thereof. Background technique [0002] Erysipelothrix rhusiopathiae, a member of the genus Erysipelothrix, is a zoonotic pathogen. Susceptible animals are extremely wide, including various livestock, poultry, wild animals, fish, crustaceans, amphibians, etc. Pigs are the main host of Erysipelothrix rhusiopathiae, which causes swine erysipelas, mainly leading to septicemia, arthritis and endocarditis in pigs, causing serious economic losses to the pig industry. Humans can also be infected with Erysipelothrix rhusiopathiae, which mainly manifests as local redness, swelling, pain and other symptoms, which are called "like erysipelas". [0003] The genus Erysipelothrix is ​​currently divided into three species, including Erysipelothrix rhusiopathiae, Erysipelothrix tonsillarum, and Erysipelothrix nopinala. Including 23 s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
CPCA61K39/0241A61P31/04A61K2039/521A61K2039/552
Inventor 徐锋王晓旭王建沈莉萍齐新永赵洪进鞠厚斌杨显超葛菲菲唐聪圣张玉杰白艺兰吴秀娟
Owner SHANGHAI ANIMAL EPIDEMIC PREVENTION & CONTROL CENT
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