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Method for preparing linolenic acid-rich diglyceride by enzyme method

A technology for the preparation of diglycerides of linolenic acid and enzymatic methods, which is applied in the direction of biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems of high yield of diglycerides, limited reuse times, and high price of lipase, and achieve Reduce production costs, increase the number of reuses, and overcome the high cost of lipase

Pending Publication Date: 2021-12-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic glycerol hydrolysis method has a high yield of diglyceride, and no free fatty acid is produced in the enzymatic hydrolysis process, which has the characteristics of simple subsequent refining process. However, the main obstacle restricting the industrial application of enzymatic glycerolysis in the preparation of diglyceride oil is The price of lipase is high, and the number of reuse is limited, etc.

Method used

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  • Method for preparing linolenic acid-rich diglyceride by enzyme method
  • Method for preparing linolenic acid-rich diglyceride by enzyme method
  • Method for preparing linolenic acid-rich diglyceride by enzyme method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: (reaction temperature optimization)

[0038] (1) The raw material is treated by reducing the peroxide value: the linseed oil with a peroxide value of 6.4mmol / kg is deodorized, and the deodorization condition is 180°C, 2mbar, 80min; kg.

[0039] (2) Synthesis of diglycerides rich in linolenic acid: add glycerol (the molar ratio of glycerol to linseed oil is 1:2) to the linseed oil treated with peroxide value reduction, and then add candida from Antarctica Yeast (Candida antarctica) lipase Lipozyme435, the amount of enzyme added is 10% (w / w, relative to the total mass of reactants), mixed together in a 50mL reactor, quickly heated to a certain temperature, reacted for 8h, and the pressure of the reaction system was controlled Below 80mbar. After the reaction is finished, recover the lipase and remove glycerol to obtain diglyceride rich in linolenic acid, and finally use HPLC to detect the content of diglyceride in the crude product.

[0040] Investigate t...

Embodiment 2

[0043] Embodiment 2: (reaction time optimization)

[0044] (1) The raw material is treated by reducing the peroxide value: the linseed oil with a peroxide value of 6.4mmol / kg is deodorized, and the deodorization condition is 180°C, 2mbar, 80min; kg.

[0045](2) Synthesis of diglycerides rich in linolenic acid: add glycerol (the molar ratio of glycerol to linseed oil is 1:2) to the linseed oil treated with peroxide value reduction, and then add candida from Antarctica Yeast (Candida antarctica) lipase Lipozyme 435, the amount of enzyme added is 10% (w / w, relative to the total mass of reactants), mixed together in a 50mL reactor, rapidly heated to 50°C, reacted for a period of time, the reaction system The pressure is controlled below 80mbar. After the reaction is finished, recover the lipase and remove glycerol to obtain diglyceride rich in linolenic acid, and finally use HPLC to detect the content of diglyceride in the crude product.

[0046] Investigate the influence of re...

Embodiment 3

[0049] Embodiment 3: (reaction system optimization)

[0050] (1) The raw material is treated by reducing the peroxide value: the linseed oil with a peroxide value of 6.4mmol / kg is deodorized, and the deodorization condition is 180°C, 2mbar, 80min; kg.

[0051] (2) Synthesis of diglycerides rich in linolenic acid: add glycerol (the molar ratio of glycerol to linseed oil is 1:2) to the linseed oil treated with peroxide value reduction, and then add candida from Antarctica Yeast (Candida antarctica) lipase Lipozyme 435, the amount of enzyme added is 10% (w / w, relative to the total mass of the reactant), mixed together in a 50mL reactor, and added to the reactor 2 times the total mass of the substrate The organic solvent or no organic solvent was added, then the temperature was rapidly raised to 50° C., and the reaction was carried out for 8 hours. In the solvent-free reaction system, the operating pressure was controlled below 80 mbar. After the reaction, lipase was recovered a...

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Abstract

The invention discloses a method for preparing linolenic acid-rich diglyceride by an enzyme method, and belongs to the technical field of food processing. According to the method disclosed by the invention, the linolenic acid-rich diglyceride is prepared by utilizing an enzymatic glycerolysis method, and the peroxide value in the linolenic acid-rich raw material is reduced by adopting a distillation or adsorption method, so that the aim of retaining the lipase activity to the greatest extent in an enzymatic reaction process is fulfilled, and the repeated use frequency of the lipase in the glycerolysis reaction process is increased, and the cost of preparing diglyceride by the enzyme method is reduced. The method for synthesizing the diglyceride grease through the enzyme method is high in efficiency and low in cost, the problem that lipase is prone to inactivation in the enzymatic reaction process is solved, and the method has great application prospects in grease processing.

Description

technical field [0001] The invention belongs to the technical field of food processing, and relates to a method for preparing structured lipids, in particular to a method for enzymatically preparing diglyceride rich in linolenic acid. Background technique [0002] Oils are usually synthesized by dehydrating esterification of glycerol and fatty acids. Triglyceride (TAG) content is the highest in most oils and fats, accounting for more than 95% of the total amount. Other components include monoglyceride (MAG), diglyceride (DAG), free fatty acids, phospholipids, etc. Diacylglycerol, especially 1,3-diglyceride (1,3-DAG), has a different metabolic pathway than triglyceride, which can inhibit the accumulation of fat in the body and help the body control body weight. At present, the problem of obesity in modern society is becoming more and more serious. As an ideal substitute for triglyceride in edible oil, diglyceride has attracted extensive attention and has a broad market. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N9/22
CPCC12P7/6454C12P7/6472C12N9/22C12Y301/01003
Inventor 王小三陈烨王熠璠赵昕辰
Owner JIANGNAN UNIV
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