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Method for preparing nano-selenium by compounding polyphenol compound and protein and prepared nano-selenium

A polyphenolic compound and protein technology, applied in the field of nano selenium, can solve the problems of difficulty in giving nano selenium other functions, loss of biological activity, poor stabilization effect, etc.

Active Publication Date: 2021-12-10
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Nano-selenium can generally be prepared by methods such as chemical reduction, solvothermal method, microwave-assisted method, and biosynthesis. Among them, the chemical reduction method is widely used because of its simple operation and low cost, but the nano-selenium synthesized by the chemical reduction method is easy. Agglomeration, loss of biological activity, need to be stabilized by external template
Recently, there are also many literature reports using bioactive substances such as polysaccharides, proteins, and polyphenolic compounds as a soft template to stabilize nano-selenium. Among them, the effect of protein soft templates to stabilize nano-selenium is better, but it is difficult to endow nano-selenium with other functions; polyphenolic compounds Although the soft template can endow nanoselenium with additional functions, the stabilization effect is poor
The method of stabilizing nano-selenium by using polyphenolic compounds and proteins as soft templates has not been reported so far

Method used

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  • Method for preparing nano-selenium by compounding polyphenol compound and protein and prepared nano-selenium
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  • Method for preparing nano-selenium by compounding polyphenol compound and protein and prepared nano-selenium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: A kind of method that utilizes polyphenol compound and protein compound to prepare nano-selenium

[0035] 1) Prepare 100mM sodium selenite solution, 250mM vitamin C solution, 10mg / mL BSA solution, and 10mMEGCG solution respectively.

[0036] 2) The concentration of BSA in the fixed final mixed reaction system is 5mg / mL and the concentration of EGCG is 3mM, first a certain amount of BSA solution and EGCG solution are mixed to obtain the reaction system; the fixed sodium selenite and vitamin C molar ratio is 10:1, according to the concentration of sodium selenite in the final mixed reaction system is 2.5, 5, 7.5, 10, 12.5 and 15mM respectively, first add a certain amount of vitamin C solution to the reaction system, add selenous acid after mixing Sodium solution, stirred for 10 minutes to mix evenly, and left to stand at 25°C for 50 minutes to obtain a nano-selenium colloidal solution;

[0037] 3) Use 8kDa regenerated cellulose dialysis bag to dialyze the a...

Embodiment 2

[0039] Embodiment 2: A kind of method that utilizes polyphenol compound and protein compound to prepare nano-selenium

[0040] Compared with Example 1, the difference is:

[0041] Prepare 500mM vitamin C solution, fix the concentration of sodium selenite in the final mixed reaction system to be 5mM, be 1:4, 1:6, 1:8, 1:10, respectively according to sodium selenite and vitamin C mol ratio 1:12 and 1:16 (mmol:mmol), add a certain amount of 500mM vitamin C solution, mix well, then add 100mM sodium selenite solution, stir for 10min to mix, let stand at 25°C for 50min, and get nano-selenium colloidal solution , Determination of its colloidal chemical properties, the results are as follows figure 2As shown, comprehensively considered, when the molar ratio of sodium selenite to vitamin C in the system is 1:8, the average particle size is smaller and the system is more stable.

Embodiment 3

[0042] Embodiment 3: A kind of method that utilizes polyphenol compound and protein compound to prepare nano-selenium

[0043] Compared with Example 1, the difference is:

[0044] Prepare 30mg / mL BSA solution, according to the concentration of BSA in the final mixed reaction system is 1, 3, 5, 7 and 9mg / mL, mix a certain amount of 30mg / mL BSA solution with 10mM EGCG solution, and fix the final mixed The concentration of sodium selenite in the reaction system is 5mM, according to the molar ratio of sodium selenite and vitamin C is 1:8 (mmol:mmol), first add a certain amount of 250mM vitamin C solution, after mixing, add a certain amount of 100mM selenous NaCl solution, stirred for 10min to mix, left to stand at 25°C for 50min to obtain nano-selenium colloidal solution, and measured its colloidal chemical properties, the results were as follows image 3 As shown, considering comprehensively, when the concentration of BSA in the mixed reaction system is 3mg / mL, the average parti...

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Abstract

The invention belongs to the technical field of nano-materials, and discloses a method for preparing nano-selenium by compounding a polyphenol compound and protein and the prepared nano-selenium. The method comprises the following steps: (1) compounding a polyphenol compound and protein into a soft template: uniformly mixing a protein solution with a certain concentration with a polyphenol compound solution, and directly using or freeze-drying for use; and (2) preparing nano-selenium by using the polyphenol-protein soft template: adding a vitamin C solution into a reaction system in which the polyphenol-protein soft template exists, uniformly mixing, then adding a sodium selenite solution, uniformly stirring and mixing, standing the obtained mixed reaction system for reduction reaction to obtain nano-selenium sol, dialyzing or centrifugally separating to obtain nano-selenium suspension, and freeze-drying to obtain the nano-selenium solid. According to the invention, the polyphenol compound and the protein are compounded into the soft template, so that the functional characteristics of the nano-selenium are enhanced, the stability of the nano-selenium is also enhanced, the application field of the nano-selenium is widened, and the application value of the nano-selenium is improved.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a method for preparing nano-selenium by compounding polyphenolic compounds and proteins and the prepared nano-selenium. Background technique [0002] Selenium (Se) is one of the essential trace elements for the human body and a key component of various enzyme activity centers in the human body. It plays a role in resisting diseases, delaying aging, and enhancing immune function in life. Selenium cannot be synthesized by the human body and can only be supplemented through diet. However, the safe dose range of selenium element is relatively narrow, and it is easy to cause toxicity due to excessive intake. Traditional selenium supplements are mostly inorganic selenium (sodium selenite) and organic selenium (selenomethionine, etc.). Studies have shown that the toxicity of selenium and its compounds is: inorganic selenium > organic selenium > elemental selenium...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B19/02
CPCC01B19/02
Inventor 林晓蓉郑晓峰李斌叶锡光黄欣琪陈忠正张媛媛
Owner SOUTH CHINA AGRI UNIV
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