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Lactobacillus reuteri capable of improving indole acrylic acid to regulate specific IgE

A technology of Lactobacillus reuteri and microbial strains, applied in the field of microorganisms, to achieve relief, reduce IgE levels, and relieve allergic symptoms

Active Publication Date: 2022-01-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for OVA-induced food allergy, although there are reports on the role of tryptophan metabolism in regulating food allergy, the most mentioned pathway is mainly the kynurenine pathway, and which small molecule metabolite it is specifically It works and how it works, not yet reported

Method used

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  • Lactobacillus reuteri capable of improving indole acrylic acid to regulate specific IgE
  • Lactobacillus reuteri capable of improving indole acrylic acid to regulate specific IgE
  • Lactobacillus reuteri capable of improving indole acrylic acid to regulate specific IgE

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1: the acquisition of Lactobacillus reuteri (Lactobacillus reuteri) CCFM1190

[0080] (1) Screening and identification of Lactobacillus reuteri CCFM1190

[0081] The fermented dairy product from Dali, Yunnan was used as a sample (the sample number is DYNDL11), and the sample was serially diluted with physiological saline, and 100 μL of the diluted solution was used to spread the plate (LBS solid medium, and add 0.5‰ medium volume before pouring the plate) Sterile nystatin), placed in a 37°C constant temperature incubator and cultured upside down for 48 hours, according to the classification of the colony, pick a single colony on the LBS solid medium for plate marking, and then place it in a 37°C constant temperature incubator and culture upside down for 48 hours Pick a single colony and inoculate it into 5mL liquid LBS medium, place it in a constant temperature incubator at 37°C for 16-18h, take 1.5mL of the bacteria and centrifuge at 6000r / min for 3min to re...

Embodiment 2

[0086] Embodiment 2: the cultivation of Lactobacillus reuteri (Lactobacillus reuteri) CCFM1190

[0087] Specific steps are as follows:

[0088] Inoculate Lactobacillus reuteri CCFM1190 and Lactobacillus reuteri L. reuteri 1 into MRS liquid medium respectively, and after culturing at 37°C for 16 hours, draw the bacterial liquid and inoculate it into fresh MRS liquid medium according to the inoculum amount of 4%. In the same condition, cultured for 12 hours, 8000r / min centrifuged the thalline for 15min, washed the thalline with 0.9% normal saline and centrifuged again at 8000r / min for 10min, collected the thalli, resuspended with 30% (m / v) sucrose solution, respectively The resuspension was prepared and frozen at -80°C until use.

[0089] Preparation of bacterial suspension for gavage: when Lactobacillus reuteri is used for gavage to mice, take out the prepared suspension from -80°C, centrifuge at 4°C, 8000r / min for 10min, Discard the supernatant, and resuspend with 0.9% norma...

Embodiment 3

[0090] Example 3: Effect of Lactobacillus reuteri (Lactobacillus reuteri) CCFM1190 on the content of indoleacrylic acid in the intestinal metabolites of mice

[0091] Specific steps are as follows:

[0092] The 4-5 week-old SPF grade BALB / c female mice were divided into 4 groups, namely the normal group, the model group and the experimental group. L. reuteri 1 group of L. reuteri 1;

[0093] 6 animals in each group were raised in the Experimental Animal Center of Jiangnan University, fed with common feed, with a constant temperature of 21-26°C, humidity of 40-70%, noise less than or equal to 60dB, and animal illumination of 15-20LX (all animal experiment procedures were provided by Jiangnan University Animals). Welfare and Ethics Committee review and approval).

[0094] The experimental period was 7 weeks in total, and the first week was the adaptation period. During the adaptation period, feeding: Co60 irradiated experimental mouse growth and reproduction feed (purchased fr...

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Abstract

The invention discloses lactobacillus reuteri capable of improving indole acrylic acid to regulate specific IgE, and belongs to the technical field of microorganisms and the technical field of medicines. The invention provides lactobacillus reuteri CCFM1190. The lactobacillus reuteri CCFM1190 can be used for relieving the pathological characteristics of mice with food allergy, so that the lactobacillus reuteri CCFM1190 which can further relieve food allergy has the specific advantages that the OVA specific IgE level of the serum of the mouse with the food allergy is reduced, the weight loss of the serum of the mouse with the food allergy is relieved, the lesion degree of the serum jejunum tissue of the mouse with the food allergy is relieved, and the allergic symptoms of the mouse with the food allergy are relieved, so that the lactobacillus reuteri CCFM1190 can be used for preparing products for preventing and / or treating food allergy such as food, medicine or health food and the like, andhas a huge application prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus reuteri capable of increasing indoleacrylic acid to regulate specific IgE, and belongs to the technical field of microorganisms and medicine. Background technique [0002] Food allergy refers to the immune response caused by food or food additives, which can lead to digestive system or systemic allergic reaction. With the increase in the incidence of food-borne allergic diseases, food allergy has become one of the important public health problems of global concern, with a high incidence in North America, Europe and Asia. The clinical symptoms of food allergy are complex and diverse, which may include gastrointestinal symptoms, respiratory symptoms or skin symptoms, and the onset time is also different, and may be acute, subacute or chronic, which has a great impact on the health of patients. Since food allergy usually occurs when food is ingested, some of the proteins present in it are considered as a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L19/00A23L11/50A23L2/38A23C9/123A61K35/747A61P1/00A61P37/08C12R1/225
CPCC12N1/20A23L33/135A23L11/50A23L19/00A23C9/1234A23L2/382A61K35/747A61P37/08A61P1/00A23V2002/00A23V2400/173A23V2200/304
Inventor 陆文伟钱黎翟齐啸崔树茂方志锋王刚杨波王鸿超朱金林赵建新陈卫张灏
Owner JIANGNAN UNIV
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