Construction method and application of animal model for detecting digestion and absorption characteristic of protein gel

Abstract

The invention relates to a construction method and application of an animal model for detecting digestion and absorption characteristics of protein gel. The method comprises the steps: selecting male SD rats, placing each rat independently in a metabolism cage, adaptively feeding the rats with standard feed for one week, and performing fasting for 24 hours; starting to feed a protein gel sample for a period of time, feeding the rats at fixed time every day, freely drinking water, collecting residual samples at the same time in the morning next day, weighing, discarding, and feeding for another time; after 24 hours of fasting, feeding each rat with the same sample feeding time, taking out the rats after 3-4 hours, killing the rats with isoflurane, immediately collecting blood from aorta abdominalis, and collecting stomach digests and intestine digests from the stomach and the small intestine; and completing construction of the animal model for detecting the digestion characteristics of the protein gel. Compared with an intragastric administration method and a mixed feed method, the method is more suitable for researching the digestion and absorption characteristics of a protein gel food, and the influence of the structure of the protein gel food on the digestion and absorption characteristics can be researched through the method.

Description

technical field [0001] The invention relates to the technical field of medium detection, and relates to a construction method and application of an animal model for detecting protein gel digestion and absorption characteristics. Background technique [0002] With the transformation of people's consumption concept and the continuous improvement of the pursuit of healthy food, it is necessary to evaluate the digestion and absorption characteristics of food. Common digestion methods are divided into in vitro simulated digestion and animal in vivo digestion. [0003] Wen S, Zhou G, Song S, et al.Discrimination of in vitro and in vivodigestion products of meat proteins from pork,beef,chicken,and fish[J].Proteomics,2015,15(21):3688-3698 published The method is: the gel behavior of double protein milk under the co-induction condition of lactic acid bacteria and MTG enzyme, and the research on the digestibility and antigenicity of the protein in vitro. [0004] Gallier S, Cui J, O...

AI Technical Summary

Problems solved by technology

At present, the commonly used feeding methods are gavage method or mixed feeding of experimental samples and standard feed, but gavage method is only suitable for liquid samples or easily soluble samples, not for gel food, and the protein is extracted to make mixed feed Therefore, it is necessary to design an animal model suitable for the digestion of protein gel foods in rats.

[0006] At the same time, there is currently a lack of a set of programs for evaluating the digestive properties of protein gel foods, including feed conversion rate, protein digestibility, digestion kinetics, peptidomic analysis of digested products, amino acid composition analysis, and serum metabolomic analysis Wait

Many studies only focus on a few of them, but cannot complete and systematically analyze their digestive characteristics. Therefore, the present invention proposes a digestion and absorption scheme suitable for protein gel foods in rats. Nutritional properties of gel foods provide reference

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