Simultaneous detection and application of volatile pollutant exposure and effect markers in urine

Abstract

The invention discloses a method and application for synchronous detection of volatile pollutant exposure and effect markers in urine. The method selects OH-PAHs, mVOCs and 8-OHdG that may be correlated in the same urine sample as targets Detected substances are extracted, detected and analyzed synchronously, which includes the following steps: sample pretreatment; sample enrichment and separation; OHdG is detected on the machine, and the corresponding analysis is used to obtain the content data of various target detection substances in urine, and the correlation between the three is analyzed. The invention applies the analysis of the relationship between the content data of the target substance to evaluate the human body exposure characteristics of two types of pollutants. The invention enables synchronous detection and analysis of various target detection substances through an optimization scheme, simplifies the detection steps, reduces the detection cost, and expands the application range of the health exposure risk assessment results.

Description

technical field [0001] The invention belongs to the fields of pollutant detection, health exposure risk assessment and medical technology, and specifically relates to the simultaneous detection and application of volatile pollutant exposure and effect markers in urine. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of semi-volatile organic pollutants (SVOCs) produced by incomplete combustion of organic matter such as coal, petroleum, and tobacco. Studies have shown that exposure to PAHs may cause irritation to the digestive tract and respiratory tract, and long-term exposure can also cause damage to the human circulatory system and nervous system, resulting in teratogenic, carcinogenic, and mutagenic hazards. Volatile organic compounds (VOCs) are ubiquitous pollutants in the atmosphere, and their sources are very extensive. Industrial production, motor vehicle exhaust, cigarette smoke, and high-temperature cooking foods all produce VOCs. L...

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned deficiencies of the existing technology, especially for the detection of OH-PAHs, mVOCs and 8-OHdG using the existing detection methods, the sample pretreatment, detection instruments and analysis methods used are all different, and it needs to be divided into three times. To complete the detection problem, provide a new simultaneous detection method of volatile pollutant exposure and effect markers in urine. By improving the sample pretreatment, detection and analysis scheme, these three target detection substances are included in the analysis of one sample. It is completed in one detection process, and it is convenient, fast, and solvent-saving; at the same time, the data of three target detection substances obtained synchronously by one detection of one sample is used. Since the samples and detection conditions have not changed, the obtained data has no error. The correlation between PAHs, VOCs and 8-OHdG can be further analyzed accurately

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