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Tibetan medicine compound for preventing and treating acute altitude stress, and health-care product of Tibetan medicine compound

A technology for altitude sickness and health care products, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, drug combinations, etc., and can solve the problems of non-portable medicines

Inactive Publication Date: 2021-12-24
西藏藏医药大学 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on the above problems, the present invention provides a Tibetan medicine compound and its health care product for preventing and treating acute altitude sickness. Simple, easy to carry products, to fill the gaps in the products of Tibetan medicine in the prevention and treatment of acute altitude sickness, and solve the problem of non-portable medicines

Method used

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  • Tibetan medicine compound for preventing and treating acute altitude stress, and health-care product of Tibetan medicine compound
  • Tibetan medicine compound for preventing and treating acute altitude stress, and health-care product of Tibetan medicine compound
  • Tibetan medicine compound for preventing and treating acute altitude stress, and health-care product of Tibetan medicine compound

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: the preparation of Tibetan medicine compound instant tea of ​​the present invention

[0012] 1. Raw materials 20g coriander root, 150g seabuckthorn, 100g pomegranate seeds.

[0013] 2. Preparation method

[0014] 1) Screen the fresh coriander root, seabuckthorn, and pomegranate seeds of high quality, wash and drain, cut coriander root into thin slices of about 5mm, and place in a vacuum dryer (vacuum degree: 100pa; temperature: -28°C to -30°C) ℃; heating plate temperature; 65°C-70°C; time: 16h) to dry, collect and dry coriander root slices, seabuckthorn, and pomegranate seeds, weigh, pulverize, pass through a 50-mesh sieve, and mix to obtain the crude product of Tibetan medicine combination ( water content is less than 10%), add 10L of water and decoct for 1 hour, naturally cool to normal temperature, filter, discard the filter residue, and obtain 9.64L of crude extract.

[0015] 2) Vacuum freeze-dry the above crude extract (working pressure 40pa, sublim...

Embodiment 2

[0019] Embodiment 2: in vitro antioxidant test

[0020] 1. DPPH free radical scavenging experiment

[0021] 1.1 Experimental method

[0022] Accurately weigh 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), prepare a DPPH solution with a concentration of 0.2mM with absolute ethanol, then pipette 100 μL of DPPH solution into a 96-well plate, each well Add 100 μL of different concentrations of the sample solution to be tested, set up three replicate wells for each concentration, shake well, and incubate at room temperature (20-25°C) in the dark for 30 minutes. The control group uses absolute ethanol instead of the sample to be tested The sample solution was measured, and three duplicate holes were also set up, and the absorbance value A517 of the system at a wavelength of 517nm was measured on a microplate reader, and the experiment was repeated three times in parallel.

[0023] The calculation formula is:

[0024] DPPH free radical scavenging rate (%)=(A control-A sample) / A co...

Embodiment 3

[0050] Example 3: Evaluation of anti-hypoxic activity of HUVEC, H9C2, and SY5Y cells

[0051] 1. Materials and methods

[0052] 1.1 Reagents and instruments

[0053] DMEM culture medium and fetal bovine serum (FBS) were purchased from Gibco; penicillin, streptomycin, and PBS were purchased from Beijing Suo Laibao Technology Co., Ltd.; trypsin was purchased from Gino Biopharmaceutical Technology Co., Ltd.; MTT reagent Box (Wuhan Boster Biological Engineering Co., Ltd.); instruments include BIO-RAD microplate reader (Shandong Brocade Scientific Instrument Co., Ltd., model IMARK).

[0054] 1.2 Cell lines

[0055] Human umbilical vein endothelial cells HUVEC, cardiomyocytes H9C2, and human neuroblastoma cells SY5Y were purchased from ATCC.

[0056] 1.3 Experimental method

[0057] 1.3.1 Cell grouping and establishment of hypoxia model

[0058] The HUVEC, H9C2, and SY5Y cells were divided into 4 groups, namely the control group, the model group, the low-concentration instant t...

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Abstract

The invention discloses a Tibetan medicine compound for preventing and treating acute altitude stress, and a preparation method of a health-care product of the Tibetan medicine compound. The Tibetan medicine compound is prepared from the following Tibetan medicines in parts by weight: 0.2-0.4 part of Brassica rapa L., 1.2-2.0 parts of fructus hippophae and 1.0-1.5 parts of pomegranate seeds. The prescription is a folk proved prescription derived on the basis of increase and decrease of the prescription of the pomegranate Pu'an powder, the application history is more than thirty years, and in-vivo and in-vitro experiments prove that the Tibetan medicine compound has good anti-oxidation, anti-hypoxia and anti-fatigue effects, few adverse reactions and high safety, has a good prevention effect on the acute altitude stress, and can effectively reduce the incidence rate of the acute altitude stress. Three Tibetan medicines all belong to homology of medicine and food, and the prepared instant tea is sour and sweet in taste, environmentally-friendly, healthy, convenient in carrying and high in consumer compliance, conforms to the principles of three effects, three small and five convenience, and has high use value and a wide development prospect.

Description

technical field [0001] The invention relates to the field of Tibetan medicine and natural medicine, in particular to a Tibetan medicine compound prescription for preventing and treating acute altitude sickness and a preparation method of a health care product thereof. Background technique [0002] Acute altitude sickness is a common disease in high-altitude areas. If it cannot be controlled, it will develop into high-altitude pulmonary edema, high-altitude cerebral edema, and high-altitude pulmonary hypertension. It will seriously endanger the health of tourists and people in Tibetan areas, and affect the economic development and safety work in Tibetan areas. At present, anti-altitude sickness drugs are mainly divided into Chinese herbal medicines, Tibetan medicines, Western medicines, and combined Chinese and Western medicines, etc., to reduce the oxygen consumption rate of the body under hypoxic conditions, increase the oxygen production rate of various tissues and organs, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31A61P43/00A61P39/06A61P9/00
CPCA61K36/31A61K36/185A61P43/00A61P39/06A61P9/00A61K2236/331A61K2236/51A61K2300/00
Inventor 米玛陈静雷海民王鹏龙
Owner 西藏藏医药大学
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