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Huperzia serrata in-vitro tissue culture and preservation method

A technology of Huperus serpentis and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve the problems of scarcity of resources, no reports of successful tissue culture, and difficulty in sterilizing explants.

Inactive Publication Date: 2021-12-31
董丽辉
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Problems solved by technology

[0003] In order to solve the scarcity of Huperzia serrata resources, tissue culture research on Huperzia serrata at home and abroad has been carried out. However, due to the symbiosis of a large number of fungi in Huperzia serrata, it is difficult to sterilize the explants. So far there has been no successful tissue culture report. To establish a better sterilization method, we use the shoot tip of Hupernia serrata as explants to lay the foundation for further tissue culture

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  • Huperzia serrata in-vitro tissue culture and preservation method
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  • Huperzia serrata in-vitro tissue culture and preservation method

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Embodiment 1

[0024] Such as Figure 1-5 As shown, a kind of Huperzia serrata in vitro tissue culture preservation method of the present embodiment comprises the following steps:

[0025] Step 1: Select the fresh medicinal plant Hupercia serrata collected in different months, soak it in a betaine-type surfactant for 20 minutes, wash it, and rinse it with running water for 6 hours; then sterilize it, rinse it 6 times with sterile water, and put After the disinfection treatment, the shoot tips on the explants were inoculated in different culture media; at 20°C, the light time was 12h / d, and the light intensity was 1250lx, and cultured for 2-3 weeks;

[0026] Step 2: culture on the inoculated medium, then transfer the sterile explants on the surface after 3 weeks of cultivation to the medium added with malachite green and antibiotic solution, continue to cultivate for 4 weeks to kill endophytes, and finally put Sterile explants should be transferred to the original culture medium to continue ...

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Abstract

The invention provides a huperzia serrata in-vitro tissue culture and preservation method. The method comprises the following steps: step 1, selecting a fresh medicinal plant huperzia serrata material collected in different months, soaking the fresh medicinal plant huperzia serrata material in a betaine type surfactant for 20 minutes, washing the fresh medicinal plant huperzia serrata material, and flushing the fresh medicinal plant huperzia serrata material with running water for 6 hours; and then performing sterilizing, flushing the material with sterile water for 6 times, and inoculating the stem tips on disinfected explants into different culture media. The research on rooting of clustered seedlings shows that a basic M2 culture medium is more suitable for rooting compared with a B5 culture medium, the hormone IBA has significant influence on the growth of roots, IBA with higher or lower concentration is not beneficial to rooting of the clustered seedlings, and the rooting state of the clustered seedlings in the MS culture medium added with 10 mu mol / L IBA is optimal.

Description

technical field [0001] The invention relates to the technical field of Huperzia serrata, in particular to a method for culturing and preserving Huperzia serrata isolated tissue. Background technique [0002] Huperus serrata, also known as life-saving king, thousand-story pagoda, and gold does not change, is a fern of Huperzaceae and genus Hupermus, mainly distributed in the Yangtze River Basin, Yunnan, Guizhou, Northeast and Guangdong and Guangxi regions; the whole herb of Huperus serrata is used as medicine, Folks are used to treat carbuncle, furuncle, swollen poison, bruises, etc.; recent studies have shown that the active ingredient of serpentine is huperzine A; it is a powerful acetylcholinesterase inhibitor used to treat myasthenia gravis, which can improve Senile memory loss, compared with other similar products, has less side effects, and is a promising drug for treating senile dementia. Since Huperzine serrata grows very slowly, plant resources are scarce, and the w...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002A01H4/00
Inventor 董丽辉
Owner 董丽辉
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