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Preparation method and application of a fluorescent sensor for detecting glyphosate

A fluorescent sensor and glyphosate technology, applied in the field of glyphosate detection, can solve the problems of difficult large-scale routine detection, long detection time, complicated processing, etc., and achieve the effects of easy visual observation, low cost, and high detection sensitivity

Active Publication Date: 2022-05-27
HARBIN UNIV OF SCI & TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the problems of cumbersome product pretreatment, long detection time, high cost and difficulty in large-scale routine detection in the existing method for detecting glyphosate, and provides a method for preparing a fluorescent sensor for detecting glyphosate and application

Method used

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  • Preparation method and application of a fluorescent sensor for detecting glyphosate
  • Preparation method and application of a fluorescent sensor for detecting glyphosate
  • Preparation method and application of a fluorescent sensor for detecting glyphosate

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Effect test

specific Embodiment approach 1

[0051] Embodiment 1: The preparation method of a fluorescence sensor for detecting glyphosate in this embodiment includes the following steps:

[0052] Dissolve the fluorescent probe L in HEPES buffer solvent to prepare 1.0 × 10 -4 ~1.0×10 -6 mol / L solution, add copper nitrate, and incubate for 2 to 5 minutes to obtain a fluorescence sensor;

[0053] The molecular structure of the fluorescent probe L is:

[0054]

specific Embodiment approach 2

[0055] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the volume ratio of DMSO to water in the HEPES buffer solvent is 7:3. Others are the same as the first embodiment.

specific Embodiment approach 3

[0056] Embodiment 3: This embodiment is different from Embodiment 2 in that: the HEPES concentration in the HEPES buffer solution is 10 mM; and the pH is 5-10. Others are the same as in the second embodiment.

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Abstract

A preparation method and application of a fluorescent sensor for detecting glyphosate, relating to the field of glyphosate detection, in particular to a preparation method of a fluorescent sensor and its application in glyphosate detection. It is to solve the problems of cumbersome product pretreatment, long detection time, high cost and difficulty in large-scale routine detection in the existing detection method of glyphosate. Method: The fluorescent probe L was dissolved in HEPES buffer solvent, and copper nitrate was added to incubate to obtain the fluorescent sensor. The fluorescent sensor prepared by the invention has good selectivity to glyphosate, and the sample pretreatment method in the actual detection is simple, free from the interference of other organophosphorus pesticides, with high detection sensitivity, fast speed and low cost, and does not require expensive large-scale Instruments and complex sample pretreatment can realize large-scale and routine detection of glyphosate. The invention is used in the detection field of glyphosate.

Description

technical field [0001] The invention relates to the field of glyphosate detection, in particular to a preparation method of a fluorescence sensor and its application in glyphosate detection. Background technique [0002] Glyphosate is a chronic broad-spectrum biocidal organophosphorus herbicide of systemic conduction, mainly by inhibiting the activity of 5-enolpyruvylshikimate-3-phosphate synthase, preventing shikonin from converting to aromatics in plants. The conversion of amino acids interferes with protein synthesis, resulting in plant death. Compared with other herbicides, glyphosate has the advantages of high efficiency, broad spectrum, non-selectivity, easy decomposition in the environment, and low toxicity to mammals. The most widely used and most productive pesticide variety. [0003] The long-term and extensive use of glyphosate has caused serious environmental pollution and a serious threat to human health. Recent studies have shown that glyphosate can cause a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/16C09K11/06G01N21/64
CPCC07D311/16C09K11/06G01N21/6428G01N21/6447C09K2211/1088
Inventor 由君吴绵园喻艳超刘洋武文菊
Owner HARBIN UNIV OF SCI & TECH
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