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Panoramic rotary endoscopic two-photon microscopic imaging system

A microscopic imaging and two-photon technology, used in microscopes, optics, optical components, etc., can solve the problem of inability to realize two-photon imaging to analyze static and dynamic signals, and achieve excellent results and high efficiency.

Pending Publication Date: 2021-12-17
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the irresistible limitation of single-photon fluorescence excitation at the level of physical principles, nVista is only limited to analyzing the dynamic signals of large-scale neuronal structures (such as cell bodies), and is completely unable to realize the fine anatomical structure ( The ability to resolve static and dynamic signals at scales such as dendritic spines

Method used

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  • Panoramic rotary endoscopic two-photon microscopic imaging system
  • Panoramic rotary endoscopic two-photon microscopic imaging system
  • Panoramic rotary endoscopic two-photon microscopic imaging system

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Refer figure 1 A panoramic rotary endo-photon micromaramography system of the present embodiment, including: near-infrared femtosecond pulse laser 1, host 9, imaging probe assembly 6, and axial scanning device 8, the imaging probe assembly 6 includes a rotary output unit 6.0.2 and Rotary Drive Unit 6.0.1;

[0053] The rotary output unit 6.0.2 is implanted into the brain tissue of the non-human primate 11, which is used to introduce femtosecond pulsed lasers into the brain tissue, and collect fluorescence generated by the brain tissue; the axial scanning device 8 is used for The drive rotation output unit 6.0.2 moves along the Z axis to achieve an axial scan; the rotary drive unit 6.0.1 is used to drive the rotary output unit 6.0.2 to rotate at a high speed around the Z-axis to achieve a 360 degree annular scan;

[0054] The femtosecond pulse laser emitted by the near-infrared femtosecond pulse laser 1 is coupled into the imaging probe assembly 6 after the main unit 9, and i...

Embodiment 2

[0061] Refer Figure 4 Further improvements as in the basis of the first embodiment, in the present embodiment, the imaging probe assembly 6 also includes an input fiber 6.1, a housing 6.7, and a first gradient refractive index lens 6.2 and a diffractive optical element 6.3 disposed within the outer casing 6.7;

[0062] Rotary output unit 6.0.2 includes a bipregular prism having a bipregular prism having a double right angle prism on the outer casing 6.7 and a second gradient refractive index lens 6.5 connected to a double right prism on 6.4a, and the rotary drive unit 6.0.1 includes disposed within the housing 6.7. Micro-rotating electric machine 6.6.2 and rotary shaft 6.6.1 of the output shaft driven by micro-rotating electric machine 6.6.2, bipregular prisms 6.4a are connected to the rotating shaft 6.6.1;

[0063]The femtosecond pulsed laser light emitted by the fiber coupled lens 5 is coupled to the input fiber 6.1, and then the first gradient refractive index lens 6.2 is subje...

Embodiment 3

[0066] Refer Figure 5 Further, as another embodiment of the first embodiment, in the present embodiment, the double-right angle prism and the second gradient refractive index lens 6.5 are replaced with an off-axis parabolic mirror 6.4b, i.e., rotary output unit 6.0.2. An off-axis parabolic mirror 6.4b, the off-axis parabolic mirror 6.4b is connected to the rotating shaft 6.6.1;

[0067] The femtosecond pulsed laser light emitted by the optical fiber coupling lens 5 is coupled to the input fiber 6.1, and then the first gradient refractive index lens 6.2 is sequentially formed, and the diffractive optical element 6.3 performs an axial aberration correction, and is reflected by the off-axis parabolic reflection. The mirror 6.4b is focused and reflected, and a focused spot 6.8 having a high NA is injected, the micro rotary motor 6.6.2 is rotated by the rotation shaft 6.6.1 to rotate high speed, so that the focused spot 6.8 of the emitted is enabled. Scan.

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Abstract

The invention discloses a panoramic rotary endoscopic two-photon microscopic imaging system, which comprises a near-infrared femtosecond pulse laser, a host, an imaging probe assembly and an axial scanning device, wherein the imaging probe assembly comprises a rotary output unit and a rotary driving unit, and the rotary output unit is implanted into the brain tissue of an animal and is used for guiding femtosecond pulse laser into the brain tissue and collecting fluorescence generated when the brain tissue is excited. According to the panoramic rotary endoscopic two-photon microscopic imaging system, high-speed rotary scanning and a miniature endoscopic two-photon imaging system are combined, and the panoramic endoscopic two-photon microscopic imaging system which can be particularly suitable for large primate animals is provided. The rotary output unit of the probe assembly is implanted into the brain tissue, 360-degree panoramic imaging in an observed brain area can be realized by 360-degree annular cylindrical surface scanning, detection at different depths can be performed by axial scanning, and neural activity observation from a deep brain area to a plurality of continuous brain functional areas covered by cerebral cortex pipelines can be realized.

Description

Technical field [0001] The present invention relates to the field of microscopic imaging, and in particular, to a panoramic rotary endo-photon micro-imaging system. Background technique [0002] At present, the introductory neurochuan research is increasingly a key point in brain function in health and disease. In the past decade, new methods and new techniques for supporting the study of meta-neurocenter have achieved rapid development, such as optical calcium imaging and optical mobilization techniques, rapidly become a medium-sized column in the scientific research of neural ring. Optical calcium imaging can record a large amount of genetically or anatomically defined neuronal cells in a high sampling density, and can be pushed over time in longitudinal tracking individual neurons. This powerful optical imaging ability has achieved great success in the animal model of mice, and promotes the brain neuroscientists to understand the basic function of the brain and propose new uni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00G02B21/00
CPCA61B5/0084A61B5/0071A61B5/4064A61B5/6868G02B21/0036G02B21/0076A61B2503/40A61B2503/42
Inventor 唐玉国李敏周镇乔吕晶王艳陈月岩贾宏博
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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