Polygonum viviparum extract as well as preparation method and application thereof

A technology of Polygonum vulgaris and its extract is applied to the rhizomes of Polygonum vulgaris, fruit extracts and their preparation, and the fields of stems and leaves to achieve the effect of removing tyrosinase activity

Active Publication Date: 2021-12-10
DONGGUAN HEC CORDYCEPS R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are very few literatures on the research on the use of Polygonum viburnum, and most of them are clinical cases of treating diarrhea. There are also several research reports on the antioxidant effect.

Method used

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  • Polygonum viviparum extract as well as preparation method and application thereof
  • Polygonum viviparum extract as well as preparation method and application thereof
  • Polygonum viviparum extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1: Preparation of Polygonum viburnum extract

[0078] 1. Experimental reagents: anhydrous methanol (Chengdu Kelong, AR, 2020072302); ODS (Japan YMC Company, AQG12S50).

[0079] 2. Source of medicinal materials: The rhizome of Polygonum villosa comes from Yunnan, the fruit comes from Sichuan, and the stems and leaves come from Tibet.

[0080] 3. Experimental instruments: ultrapure water is self-made in the laboratory (Milli-Q ultrapure water instrument of Millipore Corporation of the United States); 1 / 100,000 electronic balance XS205 (Switzerland Mettler-Toledo Instrument Co., Ltd.).

[0081] 4. Preparation method:

[0082] 1) Grind the dried rhizomes, stems and leaves, and fruits of Polygonum versicolor respectively, weigh 0.5 g of each sample powder, add 10 mL of 50% methanol (V / V) for ultrasonic extraction for 20 minutes, and obtain the rhizomes of Polygonum vermilion after rotary evaporation to dryness Total extract (TRE), total stem and leaf extract (TL...

Embodiment 2

[0084] Example 2: Determination of α-glucosidase inhibitory activity

[0085] 1. Experimental instrument: multifunctional microplate reader (Biotek).

[0086] 2. Experimental reagents: α-glucosidase (Sigma, 750UN, SLCC4854); Potassium dihydrogen phosphate (Guangdong Guanghua, HPLC, 20190427); Sodium hydroxide (Xilong Science, AR, 191115); PNPG (Yuan Le Biology, 99.8%, K17A10B82914); sodium carbonate (Xilong Science, AR, 180408); acarbose (Aladdin, 100%, A1823131).

[0087] 3. Experimental solution: phosphate buffer (pH=6.8); α-glucosidase solution (0.15U / mL); PNPG (p-nitrophenyl-α-D-glucopyranoside) solution (0.5mg / mL); sodium carbonate solution (1mol / L); acarbose positive control (2.00, 1.00, 0.50, 0.25 mg / mL).

[0088] 4. Experimental samples: TRE, TLE, TFE, R-15% MeOH, R-60% MeOH, R-100% MeOH, L-15% MeOH, L-60% MeOH, L-100% MeOH, F-15 %MeOH, F-60% MeOH and F-100% MeOH.

[0089] 5. Experimental method:

[0090] 1) Preparation of the test solution

[0091] Take an appr...

Embodiment 3

[0118] Example 3: Determination of tyrosinase inhibitory activity

[0119] 1. Experimental equipment: multi-functional microplate reader (Biotek); constant temperature water bath (Shanghai Yiheng).

[0120] 2. Experimental reagents: potassium dihydrogen phosphate (Guangdong Guanghua, AR, 1807061); sodium hydroxide (Xilong science, AR, 20180103); tyrosine (Sigma, SLBV3552); tyrosinase (Sigma, 25KU, SLBZ0022 ), arbutin (Guangzhou Jueran, 201712002A).

[0121] 3. Experimental solution: prepare tyrosine solution (5.0μmol / mL); tyrosinase solution (200U / mL); phosphate buffer (pH=6.50); ​​arbutin positive control solution (4.03600, 2.01800, 1.00900 , 0.50450mg / mL).

[0122] 4. Experimental samples: TRE, TLE, TFE, R-15% MeOH, R-60% MeOH, R-100% MeOH, L-15% MeOH, L-60% MeOH, L-100% MeOH, F-15 %MeOH, F-60% MeOH and F-100% MeOH.

[0123] 5. Experimental method:

[0124] 1) Preparation of the test solution

[0125] Take an appropriate amount of dry extracts, dissolve them with 3% meth...

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PUM

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Abstract

The invention relates to the technical field of medicinal material extraction, in particular to polygonum viviparum rhizome, stem leaf and fruit extracts as well as a preparation method and application thereof. The invention provides a preparation method of the polygonum viviparum rhizome, stem leaf and fruit extracts. The polygonum viviparum rhizome, stem leaf and fruit extracts prepared by the method have the effects of inhibiting the activity of alpha-glucosidase and tyrosinase and removing ABTS free radicals.

Description

technical field [0001] The present invention relates to the technical field of medicinal material extraction, in particular, the present invention relates to Polygonum villosa rhizome, stem and leaf, fruit extract and its preparation method and application. Background technique [0002] Enzyme inhibitors refer to substances that can act on certain groups of enzymes, reduce the activity of enzymes or completely inactivate enzymes, so as to obtain a certain therapeutic effect. α-glucosidase inhibitors can inhibit the activity of α-glucosidase in small intestinal epithelial cells, slow down the hydrolysis of oligosaccharides in food into glucose, reduce the absorption of glucose by small intestinal epithelial cells, and achieve the purpose of lowering blood sugar and treating diabetes . Tyrosinase inhibitors can inhibit the activity of tyrosinase in melanocytes, reduce the synthesis of dopachrome, reduce the deposition of melanin in the skin, so as to achieve the effect of whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/704A61P39/06A61P3/10A61P17/00A61K125/00A61K135/00A61K127/00A61K131/00
CPCA61K36/704A61P39/06A61P3/10A61P17/00A61K2236/15A61K2236/55A61K2236/51Y02A50/30
Inventor 何焯斌钱正明黄琦王巧贺媛
Owner DONGGUAN HEC CORDYCEPS R&D CO LTD
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