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Preparation method and application of fluorescence sensor for detecting glyphosate

A fluorescent sensor and glyphosate technology, applied in the field of glyphosate detection, can solve the problems of difficult large-scale routine detection, time-consuming detection, cumbersome processing, etc., and achieve the effects of easy visual observation, low cost and high detection sensitivity

Active Publication Date: 2021-12-31
HARBIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the problems of cumbersome product pretreatment, long detection time, high cost and difficulty in large-scale routine detection in the existing method for detecting glyphosate, and provides a method for preparing a fluorescent sensor for detecting glyphosate and application

Method used

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  • Preparation method and application of fluorescence sensor for detecting glyphosate
  • Preparation method and application of fluorescence sensor for detecting glyphosate
  • Preparation method and application of fluorescence sensor for detecting glyphosate

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Experimental program
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Effect test

specific Embodiment approach 1

[0051] Embodiment 1: The preparation method of a fluorescence sensor for detecting glyphosate in this embodiment includes the following steps:

[0052] Dissolve the fluorescent probe L in HEPES buffer solvent to prepare 1.0 × 10 -4 ~1.0×10 -6 mol / L solution, add copper nitrate, and incubate for 2 to 5 minutes to obtain a fluorescence sensor;

[0053] The molecular structure of the fluorescent probe L is:

[0054]

specific Embodiment approach 2

[0055] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the volume ratio of DMSO to water in the HEPES buffer solvent is 7:3. Others are the same as the first embodiment.

specific Embodiment approach 3

[0056] Embodiment 3: This embodiment is different from Embodiment 2 in that: the HEPES concentration in the HEPES buffer solution is 10 mM; and the pH is 5-10. Others are the same as in the second embodiment.

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Abstract

The invention discloses a preparation method and application of a fluorescent sensor for detecting glyphosate, relates to the field of glyphosate detection, and especially relates to a preparation method of the fluorescent sensor and application of the fluorescent sensor in glyphosate detection. The invention aims to solve the problems of tedious pretreatment, long detection time, high cost and difficulty in large-scale conventional detection in the existing glyphosate detection method. The method comprises the following steps: dissolving a fluorescent probe L in an HEPES buffer solvent, adding copper nitrate, and incubating to obtain the fluorescent sensor. The prepared fluorescent sensor has good selectivity on glyphosate, a sample pretreatment method in actual detection is simple, interference of other organophosphorus pesticides is avoided, the detection sensitivity is high, the speed is high, the cost is low, expensive large instruments and complex sample pretreatment are not needed, and large-scale and conventional detection of glyphosate can be achieved. The fluorescent sensor is applied to the field of glyphosate detection.

Description

technical field [0001] The invention relates to the field of glyphosate detection, in particular to a preparation method of a fluorescent sensor and its application in glyphosate detection. Background technique [0002] Glyphosate is a systemic conduction type chronic broad-spectrum organic phosphorus herbicide, mainly by inhibiting the activity of 5-enol pyruvyl shikimate-3-phosphate synthase, and hindering the production of shikimic acid in plants. The conversion of amino acids interferes with protein synthesis, leading to plant death. Compared with other herbicides, glyphosate has the advantages of high efficiency, broad spectrum, non-selectivity, easy decomposition in the environment, and low toxicity to mammals. It is widely used in agriculture, forestry, aquatic products and other fields. The most widely used and the largest yield of pesticide varieties. [0003] The long-term and extensive use of glyphosate has caused serious environmental pollution and a serious th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/16C09K11/06G01N21/64
CPCC07D311/16C09K11/06G01N21/6428G01N21/6447C09K2211/1088
Inventor 由君吴绵园喻艳超刘洋武文菊
Owner HARBIN UNIV OF SCI & TECH
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