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Composition for improving, preventing, or treating bone diseases or metabolic diseases, including novel lactobacillus sakei cvl-001 strain and culture medium thereof

A technology of Lactobacillus sarkeri and metabolic diseases, applied in the direction of metabolic diseases, Lactobacillus, bone diseases, etc., can solve the problems of serious obesity and side effects of drugs, achieve the purpose of inhibiting weight gain, improving osteoporosis, preventing Or the effect of treating osteoporosis or obesity

Pending Publication Date: 2021-12-24
IND FOUND OF CHONNAM NAT UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] However, drugs used to treat obesity have serious side effects compared to their effects, and therefore, there is an urgent need to develop substances with a new mechanism of action having excellent anti-obesity effects and low side effects

Method used

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  • Composition for improving, preventing, or treating bone diseases or metabolic diseases, including novel lactobacillus sakei cvl-001 strain and culture medium thereof
  • Composition for improving, preventing, or treating bone diseases or metabolic diseases, including novel lactobacillus sakei cvl-001 strain and culture medium thereof
  • Composition for improving, preventing, or treating bone diseases or metabolic diseases, including novel lactobacillus sakei cvl-001 strain and culture medium thereof

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Embodiment approach

[0102] Hereinafter, the present invention will be further described in detail through examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited to the following examples.

[0103] Throughout this specification, unless otherwise stated, "%" used to express the concentration of a specific substance has the following meanings, that is, solid / solid is (weight / weight) %, solid / liquid is (weight / volume) %, liquid / liquid is (volume / volume)%.

Embodiment 1

[0104] Example 1. Isolation of bacterial strains

[0105] Freshly pickled kimchi was collected from all over the country, and the collected kimchi was stored at a temperature of 6±0.5° C. for 0 to 5 weeks and used as a kimchi sample for isolating lactic acid bacteria. 500 g of the above-mentioned kimchi was ground by a hand blender (handblender, Hanil Co, Korea), and sterilized water was added to the kimchi liquid filtered with sterile gauze in 10 1 to 10 7 Diluted sequentially. Thus, a dilution of 10 7 Fold the kimchi smear medium.

[0106] Apply the diluted kimchi liquid to add 2% calcium carbonate (CaCO 3 ) MRS medium (Difco Co., France), cultured at 30° C. for 48 hours, and isolated the strain that formed the transparent ring. Gram staining (Gram strain kit, BD Co., USA) and catalase test (Biomerieux Co., France) were performed on the isolated above strains to provisionally confirm Gram-positive and catalase-negative colonies for lactic acid bacteria.

[0107]Inocul...

Embodiment 2

[0108] Example 2. Identification of Lactic Acid Bacteria Strains

[0109] The finally screened Lactobacillus sakei CVL-001 was identified by 16S ribosomal RNA base sequence analysis. After the isolated strains were separated by pure water, they were sent to Solgent (Daejeon, South Korea) by refrigerated express in the state of MRS plate (plate) for entrustment, using 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT -3') to perform 16S ribosomal RNA base sequence analysis with a universal primer set.

[0110] A total of 1439 bp base sequence was determined by the 16S ribosomal RNA coding base sequence of the above-mentioned Lactobacillus sarkeri CVL-001 strain, and the results are shown in Table 1.

[0111] Table 1

[0112]

[0113]

[0114] Based on the above-mentioned nucleotide sequence executed, the homology detection of the nucleotide sequence was performed using the registration information (GeneBank database) as the object by the Blast program (...

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Abstract

The present invention relates to a novel Lactobacillus sakei CVL-001 strain and a method for isolating same, wherein the strain is lactic acid bacteria which can be isolated from kimchi, and thus can be effectively used as a probiotic or food additive. Furthermore, the present invention relates to a composition for improving, preventing, or treating bone diseases or metabolic diseases, including Lactobacillus sakei CVL-001 strain (Lactobacillus sakei) or a culture medium thereof, wherein the Lactobacillus sakei strain isolated from kimchi and the culture medium thereof exhibit the effects of suppressing osteoclast differentiation, improving osteoporosis, suppressing adipocyte differentiation, and suppressing weight gain, and thus can be effectively used for the improvement, prevention, or treatment of osteoporosis or obesity related diseases.

Description

technical field [0001] With the support of the Ministry of Science and Technology Information and Communication, this invention was completed through project 2018R1A2B3004143. The research management professional organization of the above project is the Korean Research Foundation. The name of the research project is "Intermediate Researcher Support Project", and the name of the research technology is "Nod2 Mediated research on cell / tissue-specific defense systems", the competent institution is the Industry-University Cooperation Group of Chonnam University, South Korea, and the research period is from March 1, 2018 to February 28, 2022. [0002] This application claims Korean Patent Application No. 10-2019-0022670, No. 10-2019-0022671 filed with the Korean Patent Office on February 26, 2019 and Korean Patent Application No. 10 filed with the Korean Patent Office on February 3, 2020 - Priority of No. 2020-0012763, the entire disclosure of the above-mentioned patent application ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A61K35/747A61P19/08A61P1/02A61P3/04A61P3/06A61P9/12A61P9/00A61P5/48A23L33/135C12R1/225
CPCC12N1/20C12N1/02A61K35/747A61P19/08A61P1/02A61P3/04A61P3/06A61P9/12A61P9/00A61P5/48A23L33/135A23V2002/00A23V2400/179A23V2200/306A23V2200/30A23V2200/328A23V2200/332A23V2200/3262A23V2200/326A23L33/105C12N1/205C12R2001/245A23L33/40A61P19/00A61P19/10C12R2001/225A61K2035/115C12N2500/14
Inventor 朴钟环崔珠熙郑道宪
Owner IND FOUND OF CHONNAM NAT UNIV
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