Tibetan medicine compound for preventing and treating acute altitude stress, and health-care product of Tibetan medicine compound
A technology for altitude sickness and health care products, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, drug combinations, etc., and can solve the problems of non-portable medicines
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Embodiment 1
[0011] Embodiment 1: the preparation of Tibetan medicine compound instant tea of the present invention
[0012] 1. Raw materials 20g coriander root, 150g seabuckthorn, 100g pomegranate seeds.
[0013] 2. Preparation method
[0014] 1) Screen the fresh coriander root, seabuckthorn, and pomegranate seeds of high quality, wash and drain, cut coriander root into thin slices of about 5mm, and place in a vacuum dryer (vacuum degree: 100pa; temperature: -28°C to -30°C) ℃; heating plate temperature; 65°C-70°C; time: 16h) to dry, collect and dry coriander root slices, seabuckthorn, and pomegranate seeds, weigh, pulverize, pass through a 50-mesh sieve, and mix to obtain the crude product of Tibetan medicine combination ( water content is less than 10%), add 10L of water and decoct for 1 hour, naturally cool to normal temperature, filter, discard the filter residue, and obtain 9.64L of crude extract.
[0015] 2) Vacuum freeze-dry the above crude extract (working pressure 40pa, sublim...
Embodiment 2
[0019] Embodiment 2: in vitro antioxidant test
[0020] 1. DPPH free radical scavenging experiment
[0021] 1.1 Experimental method
[0022] Accurately weigh 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), prepare a DPPH solution with a concentration of 0.2mM with absolute ethanol, then pipette 100 μL of DPPH solution into a 96-well plate, each well Add 100 μL of different concentrations of the sample solution to be tested, set up three replicate wells for each concentration, shake well, and incubate at room temperature (20-25°C) in the dark for 30 minutes. The control group uses absolute ethanol instead of the sample to be tested The sample solution was measured, and three duplicate holes were also set up, and the absorbance value A517 of the system at a wavelength of 517nm was measured on a microplate reader, and the experiment was repeated three times in parallel.
[0023] The calculation formula is:
[0024] DPPH free radical scavenging rate (%)=(A control-A sample) / A co...
Embodiment 3
[0050] Example 3: Evaluation of anti-hypoxic activity of HUVEC, H9C2, and SY5Y cells
[0051] 1. Materials and methods
[0052] 1.1 Reagents and instruments
[0053] DMEM culture medium and fetal bovine serum (FBS) were purchased from Gibco; penicillin, streptomycin, and PBS were purchased from Beijing Suo Laibao Technology Co., Ltd.; trypsin was purchased from Gino Biopharmaceutical Technology Co., Ltd.; MTT reagent Box (Wuhan Boster Biological Engineering Co., Ltd.); instruments include BIO-RAD microplate reader (Shandong Brocade Scientific Instrument Co., Ltd., model IMARK).
[0054] 1.2 Cell lines
[0055] Human umbilical vein endothelial cells HUVEC, cardiomyocytes H9C2, and human neuroblastoma cells SY5Y were purchased from ATCC.
[0056] 1.3 Experimental method
[0057] 1.3.1 Cell grouping and establishment of hypoxia model
[0058] The HUVEC, H9C2, and SY5Y cells were divided into 4 groups, namely the control group, the model group, the low-concentration instant t...
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